Transcript: Human NM_001318249.1

Homo sapiens quinolinate phosphoribosyltransferase (QPRT), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-09-25
Taxon:
Homo sapiens (human)
Gene:
QPRT (23475)
Length:
1849
CDS:
90..548

Additional Resources:

NCBI RefSeq record:
NM_001318249.1
NBCI Gene record:
QPRT (23475)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001318249.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000376580 CTTCGCTCTGAAGGTGGAAGT pLKO_005 233 CDS 100% 4.050 5.670 N QPRT n/a
2 TRCN0000370218 GCTCATCTCAGTTTCCTAATC pLKO_005 723 3UTR 100% 10.800 7.560 N QPRT n/a
3 TRCN0000034845 AGCCCTTGATTTCTCCCTCAA pLKO.1 485 CDS 100% 4.050 2.835 N QPRT n/a
4 TRCN0000034847 TCCCTCAAGCTGTTTGCCAAA pLKO.1 498 CDS 100% 4.050 2.835 N QPRT n/a
5 TRCN0000377477 CTAGTCCTAAACCGGAAGAGG pLKO_005 545 CDS 100% 0.000 0.000 N QPRT n/a
6 TRCN0000007228 CACCTGTAATCCCAGCACTTT pLKO.1 860 3UTR 100% 4.950 2.475 Y CFLAR n/a
7 TRCN0000166635 CACCTGTAATCCCAGCACTTT pLKO.1 860 3UTR 100% 4.950 2.475 Y C19orf31 n/a
8 TRCN0000021429 CACACCTGTAATCCCAGCATT pLKO.1 858 3UTR 100% 4.950 2.475 Y ERN2 n/a
9 TRCN0000138998 CACACCTGTAATCCCAGCATT pLKO.1 858 3UTR 100% 4.950 2.475 Y P3H4 n/a
10 TRCN0000344020 CACACCTGTAATCCCAGCATT pLKO_005 858 3UTR 100% 4.950 2.475 Y P3H4 n/a
11 TRCN0000138772 GCAGGAGAATCGCTTGAACTT pLKO.1 1027 3UTR 100% 4.950 2.475 Y DCAF11 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001318249.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15762 pDONR223 0% 47.8% 39.6% None (many diffs) n/a
2 ccsbBroad304_15762 pLX_304 0% 47.8% 39.6% V5 (many diffs) n/a
3 TRCN0000473232 CCTCTGTTATACTCCGCTGCAGGG pLX_317 45.9% 47.8% 39.6% V5 (many diffs) n/a
4 ccsbBroadEn_07889 pDONR223 100% 47.8% 39.6% None (many diffs) n/a
5 ccsbBroad304_07889 pLX_304 0% 47.8% 39.6% V5 (many diffs) n/a
6 TRCN0000466332 TAGGTTGCAGCCCCCAGTGCCACG pLX_317 31.7% 47.8% 39.6% V5 (many diffs) n/a
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