Construct: ORF TRCN0000473275
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014030.1_s317c1
- Derived from:
- ccsbBroadEn_12726
- DNA Barcode:
- GATGACACGCCCCAAGTCGGGAAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TSPAN14 (81619)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473275
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 81619 | TSPAN14 | tetraspanin 14 | NM_001351266.1 | 93.7% | 93.7% | 81_131del |
| 2 | human | 81619 | TSPAN14 | tetraspanin 14 | NM_001351267.3 | 93.7% | 93.7% | 81_131del |
| 3 | human | 81619 | TSPAN14 | tetraspanin 14 | NM_001351268.1 | 93.7% | 93.7% | 81_131del |
| 4 | human | 81619 | TSPAN14 | tetraspanin 14 | NM_001351269.1 | 93.7% | 93.7% | 81_131del |
| 5 | human | 81619 | TSPAN14 | tetraspanin 14 | NM_001351270.1 | 93.7% | 93.7% | 81_131del |
| 6 | human | 81619 | TSPAN14 | tetraspanin 14 | NM_001351271.1 | 93.7% | 93.7% | 81_131del |
| 7 | human | 81619 | TSPAN14 | tetraspanin 14 | NM_001351272.1 | 93.7% | 93.7% | 81_131del |
| 8 | human | 81619 | TSPAN14 | tetraspanin 14 | NM_030927.3 | 93.7% | 93.7% | 81_131del |
| 9 | human | 81619 | TSPAN14 | tetraspanin 14 | XM_017016712.2 | 93.2% | 90.1% | (many diffs) |
| 10 | human | 81619 | TSPAN14 | tetraspanin 14 | XM_017016713.2 | 93.2% | 90.1% | (many diffs) |
| 11 | human | 81619 | TSPAN14 | tetraspanin 14 | XM_011540219.3 | 89% | 89% | 81_173del |
| 12 | human | 81619 | TSPAN14 | tetraspanin 14 | XM_011540220.3 | 89% | 89% | 81_173del |
| 13 | human | 81619 | TSPAN14 | tetraspanin 14 | XM_011540221.3 | 89% | 89% | 81_173del |
| 14 | human | 81619 | TSPAN14 | tetraspanin 14 | XM_024448211.1 | 89% | 89% | 81_173del |
| 15 | human | 81619 | TSPAN14 | tetraspanin 14 | XM_024448212.1 | 89% | 89% | 81_173del |
| 16 | human | 81619 | TSPAN14 | tetraspanin 14 | NM_001128309.2 | 58.1% | 58.1% | 81_82ins318 |
| 17 | mouse | 52588 | Tspan14 | tetraspanin 14 | NM_001316748.1 | 84.4% | 91.1% | (many diffs) |
| 18 | mouse | 52588 | Tspan14 | tetraspanin 14 | NM_145928.2 | 84.4% | 91.1% | (many diffs) |
| 19 | mouse | 52588 | Tspan14 | tetraspanin 14 | XM_006519261.1 | 84.4% | 91.1% | (many diffs) |
| 20 | mouse | 52588 | Tspan14 | tetraspanin 14 | XM_017316081.1 | 72.3% | 79% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 825
- ORF length:
- 759
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca ctattataga tactctaacg ccaaggtcag ctgctggtac aagtacctcc 121 ttttcagcta caacatcatc ttctggggtg tgctgtccga cctcaccaaa gtgacccgga 181 tgcatggaat cgaccctgtg gtgctggtcc tgatggtggg cgtggtgatg ttcaccctgg 241 ggttcgccgg ctgcgtgggg gctctgcggg agaaTATCTG CTTGCTCAAC TTTTTCTGTG 301 GCACCATCGT GCTCATCTTC TTCCTGGAGC TGGCTGTGGC CGTGCTGGCC TTCCTGTTCC 361 AGGACTGGGT GAGGGACCGG TTCCGGGAGT TCTTCGAGAG CAACATCAAG TCCTACCGGG 421 ACGATATCGA TCTGCAAAAC CTCATCGACT CCCTTCAGAA AGCTAACCAG TGCTGTGGCG 481 CATATGGCCC TGAAGACTGG GACCTCAACG TCTACTTCAA TTGCAGCGGT GCCAGCTACA 541 GCCGAGAGAA GTGCGGGGTC CCCTTCTCCT GCTGCGTGCC AGATCCTGCG CAAAAAGTTG 601 TGAACACACA GTGTGGATAT GATGTCAGGA TTCAGCTGAA GAGCAAGTGG GATGAGTCCA 661 TCTTCACGAA AGGCTGCATC CAGGCGCTGG AAAGCTGGCT CCCGCGGAAC ATTTACATTG 721 TGGCTGGCGT CTTCATCGCC ATCTCGCTGT TGCAGATATT TGGCATCTTC CTGGCAAGGA 781 CGCTGATCTC AGACATCGAG GCAGTGAAGG CCGGCCATCA CTTCTCCCCA ACTTTCTTGT 841 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 901 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 961 GAAAGGACGA GATGACACGC CCCAAGTCGG GAAGACGCGT TAAGTCgaca atcaacctct 1021 ggattacaaa atttgtgaaa gatt