Construct: ORF TRCN0000473251
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008358.1_s317c1
- Derived from:
- ccsbBroadEn_04835
- DNA Barcode:
- ATTGTCGATTTCGCCATGACTACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SPATA17 (128153)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473251
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 128153 | SPATA17 | spermatogenesis associated 17 | NM_138796.4 | 99.9% | 100% | 582G>A |
| 2 | human | 128153 | SPATA17 | spermatogenesis associated 17 | XM_005273052.1 | 99.9% | 100% | 582G>A |
| 3 | human | 128153 | SPATA17 | spermatogenesis associated 17 | XM_006711165.3 | 95.1% | 95.2% | 239_240ins51;531G>A |
| 4 | human | 128153 | SPATA17 | spermatogenesis associated 17 | XM_011509194.3 | 56.3% | 47.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1149
- ORF length:
- 1083
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cacgttagcc cggctgcaag ctaggtcgtc gactgtagga aatcagtact 121 actttaggaa cagtgttgta gatccattta gaaaaaagga gaatgatgca gcagttaaaa 181 tccaaagctg gtttcgagga tgtcaagttc gggcatatat caggcattta aacaggattg 241 taacaattat tcaaaaatgg tggagaagtt tcttaggcag aaagcaatat caactaactg 301 tgcaggtagc atattatact atgatgatga atctctacaa tgcaatggct gtcaggattc 361 agagacgatg gcgaggctat agggttcgga agtacctctt taattattat tatttgaaag 421 agtacctgaa agtcgtttca gagaccaatg atgcaattag gaaggcactg gaggagtttg 481 cagaaatgaa agaaagagaa gagaagaagg ctaacctcga aagggaagag aagaaaagag 541 attaccaagc ccgaaagatg cattacctcc tcagcacaaa gcagattcca ggaatataca 601 attcaccctt cagaaaagag cctgatCCAT GGGAGCTGCA ATTACAAAAG GCAAAGCCTT 661 TAACACACCG AAGACCTAAA GTTAAGCAGA AGGACTCCAC CAGCCTTACT GATTGGCTAG 721 CTTGTACAAG CGCCCGTTCT TTTCCTCGGT CTGAAATTCT ACCACCTATT AATAGAAAGC 781 AATGTCAGGG GCCCTTCCGA GATATCACCG AAGTATTAGA ACAACGCTAC AGGCCTTTGG 841 AGCCAACGTT GCGGGTGGCA GAACCAATCG ATGAGTTAAA GTTGGCCAGA GAGGAGCTCA 901 GAAGAGAGGA ATGGCTGCAA AATGTAAATG ACAATATGTT TTTGCCATTT TCTTCATACC 961 ATAAAAATGA AAAGTACATC CCATCAATGC ATTTATCAAG CAAGTATGGT CCTATTTCTT 1021 ACAAAGAACA ATTCCGAAGT GAAAATCCTA AGAAATGGAT CTGTGACAAG GATTTCCAGA 1081 CTGTATTACC ATCATTTGAG CTCTTCTCAA AGTATGGAAA ATTATATTCA AAAGCTGGAC 1141 AGATTGTATA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1201 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1261 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAATTGTC GATTTCGCCA TGACTACCAC 1321 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt