Construct: ORF TRCN0000473257
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001582.1_s317c1
- Derived from:
- ccsbBroadEn_06335
- DNA Barcode:
- CCAGAGGACCTAGAGCAGGACAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GSTP1 (2950)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473257
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2950 | GSTP1 | glutathione S-transferase pi 1 | NM_000852.4 | 99.6% | 99.5% | 313A>G;555T>C |
| 2 | mouse | 14870 | Gstp1 | glutathione S-transferase, ... | NM_013541.1 | 83.4% | 85.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 696
- ORF length:
- 630
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc gccctacacc gtggtctatt tcccagttcg aggccgctgc gcggccctgc 121 gcatgctgct ggcagatcag ggccagagct ggaaggagga ggtggtgacc gtggagacgt 181 ggcaggaggg ctcactcaaa gcctcctgcc tatacgggca gctccccaag ttccaggacg 241 gagacctcac cctgtaccag tccaatacca tccTGCGTCA CCTGGGCCGC ACCCTTGGGC 301 TCTATGGGAA GGACCAGCAG GAGGCAGCCC TGGTGGACAT GGTGAATGAC GGCGTGGAGG 361 ACCTCCGCTG CAAATACGTC TCCCTCATCT ACACCAACTA TGAGGCGGGC AAGGATGACT 421 ATGTGAAGGC ACTGCCCGGG CAACTGAAGC CTTTTGAGAC CCTGCTGTCC CAGAACCAGG 481 GAGGCAAGAC CTTCATTGTG GGAGACCAGA TCTCCTTCGC TGACTACAAC CTGCTGGACT 541 TGCTGCTGAT CCATGAGGTC CTAGCCCCTG GCTGCCTGGA TGCGTTCCCC CTGCTCTCAG 601 CATATGTGGG GCGCCTCAGC GCCCGGCCCA AGCTCAAGGC CTTCCTGGCC TCCCCTGAGT 661 ACGTGAACCT CCCCATCAAT GGCAACGGGA AACAGTGCCC AACTTTCTTG TACAAAGTGG 721 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 781 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 841 ACCAGAGGAC CTAGAGCAGG ACAACACGCG TTAAGTCgac aatcaacctc tggattacaa 901 aatttgtgaa agatt