Construct: ORF TRCN0000473232

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF007671.1_s317c1
Derived from:
ccsbBroadEn_15762
DNA Barcode:
CCTCTGTTATACTCCGCTGCAGGG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
QPRT (23475)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000473232
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 23475 QPRT quinolinate phosphoribosylt... NM_014298.5 100% 100%
2 human 23475 QPRT quinolinate phosphoribosylt... XM_017023101.1 78.2% 78.1% 1_246del;829A>G
3 human 23475 QPRT quinolinate phosphoribosylt... XM_005255223.3 78% 77.8% 1_246del;794_796delAGC;832A>G
4 human 23475 QPRT quinolinate phosphoribosylt... NM_001318250.1 50.1% 50.1% 104_105ins444
5 human 23475 QPRT quinolinate phosphoribosylt... NM_001318249.1 47.8% 39.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
957
ORF length:
891
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgga cgctgaaggc ctggcgctgc tgctgccgcc cgtcaccctg gcagccctgg
121tggacagctg gctccgagag gactgcccag ggctcaacta cgcagccttg gtcagcgggg
181caggcccctc gcaggcggcg ctgtgggcca aatcccctgg ggtactggca gggcagcctt
241tcttcgatgc catatttacc caactcaact gccaagtctc ctggttcctc cccgagggat
301cgaagctggt gccggtggcc agagtggccg aggtccgggg ccctgcccac tgcctgctgc
361tgggggaacg ggtggccctc aacacgctgg cccgctgcag tggcattgcc agtgctgccg
421ccgctgcagt ggaggccgcc aggggggccg gctggactgg gcacgtggca ggcacgagga
481agaccacgcc aggcttccgg ctggtggaga agtatgggct cctggtgggc ggggccgcct
541cgcaccgcTA CGACCTGGGA GGGCTGGTGA TGGTGAAGGA TAACCATGTG GTGGCCGCCG
601GTGGCGTGGA GAAGGCGGTG CGGGCGGCCA GACAGGCGGC TGACTTCGCT CTGAAGGTGG
661AAGTGGAATG CAGCAGCCTG CAGGAGGCCG TGCAGGCAGC TGAGGCTGGT GCCGACCTTG
721TCCTGCTGGA CAACTTCAAG CCAGAGGAGC TGCACCCCAC GGCCACCGTG CTGAAGGCCC
781AGTTCCCGAG TGTGGCTGTG GAAGCCAGTG GGGGCATCAC CCTGGACAAC CTCCCCCAGT
841TCTGCGGGCC GCACATAGAC GTCATCTCCA TGGGGATGCT GACCCAGGCG GCCCCAGCCC
901TTGATTTCTC CCTCAAGCTG TTTGCCAAAG AGGTGGCTCC AGTGCCCAAA ATCCACTACC
961CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC
1021TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT
1081ATATATCTTG TGGAAAGGAC GACCTCTGTT ATACTCCGCT GCAGGGACGC GTTAAGTCga
1141caatcaacct ctggattaca aaatttgtga aagatt

Download FASTA (ORF) (Full)