Construct: ORF TRCN0000473260
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008441.1_s317c1
- Derived from:
- ccsbBroadEn_11948
- DNA Barcode:
- TCGGATCCGGCAGAAAGACGTTCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TRNT1 (51095)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473260
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51095 | TRNT1 | tRNA nucleotidyl transferase 1 | NR_159942.2 | 17.4% | 1_78del;146C>T;250_975del | |
| 2 | human | 51095 | TRNT1 | tRNA nucleotidyl transferase 1 | NR_159944.2 | 13.3% | 1_377del;445C>T;549_1274del | |
| 3 | human | 51095 | TRNT1 | tRNA nucleotidyl transferase 1 | NM_001302946.2 | 12.8% | 12% | (many diffs) |
| 4 | human | 51095 | TRNT1 | tRNA nucleotidyl transferase 1 | NM_001367321.1 | 12.2% | 11.4% | (many diffs) |
| 5 | human | 51095 | TRNT1 | tRNA nucleotidyl transferase 1 | NM_001367322.1 | 12.2% | 11.4% | (many diffs) |
| 6 | human | 51095 | TRNT1 | tRNA nucleotidyl transferase 1 | NM_001367323.1 | 12.2% | 11.4% | (many diffs) |
| 7 | human | 51095 | TRNT1 | tRNA nucleotidyl transferase 1 | NM_182916.3 | 12.2% | 11.4% | (many diffs) |
| 8 | human | 51095 | TRNT1 | tRNA nucleotidyl transferase 1 | NR_159943.2 | 8.4% | 1_1106del;1174C>T;1278_2003del | |
| 9 | human | 51095 | TRNT1 | tRNA nucleotidyl transferase 1 | NR_159938.1 | 7.7% | (many diffs) | |
| 10 | human | 51095 | TRNT1 | tRNA nucleotidyl transferase 1 | NR_159939.1 | 7.5% | (many diffs) | |
| 11 | human | 51095 | TRNT1 | tRNA nucleotidyl transferase 1 | NR_159940.1 | 7.5% | (many diffs) | |
| 12 | human | 51095 | TRNT1 | tRNA nucleotidyl transferase 1 | XR_001740168.2 | 6.2% | (many diffs) | |
| 13 | human | 51095 | TRNT1 | tRNA nucleotidyl transferase 1 | NR_159935.1 | 6.1% | (many diffs) | |
| 14 | human | 51095 | TRNT1 | tRNA nucleotidyl transferase 1 | NR_159941.1 | 4.8% | (many diffs) | |
| 15 | human | 51095 | TRNT1 | tRNA nucleotidyl transferase 1 | XR_001740169.2 | 4.5% | (many diffs) | |
| 16 | human | 51095 | TRNT1 | tRNA nucleotidyl transferase 1 | NR_159936.1 | 3.7% | (many diffs) | |
| 17 | human | 51095 | TRNT1 | tRNA nucleotidyl transferase 1 | NR_159934.1 | 3.5% | (many diffs) | |
| 18 | human | 51095 | TRNT1 | tRNA nucleotidyl transferase 1 | NR_159937.1 | 2.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 222
- ORF end:
- 393
- ORF length:
- 171
- Sequence:
-
1 GGAGGGGTTT TATGCGATGG AGTTTCCCCA CACTGAGTGG GTGGAGACTG AAGTTAGGCC 61 AGCTTGGCAC TTGATGTAAT TCTCCTTGGA ATTTGCCCTT TTTGAGTTTG GATCTTGGTT 121 CATTCTCAAG CCTCAGACAG TGGTTCAAAG TTTTTTTCTT CCATTTCAGG TGTCGTGAGG 181 CTAGCATCGA TTGATCAACA AGTTTGTACA AAAAAGTTGG CATGCTGAGG TGCCTGTATC 241 ATTGGCACAG GCCAGTGCTG AACCGTAGGT GGAGTAGGCT GTGCCTTCTG AAGCAGTATC 301 TATTCACAAT GAAGTTGCAG TCTCCCGAAT TCCAGTCACT TTTCACAGAA GGACTGAAGA 361 GTCTGACAGG TATAAATGCT TTCCATGAGA ACTGCCCAAC TTTCTTGTAC AAAGTGGTTG 421 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 481 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATC 541 GGATCCGGCA GAAAGACGTT CTACGCGTTA AGTCgacaat caacctctgg attacaaaat 601 ttgtgaaaga tt