Construct: ORF TRCN0000473276
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005502.1_s317c1
- Derived from:
- ccsbBroadEn_08637
- DNA Barcode:
- CACATCTACTGGGTCACACAGCCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMX4 (56255)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473276
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56255 | TMX4 | thioredoxin related transme... | NM_021156.4 | 99.9% | 99.7% | 907G>A |
| 2 | mouse | 52837 | Tmx4 | thioredoxin-related transme... | NM_029148.1 | 81.1% | 80% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1113
- ORF length:
- 1047
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gggtgggcgc tgcggcccgc agctaacggc gctcctggcc gcctggatcg 121 cggctgtggc ggcgacggca ggccccgagg aggccgcgct gccgccggag cagagccggg 181 tccagcccat gaccgcctcc aactggacgc tggtgatgga gggcgagtgg atgctgaaat 241 tttacgcccc atggtgtcca tcctgccagc agactgattc agaatgggag gcttttgcaa 301 agaatggtga aatacttcag atcagtgtgg ggaaggtaga tgtcattcaa gaaccaggtt 361 tgagtggccg cttctttgtc accactctcc cagcattttt tcatgcaaag gatgggatat 421 tccgccgtta tcgtggccca ggaatcttcg aagacctgca gaattatatc ttagagaaga 481 aatggcaatc agtcgagcct ctgactggct ggaaatcccc ggcttctcta acgatgtctg 541 gaatggctgg tctttttagc atctctggca agatatggca tcttcacaac tatttcacag 601 tgactcttgg aattcctgct tggtgttctt atgtcttttt cgtcatagcc accttggttt 661 ttggCCTTTT TATGGGTCTG GTCTTGGTGG TAATATCAGA ATGTTTCTAT GTGCCACTTC 721 CAAGGCATTT ATCTGAGCGT TCTGAGCAGA ATCGGAGATC AGAGGAGGCT CATAGAGCTG 781 AACAGTTGCA GGATGCGGAG GAGGAAAAAG ATGATTCAAA TGAAGAAGAA AACAAAGACA 841 GCCTTGTAGA TGATGAAGAA GAGAAAGAAG ATCTTGGCGA TGAGGATGAA GCAGAGGAAG 901 AAGAGGAGGA GGACAACTTG GCTGCTGGTG TGGATGAGGA GAGAAGTGAG GCCAATGATC 961 AGGGGCCCCC AAGAGAGGAC GGTGTGACCC GGGAGGAAGT AGAGCCTGAG GAGGCTGAAG 1021 AAGGCATCTC TGAGCAACCC TGCCCAGCTG ACACAGAGGT GGTGGAAGAC TCCTTGAGGC 1081 AGCGTAAAAG TCAGCATGCT GACAAGGGAC TGTACCCAAC TTTCTTGTAC AAAGTGGTTG 1141 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1201 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACA 1261 CATCTACTGG GTCACACAGC CTACGCGTTA AGTCgacaat caacctctgg attacaaaat 1321 ttgtgaaaga tt