Construct: ORF TRCN0000473266
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000359.1_s317c1
- Derived from:
- ccsbBroadEn_07835
- DNA Barcode:
- GCCTACTCAAGCGCAAGCGAATGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZNF423 (23090)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473266
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23090 | ZNF423 | zinc finger protein 423 | NM_015069.4 | 99.9% | 100% | 543T>C;1902C>T;2673T>C |
| 2 | human | 23090 | ZNF423 | zinc finger protein 423 | XM_017023076.2 | 99.1% | 98.9% | (many diffs) |
| 3 | human | 23090 | ZNF423 | zinc finger protein 423 | XM_006721171.4 | 98.7% | 98.4% | (many diffs) |
| 4 | human | 23090 | ZNF423 | zinc finger protein 423 | NM_001271620.2 | 95.2% | 95.3% | (many diffs) |
| 5 | human | 23090 | ZNF423 | zinc finger protein 423 | XM_005255856.4 | 95.2% | 95.3% | (many diffs) |
| 6 | human | 23090 | ZNF423 | zinc finger protein 423 | XM_017023077.1 | 95.2% | 95.3% | (many diffs) |
| 7 | human | 23090 | ZNF423 | zinc finger protein 423 | XM_017023078.1 | 95.2% | 95.3% | (many diffs) |
| 8 | human | 23090 | ZNF423 | zinc finger protein 423 | NM_001330533.2 | 90.8% | 90.8% | (many diffs) |
| 9 | mouse | 94187 | Zfp423 | zinc finger protein 423 | NM_033327.2 | 90.9% | 97.2% | (many diffs) |
| 10 | mouse | 94187 | Zfp423 | zinc finger protein 423 | XM_017313033.1 | 90.8% | 97% | (many diffs) |
| 11 | mouse | 94187 | Zfp423 | zinc finger protein 423 | XM_011248560.2 | 89.6% | 95.8% | (many diffs) |
| 12 | mouse | 94187 | Zfp423 | zinc finger protein 423 | NM_001310520.1 | 82.9% | 89.4% | (many diffs) |
| 13 | mouse | 94187 | Zfp423 | zinc finger protein 423 | XM_006531539.3 | 82.9% | 89.4% | (many diffs) |
| 14 | mouse | 94187 | Zfp423 | zinc finger protein 423 | XM_011248561.2 | 82.9% | 89.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3921
- ORF length:
- 3852
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcataagaag agggttgaag agggggaggc ctcagacttc tcgctggcct 121 gggattcctc cgtgacagca gcaggaggcc tagaaggaga gccagagtgc gatcagaaaa 181 ccagccgtgc gctggaagac aggaacagcg tgacaagtca agaggagaga aatgaggatg 241 atgaagacat ggaggatgaa tcaatttaca cctgcgatca ctgtcagcag gacttcgagt 301 ctctggcaga cctgacggac caccgggccc accgctgtcc tggagatggt gatgacgacc 361 cacaactctc ctgggtggcc tcgtctccct ccagcaagga tgttgcgtca cccacgcaga 421 tgatcggaga tggttgtgac ctcggcctcg gcgaggagga agggggcacg ggcctgccat 481 acccttgcca gttctgcgac aagtccttca tccgcttgag ctacttgaag aggcacgagc 541 agatccacag cgacaagctg ccgttcaagt gcacctactg cagccgcctc ttcaagcaca 601 agaggagccg cgaccggcac atcaagctgc atacgggcga caagaagtat cactgccacg 661 agtgcgaggc agccttctcc cgcagcgacc acctcaagat ccacctgaag acccacagct 721 ccagcaagcc cttcaagtgc actgtgtgca agcgcggctt ctcctccacc agctcgctgc 781 agagccacat gcaggcccac aaaaagaaca aggagcatct ggccaagtcg gagaaggaag 841 ccaagaagga cgacttcatg tgcgactact gcgaggacac cttcagccag acggaggagc 901 tggagaagca cgtgctcacc cgccacccgc agctgtccga gaaggcggac ctgcagtgca 961 ttcactgccc tgaggtcttc gtcgacgaga acacactgct cgcccatatc caccaagccc 1021 acgccaacca gaaacacaag tgccccatgt gccctgagca gttctcctca gtggaaggtg 1081 tctactgcca cctggacagc caccggcagc ccgactccag caaccacagt gtcagtcccg 1141 accctgtact gggcagcgtg gcctccatga gcagcgccac acccgactcc agcgcctctg 1201 tggagcgtgg ctccaccccg gactccacct tgaagccgct gcgggggcag aagaagatgc 1261 gggatgacgg gcagggctgg accaaggtgg tctatagctg cccctattgt tccaagcggg 1321 actttaacag cctggccgtg ctggagatcc acctgaagac catccacgcg gacaagcccc 1381 agcagagcca cacatgtcag atctgcctgg actccatgcc caccctctac aacctcaacg 1441 agcacgttcg caagctgcac aagaaccatg cctaccctgt gatgcagttt ggcaacatct 1501 ctgccttcca ctgcaactac tgccccgaga tgttcgccga catcaatagc ctgcaggagc 1561 acatccgcgt ctcccactgc ggccccaacg ccaacccctc tgacggtaat aatgctttct 1621 tctgcaacca gtgctccatg ggtttcctta ctgagtcctc cctcaccgag cacatccagc 1681 aggcccactg cagtgtgggc agtgccaaac tagagtctcc ggtggtgcag cccacgcagt 1741 ccttcatgga ggtctattcc tgcccctact gcaccaactc ccccatcttt ggctccatcc 1801 tgaaactcac caagcacatc aaggagaacc acaagaacat tccactggcc cacagcaaga 1861 agtccaaggc cgagcagagc ccagtctcgt ccgatgtgga ggtgtcttcc ccgaagcggc 1921 agcggctctc agcaagcgcc aactccatct ccaatgggga gtatccttgt aatcaatgcg 1981 acctcaagtt ctccaacttt gagagcttcc agacccacct gaagctgcac ctggagctgc 2041 tgctgcggaa gcaagcgtgc ccccagtgca aagaggactt tgactcccag gagtccctcc 2101 tgcagcacct gacagtgcat tacatgacca cgtcgaccca ctatgtgtgc gagagctgcg 2161 acaagcaatt ttcctcggtg gatgacctgc agaagcacct gctggacatg cacacctttg 2221 tgttgtacca ctgcaccctg tgtcaggagg tcttcgactc caaggtgtcc atccaggtgc 2281 acctggcggt gaagcacagc aatgagaaga agatgtaccg ctgcacggcc tgcaactggg 2341 acttccgcaa ggaggctgac ctgcaggtgc acgtcaaaca cagccacctg ggcaacccgg 2401 ccaaggctca caagtgcatc ttctgtgggg agaccttcag caccgaggtg gagctgcagt 2461 gccacatcac cacacacagc aagaagtata actgtaagtt ctgcagcaag gccttccacg 2521 ccatcatcct gctggagaag cacctgcggg agaagcactg tgtgtttgat gctgcgaccg 2581 agaacggcac ggccaatggg gtacccccaa tggccaccaa gaaagctgag cctgctgacc 2641 tgcagggcat gctgcttaag aaccctgagg cacctaacag ccatgaggcc agcgaggatg 2701 acgtggacgc gtcggagccc atgtacggct gtgacatctg cggggcggcc tacaccatgg 2761 aggtgctgct gcagaatcac cggctgcggg accacaatat ccggccgggc gaggatgatg 2821 gctcacgcaa gaaggctgag tttatcaagg gcagtcacaa gtgcaacgtt tgttcacgga 2881 ctttcttctc ggagaacggg ctacgggagc acctgcagac gcaccggggc cctgccaagc 2941 actacatgtg tcccatctgt ggtgagcgct tcccttcgct gctgacgctc accgaacaca 3001 aggtgaccca cagcaagagc ctggacacgg gcacctgtcg catctgcaag atgcccctgc 3061 agagcgagga ggagtttatt gagcactgcc agatgcaccc tgacctgcgc aactcactca 3121 cgggcttccg ctgtgtggtc tgcatgcaga cagtcacttc cacgcttgag ctcaagatcc 3181 atggcacctt ccacatgcag aagctggcgg gcagctcagc ggcgtcctcc cccaatggcc 3241 aggggctgca gaagctctac aagtgcgccc tgtgcctcaa ggagttccgc agcaagcagg 3301 acctggtgaa gcttgacgtc aatgggctgc cctacggcct ctgcgccggc tgcatggccc 3361 gcagcgccaa cggacaggtg ggtggcctgg ccccgcccga gcccgccgac cggccctgtg 3421 ccggccTCCG TTGCCCCGAG TGCAGTGTCA AGTTTGAGAG TGCCGAAGAC CTGGAGAGCC 3481 ACATGCAGGT GGACCACCGT GACCTCACGC CGGAGACCAG TGGGCCCCGG AAAGGCACCC 3541 AGACATCGCC AGTGCCCCGG AAAAAGACAT ACCAGTGCAT CAAGTGCCAG ATGACCTTCG 3601 AGAACGAGAG AGAGATCCAA ATCCACGTTG CCAACCACAT GATTGAGGAA GGCATCAACC 3661 ACGAGTGTAA GCTGTGCAAC CAGATGTTCG ACTCCCCGGC CAAGCTCCTC TGTCACCTCA 3721 TTGAGCACAG CTTCGAGGGC ATGGGCGGCA CCTTCAAATG CCCCGTGTGT TTCACAGTCT 3781 TCGTCCAGGC CAACAAGTTG CAGCAGCACA TCTTTGCCGT GCACGGGCAG GAGGACAAGA 3841 TCTACGACTG CTCACAGTGC CCTCAGAAGT TCTTCTTCCA GACCGAGCTG CAGAACCACA 3901 CGATGAGCCA GCACGCACAG TTGCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 3961 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 4021 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAGCCT ACTCAAGCGC 4081 AAGCGAATGC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt