Construct: ORF TRCN0000473296
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011217.1_s317c1
- Derived from:
- ccsbBroadEn_06474
- DNA Barcode:
- TAAAAAATTATTCTTAATCTGTCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KCNC1 (3746)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473296
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3746 | KCNC1 | potassium voltage-gated cha... | NM_004976.4 | 99.9% | 99.8% | 64T>C |
| 2 | human | 3746 | KCNC1 | potassium voltage-gated cha... | NM_001112741.2 | 86.3% | 85.8% | (many diffs) |
| 3 | human | 3746 | KCNC1 | potassium voltage-gated cha... | XR_930866.2 | 41.8% | 1_1193del;1257T>C;2727_3661del | |
| 4 | mouse | 16502 | Kcnc1 | potassium voltage gated cha... | NM_008421.3 | 93.6% | 99.6% | (many diffs) |
| 5 | mouse | 16502 | Kcnc1 | potassium voltage gated cha... | NM_001112739.2 | 80.8% | 85.6% | (many diffs) |
| 6 | mouse | 16502 | Kcnc1 | potassium voltage gated cha... | XM_006540648.1 | 80.5% | 85.3% | (many diffs) |
| 7 | mouse | 16502 | Kcnc1 | potassium voltage gated cha... | XM_006540647.1 | 79.5% | 84.6% | (many diffs) |
| 8 | mouse | 16502 | Kcnc1 | potassium voltage gated cha... | XM_006540646.1 | 79.2% | 84.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1602
- ORF length:
- 1533
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gggccaaggg gacgagagcg agcgcatcgt gatcaacgtg ggcggcacgc 121 gccaccagac gcaccgctcg accctgcgca cgctgcccgg cacgcggctc gcctggctgg 181 cggagcccga cgcccacagc cacttcgact atgacccgcg tgctgacgag ttcttcttcg 241 accgccaccc cggcgtcttc gcgcacatcc tgaactacta ccgcacgggc aagctgcact 301 gcccagccga cgtgtgcggg ccgctctacg aggaggagct ggccttctgg ggcatcgacg 361 agaccgacgt ggagccctgc tgctggatga cgtaccgcca gcaccgcgac gccgaggagg 421 ctctggacag cttcggcggc gctcctctgg acaacagcgc cgacgacgcg gacgccgacg 481 gccctggcga ctcgggcgac ggcgaggacg agctggagat gaccaagcgc ctggcgctca 541 gtgactcccc ggatggccgg cctggcggct tttggcgccg ctggcagccg cgcatctggg 601 cgctcttcga ggacccgtac tcgtcccgct acgcgcggta tgtggccttc gcttccctct 661 tcttcatcct ggtctccatc accaccttct gcctggagac ccacgagcgc ttcaacccca 721 tcgtgaacaa gacggagatc gagaacgttc gcaatggcac gcaagtgcgc tactaccggg 781 aggccgagac ggaggccttc cttacctaca tcgagggcgt ctgtgtggtc tggttcacct 841 tcgagttcct catgcgtgtc atcttctgcc ccaacaaggt agagttcatc aagaactcgc 901 tcaacatcat tgactttgtg gccatcctgc ccttctacct ggaggtgggg ctgagcggcc 961 tgtcctccaa ggcagccaag gacgtgctgg gcttcctgcg cgtcgtccgc ttcgtgcgca 1021 tcttgcgcat ctttaagctg acccgccact ttgtgggcct gcgggtcctg ggccacacgc 1081 tccgagccag caccaacgag ttcctgctgc tcatcatctt cctggccTTG GGCGTGCTGA 1141 TCTTCGCCAC CATGATCTAC TACGCCGAGA GGATAGGGGC ACAGCCCAAT GACCCCAGCG 1201 CCAGTGAGCA CACGCACTTT AAGAACATCC CCATCGGCTT CTGGTGGGCC GTGGTCACCA 1261 TGACGACCCT GGGCTATGGA GACATGTACC CGCAGACGTG GTCCGGCATG CTGGTGGGGG 1321 CTCTGTGTGC GCTGGCGGGC GTGCTCACCA TCGCCATGCC CGTGCCCGTC ATCGTGAACA 1381 ATTTCGGGAT GTATTACTCC TTAGCCATGG CTAAGCAGAA ACTACCAAAG AAAAAAAAGA 1441 AGCATATTCC GCGGCCACCG CAGCTGGGAT CTCCCAATTA TTGTAAATCT GTCGTAAACT 1501 CTCCACACCA CAGTACTCAG AGTGACACAT GTCCGCTGGC CCAGGAAGAA ATTTTAGAAA 1561 TTAACAGAGC AGGTAGGAAA CCTCTTAGAG GCATGTCGAT CTTGCCAACT TTCTTGTACA 1621 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1681 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1741 AGGACGATAA AAAATTATTC TTAATCTGTC AACGCGTTAA GTCgacaatc aacctctgga 1801 ttacaaaatt tgtgaaagat t