Construct: ORF TRCN0000473295
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013667.1_s317c1
- Derived from:
- ccsbBroadEn_01349
- DNA Barcode:
- GTGGCACATAAACTATTTCCTATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NECTIN1 (5818)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473295
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5818 | NECTIN1 | nectin cell adhesion molecu... | NM_002855.5 | 100% | 100% | |
| 2 | human | 5818 | NECTIN1 | nectin cell adhesion molecu... | NM_203285.2 | 76.5% | 70.3% | (many diffs) |
| 3 | human | 5818 | NECTIN1 | nectin cell adhesion molecu... | NM_203286.2 | 65.7% | 65.3% | (many diffs) |
| 4 | mouse | 58235 | Nectin1 | nectin cell adhesion molecu... | NM_021424.2 | 90.2% | 94.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1620
- ORF length:
- 1551
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggctcggatg gggcttgcgg gcgccgctgg acgctggtgg ggactcgctc 121 tcggcttgac cgcattcttc ctcccaggcg tccactccca ggtggtccag gtgaacgact 181 ccatgtatgg cttcatcggc acagacgtgg ttctgcactg cagctttgcc aacccgcttc 241 ccagcgtgaa gatcacccag gtcacatggc agaagtccac caatggctcc aagcagaacg 301 tggccatcta caacccatcc atgggcgtgt ccgtgctggc tccctaccgc gagcgtgtgg 361 aattcctgcg gccctccttc accgatggca ctatccgcct ctcccgcctg gagctggagg 421 atgagggtgt ctacatctgc gagtttgcta ccttccctac gggcaatcga gaaagccagc 481 tcaatctcac ggtgatggcc aaacccacca attggataga gggtacccag gcagtgcttc 541 gagccaagaa ggggcaggat gacaaggtcc tggtggccac ctgcacctca gccaatggga 601 agcctcccag tgtggtatcc tgggaaactc ggttaaaagg tgaggcagag taccaggaga 661 tccggaaccc caatggcaca gtgacggtca tcagccgcta ccgcctggtg cccagcaggg 721 aagcccacca gcagtccttg gcctgcatcg tcaactacca catggaccgc ttcaaggaaa 781 gcctcactct caacgtgcag tatgagcctg aggtaaccat tgaggggttt gatggcaact 841 ggtacctgca gcggatggac gtgaagctca cctgcaaagc tgatgctaac cccccagcca 901 ctgagtacca ctggaccacg ctaaatggct ctctccccaa gggtgtggag gcccagaaca 961 gaaccctctt cttcaaggga cccatcaact acagcctggc agggacctac atctgtgagg 1021 ccaccaaccc catcggtaca cgctcaggcc aggtggaggt caatatcaca gaattcccct 1081 acaccccgtc tcctcccgaa catgggcggc gcgccgggcc ggtgcccacg gccatcattg 1141 ggggcgtggc ggggagcatc ctgctggtgt tgattgtggt cggcgggatc gtggtcgccc 1201 tgcgtcggcg ccggcacacc ttcaagggtg actacagcac caagaagcac gtgtatggca 1261 acggctacag caaggcaggc atcccccagc accacccacc aatggcacag aacctgcagt 1321 accccgacga ctcagacgac gagaagaagg ccggcccact gggtggaagc agctatgagg 1381 aggaggagga ggaggaggag ggcggtggag gGGGCGAGCG CAAGGTGGGC GGCCCCCACC 1441 CCAAATATGA CGAGGACGCC AAGCGGCCCT ACTTCACCGT GGATGAGGCC GAGGCCCGTC 1501 AGGACGGCTA CGGGGACCGG ACTCTGGGCT ACCAGTACGA CCCTGAGCAG CTGGACTTGG 1561 CTGAGAACAT GGTTTCTCAG AACGACGGGT CTTTCATTTC CAAGAAGGAG TGGTACGTGT 1621 TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1681 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1741 TTTATATATC TTGTGGAAAG GACGAGTGGC ACATAAACTA TTTCCTATCA CGCGTTAAGT 1801 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt