Construct: ORF TRCN0000473288
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018993.3_s317c1
- Derived from:
- ccsbBroadEn_14664
- DNA Barcode:
- CAGAATGGGGCTAGTTGGCACACT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HCK (3055)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473288
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3055 | HCK | HCK proto-oncogene, Src fam... | NM_002110.4 | 100% | 96% | |
| 2 | human | 3055 | HCK | HCK proto-oncogene, Src fam... | NM_001172130.2 | 99.8% | 95.8% | 225_226insGCA |
| 3 | human | 3055 | HCK | HCK proto-oncogene, Src fam... | NM_001172132.2 | 96.1% | 99.8% | 0_1ins60;2T>G |
| 4 | human | 3055 | HCK | HCK proto-oncogene, Src fam... | NM_001172129.2 | 96% | 100% | 0_1ins63 |
| 5 | human | 3055 | HCK | HCK proto-oncogene, Src fam... | NM_001172133.2 | 96% | 100% | 0_1ins63 |
| 6 | human | 3055 | HCK | HCK proto-oncogene, Src fam... | NM_001172131.2 | 95.8% | 99.8% | 0_1ins63;162_163insGCA |
| 7 | mouse | 15162 | Hck | hemopoietic cell kinase | NM_010407.4 | 86.2% | 86.1% | (many diffs) |
| 8 | mouse | 15162 | Hck | hemopoietic cell kinase | NM_001172117.1 | 82.7% | 89.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 132
- ORF end:
- 1647
- ORF length:
- 1515
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccct gggggggcgc tcaagctgcg aggatccggg ctgcccgcga gacgaggagc 121 gggcgcccag gatggggtgc atgaagtcca agttcctcca ggtcggaggc aatacattct 181 caaaaactga aaccagcgcc agcccacact gtcctgtgta cgtgccggat cccacatcca 241 ccatcaagcc ggggcctaat agccacaaca gcaacacacc aggaatcagg gaggcaggct 301 ctgaggacat catcgtggtt gccctgtatg attacgaggc cattcaccac gaagacctca 361 gcttccagaa gggggaccag atggtggtcc tagaggaatc cggggagtgg tggaaggctc 421 gatccctggc cacccggaag gagggctaca tcccaagcaa ctatgtcgcc cgcgttgact 481 ctctggagac agaggagtgg tttttcaagg gcatcagccg gaaggacgca gagcgccaac 541 tgctggctcc cggcaacatg ctgggctcct tcatgatccg ggatagcgag accactaaag 601 gaagctactc tttgtccgtg cgagactacg accctcggca gggagatacc gtgaaacatt 661 acaagatccg gaccctggac aacgggggct tctacatatc cccccgaagc accttcagca 721 ctctgcagga gctggtggac cactacaaga aggggaacga cgggctctgc cagaaactgt 781 cggtgccctg catgtcttcc aagccccaga agccttggga gaaagatgcc tgggagatcc 841 ctcgggaatc cctcaagctg gagaagaaac ttggagctgg gcagtttggg gaagtctgga 901 tggccaccta caacaagcac accaaggtgg cagtgaagac gatgaagcca gggagcatgt 961 cggtggaggc cttcctggca gaggccaacg tgatgaaaac tctgcagcat gacaagctgg 1021 tcaaacttca tgcggtggtc accaaggagc ccatctacat catcacggag ttcatggcca 1081 aaggaagctt gctggacttt ctgaaaagtg atgagggcag caagcagcca ttgccaaaac 1141 tcattgactt ctcagcccag attgcagaag gcatggcctt catcgagcag aggaactaca 1201 tccaccgaga cctccgagct gccaacatct tggtcTCTGC ATCCCTGGTG TGTAAGATTG 1261 CTGACTTTGG CCTGGCCCGG GTCATTGAGG ACAACGAGTA CACGGCTCGG GAAGGGGCCA 1321 AGTTCCCCAT CAAGTGGACA GCTCCTGAAG CCATCAACTT TGGCTCCTTC ACCATCAAGT 1381 CAGACGTCTG GTCCTTTGGT ATCCTGCTGA TGGAGATCGT CACCTACGGC CGGATCCCTT 1441 ACCCAGGGAT GTCAAACCCT GAAGTGATCC GAGCTCTGGA GCGTGGATAC CGGATGCCTC 1501 GCCCAGAGAA CTGCCCAGAG GAGCTCTACA ACATCATGAT GCGCTGCTGG AAAAACCGTC 1561 CGGAGGAGCG GCCGACCTTC GAATACATCC AGAGTGTGCT GGATGACTTC TACACGGCCA 1621 CAGAGAGCCA GTACCAACAG CAGCCACCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1681 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1741 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAC AGAATGGGGC 1801 TAGTTGGCAC ACTACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1861 att