Construct: ORF TRCN0000473250
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013330.1_s317c1
- Derived from:
- ccsbBroadEn_11365
- DNA Barcode:
- TCCTATCACACGCACTATCTAATG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SELENOF (9403)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473250
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9403 | SELENOF | selenoprotein F | NM_004261.5 | 38.1% | 0% | 1_256del;313_362del |
| 2 | human | 9403 | SELENOF | selenoprotein F | NM_203341.2 | 26% | 0% | 1_256del;372_373ins73 |
| 3 | human | 9403 | SELENOF | selenoprotein F | NR_144513.1 | 11.7% | 1_317del;374_423del;557_1603del | |
| 4 | human | 9403 | SELENOF | selenoprotein F | NR_144512.1 | 11.6% | 1_333del;390_439del;573_1619del | |
| 5 | mouse | 93684 | Selenof | selenoprotein F | NM_053102.2 | 35.3% | 7.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 314
- ORF end:
- 503
- ORF length:
- 189
- Sequence:
-
1 GTCGCTTCAT GTGACTCCAC GGAGTACCGG GCGCCGTCCA GGCACCTCGA TTAGTTCTCG 61 AGCTTTTGGA GTACGTCGTC TTTAGGTTGG GGGGAGGGGT TTTATGCGAT GGAGTTTCCC 121 CACACTGAGT GGGTGGAGAC TGAAGTTAGG CCAGCTTGGC ACTTGATGTA ATTCTCCTTG 181 GAATTTGCCC TTTTTGAGTT TGGATCTTGG TTCATTCTCA AGCCTCAGAC AGTGGTTCAA 241 AGTTTTTTTC TTCCATTTCA GGTGTCGTGA GGCTAGCATC GATTGATCAA CAAGTTTGTA 301 CAAAAAAGTT GGCATGCAGG AGCTATTCTT GAAGTTTGTG GATGAAAATT GGGAAGGTTC 361 CCTCAAGTCC AAGTATGTCC GTGGTTCAGA CCCTGTATTA AAGCTTTTGG ACGACAATGG 421 GAACATTGCT GAAGAACTGA GCATTCTCAA ATGGAACACA GACAGTGTAG AAGAATTCCT 481 GAGTGAAAAG TTGGAACGCA TATACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA 541 GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT 601 TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATC CTATCACACG 661 CACTATCTAA TGACGCGTTA AGTCgacaat caacctctgg attacaaaat ttgtgaaaga 721 tt