Construct: ORF TRCN0000473237

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF001394.1_s317c1
Derived from:
ccsbBroadEn_15377
DNA Barcode:
CGTACTAATCGTGAAATTTGATAT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CBS (875)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000473237
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 875 CBS cystathionine beta-synthase NM_000071.2 99.9% 99.8% 205G>C
2 human 875 CBS cystathionine beta-synthase NM_001178008.2 99.9% 99.8% 205G>C
3 human 875 CBS cystathionine beta-synthase NM_001178009.3 99.9% 99.8% 205G>C
4 human 875 CBS cystathionine beta-synthase NM_001320298.1 99.9% 99.8% 205G>C
5 human 875 CBS cystathionine beta-synthase XM_017028491.2 99.9% 99.8% 205G>C
6 human 102724560 CBSL cystathionine beta-synthase... NM_001321073.2 99.8% 99.8% 205G>C;699T>C
7 human 102724560 CBSL cystathionine beta-synthase... NM_001354006.1 99.8% 99.8% 205G>C;699T>C
8 human 102724560 CBSL cystathionine beta-synthase... NM_001354007.1 99.8% 99.8% 205G>C;699T>C
9 human 102724560 CBSL cystathionine beta-synthase... NM_001354008.1 99.8% 99.8% 205G>C;699T>C
10 human 102724560 CBSL cystathionine beta-synthase... NM_001354009.1 99.8% 99.8% 205G>C;699T>C
11 human 102724560 CBSL cystathionine beta-synthase... NM_001354010.1 99.8% 99.8% 205G>C;699T>C
12 human 102724560 CBSL cystathionine beta-synthase... NM_001354012.1 99.8% 99.8% 205G>C;699T>C
13 human 875 CBS cystathionine beta-synthase XM_011529777.2 97.4% 97.1% 205G>C;1553_1594del
14 human 102724560 CBSL cystathionine beta-synthase... NM_001354014.1 97.4% 97.1% 205G>C;699T>C;1553_1594del
15 human 102724560 CBSL cystathionine beta-synthase... NM_001354015.1 97.4% 97.1% 205G>C;699T>C;1553_1594del
16 human 875 CBS cystathionine beta-synthase XM_024452137.1 85.3% 77.8% (many diffs)
17 human 102724560 CBSL cystathionine beta-synthase... XM_017028209.1 85.1% 77.8% (many diffs)
18 human 102724560 CBSL cystathionine beta-synthase... XM_017028210.1 85.1% 77.8% (many diffs)
19 human 875 CBS cystathionine beta-synthase XM_011529774.2 83.3% 75.9% (many diffs)
20 human 875 CBS cystathionine beta-synthase XM_024452136.1 83.3% 75.9% (many diffs)
21 human 875 CBS cystathionine beta-synthase NM_001321072.1 80.8% 80.7% 1_1delAins316
22 human 875 CBS cystathionine beta-synthase XM_024452138.1 80.8% 80.7% 1_1delAins316
23 human 875 CBS cystathionine beta-synthase XM_024452139.1 80.8% 80.7% 1_1delAins316
24 human 875 CBS cystathionine beta-synthase XM_024452140.1 80.8% 80.7% 1_1delAins316
25 human 875 CBS cystathionine beta-synthase XR_001754917.2 80.8% (many diffs)
26 human 875 CBS cystathionine beta-synthase XM_011529783.2 78.8% 78.5% 1_1delAins316;1238_1279del
27 human 875 CBS cystathionine beta-synthase XR_001754916.2 67.8% (many diffs)
28 human 102724560 CBSL cystathionine beta-synthase... NR_148682.1 60.6% (many diffs)
29 human 875 CBS cystathionine beta-synthase XR_001754915.1 53.1% (many diffs)
30 human 875 CBS cystathionine beta-synthase XR_002958634.1 47.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1719
ORF length:
1653
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgcc ttctgagacc ccccaggcag aagtggggcc cacaggctgc ccccaccgct
121cagggccaca ctcggcgaag gggagcctgg agaaggggtc cccagaggat aaggaagcca
181aggagcccct gtggatccgg cccgatgctc cgagcaggtg cacctggcag ctgggccggc
241ctgcctccga gtccccacat caccacactc ccccggcaaa atctccaaaa atcttgccag
301atattctgaa gaaaatcggg gacaccccta tggtcagaat caacaagatt gggaagaagt
361tcggcctgaa gtgtgagctc ttggccaagt gtgagttctt caacgcgggc gggagcgtga
421aggaccgcat cagcctgcgg atgattgagg atgctgagcg cgacgggacg ctgaagcccg
481gggacacgat tatcgagccg acatccggga acaccgggat cgggctggcc ctggctgcgg
541cagtgagggg ctatcgctgc atcatcgtga tgccagagaa gatgagctcc gagaaggtgg
601acgtgctgcg ggcactgggg gctgagattg tgaggacgcc caccaatgcc aggttcgact
661ccccggagtc acacgtgggg gtggcctggc ggctgaagaa cgaaatcccc aattctcaca
721tcctagacca gtaccgcaac gccagcaacc ccctggctca ctacgacacc accgctgatg
781agatcctgca gcagtgtgat gggaagctgg acatgctggt ggcttcagtg ggcacgggcg
841gcaccatcac gggcattgcc aggaagctga aggagaagtg tcctggatgc aggatcattg
901gggtggatcc cgaagggtcc atcctcgcag agccggagga gctgaaccag acggagcaga
961caacctacga ggtggaaggg atcggctacg acttcatccc cacggtgctg gacaggacgg
1021tggtggacaa gtggttcaag agcaacgatg aggaggcgtt cacctttgcc cgcatgctga
1081tcgcgcaaga ggggctgctg tgcggtggca gtgctggcag cacggtggcg gtggccgtga
1141aggccgcgca ggagctgcag gagggccagc gctgcgtggt cattctgccc gactcagtgc
1201ggaactacat gaccaagttc ctgagcgaca ggtggatgct gcagaagggc tttctgaagg
1261aggaggacct cacggagaag aagccctggt ggtggcacct ccgtgttcag gagctgggcc
1321tgtcagcccc gctgaccgtg ctcccgacca tcacctgtgg gcacaccatc gagatccTCC
1381GGGAGAAGGG CTTCGACCAG GCGCCCGTGG TGGATGAGGC GGGGGTAATC CTGGGAATGG
1441TGACGCTTGG GAACATGCTC TCGTCCCTGC TTGCCGGGAA GGTGCAGCCG TCAGACCAAG
1501TTGGCAAAGT CATCTACAAG CAGTTCAAAC AGATCCGCCT CACGGACACG CTGGGCAGGC
1561TCTCGCACAT CCTGGAGATG GACCACTTCG CCCTGGTGGT GCACGAGCAG ATCCAGTACC
1621ACAGCACCGG GAAGTCCAGT CAGCGGCAGA TGGTGTTCGG GGTGGTCACC GCCATTGACT
1681TGCTGAACTT CGTGGCCGCC CAGGAGCGGG ACCAGAAGTG CCCAACTTTC TTGTACAAAG
1741TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT
1801GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG
1861ACGACGTACT AATCGTGAAA TTTGATATAC GCGTTAAGTC gacaatcaac ctctggatta
1921caaaatttgt gaaagatt

Download FASTA (ORF) (Full)