Construct: ORF TRCN0000473317
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004233.1_s317c1
- Derived from:
- ccsbBroadEn_15398
- DNA Barcode:
- TAAGATGAATGGCACCACTCGTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DKC1 (1736)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473317
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1736 | DKC1 | dyskerin pseudouridine synt... | NM_001363.5 | 99.8% | 99.8% | 369G>T;853G>T |
| 2 | human | 1736 | DKC1 | dyskerin pseudouridine synt... | NM_001142463.3 | 98.8% | 98.6% | 369G>T;853G>T;1244_1245insGTATGTTGACTACAG |
| 3 | human | 1736 | DKC1 | dyskerin pseudouridine synt... | NM_001288747.1 | 81.5% | 81.3% | 853G>T;1260_1260delGins283 |
| 4 | human | 1736 | DKC1 | dyskerin pseudouridine synt... | NR_110023.2 | 54.2% | (many diffs) | |
| 5 | human | 1736 | DKC1 | dyskerin pseudouridine synt... | NR_110021.2 | 52.2% | (many diffs) | |
| 6 | human | 1736 | DKC1 | dyskerin pseudouridine synt... | NR_110022.2 | 50.2% | (many diffs) | |
| 7 | mouse | 245474 | Dkc1 | dyskeratosis congenita 1, d... | NM_001030307.2 | 87.9% | 90.8% | (many diffs) |
| 8 | mouse | 245474 | Dkc1 | dyskeratosis congenita 1, d... | XM_006528023.3 | 83.4% | 85.5% | (many diffs) |
| 9 | mouse | 245474 | Dkc1 | dyskeratosis congenita 1, d... | XM_017318488.1 | 51.9% | 53.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1608
- ORF length:
- 1542
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggatgcggaa gtaattattt tgccaaagaa acataagaag aaaaaggagc 121 ggaagtcatt gccagaagaa gatgtagccg aaatacaaca cgctgaagaa tttcttatca 181 aacctgaatc caaagttgct aagttggaca cgtctcagtg gccccttttg ctaaagaatt 241 ttgataagct gaatgtaagg acaacacact atacacctct tgcatgtggt tcaaatcctc 301 tgaagagaga gattggggac tatatcagga caggtttcat taatcttgac aagccctcta 361 acccctcttc ccatgaggtg gtagcctgga ttcgacggat acttcgggtg gagaagacag 421 ggcacagtgg tactctggat cccaaggtga ctggttgttt aatcgtgtgc atagaacgag 481 ccactcgctt ggtgaagtca caacagagtg caggcaaaga gtatgtgggg attgtccggc 541 tgcacaatgc tattgaaggg gggacccagc tttctagggc cctagaaact ctgacaggtg 601 ccttattcca gcgaccccca cttattgctg cagtaaagag gcagctccga gtgaggacca 661 tctacgagag caaaatgatt gaatacgatc ctgaaagaag attaggaatc ttttgggtga 721 gttgtgaggc tggcacctac attcggacat tatgtgtgca ccttggtttg ttattgggag 781 ttggtggtca gatgcaggag cttcggaggg ttcgttctgg agtcatgagt gaaaaggacc 841 acatggtgac aatgcatgat gtgcttgatg ctcagtggct gtatgataac cacaaggatg 901 agagttacct gcggcgattt gtttaccctt tggaaaagct gttgacatct cataaacggc 961 tggttatgaa agacagtgca gtaaatgcca tctgctatgg ggccaagatt atgcttccag 1021 gtgttcttcg atatgaggac ggcattgagg tcaatcagga gattgtggtt atcaccacca 1081 aaggagaagc aatctgcatg gctattgcat taatgaccac agcggTCATC TCTACCTGCG 1141 ACCATGGTAT AGTAGCCAAG ATCAAGAGAG TGATCATGGA GAGAGACACT TACCCTCGGA 1201 AGTGGGGTTT AGGTCCAAAG GCAAGTCAGA AGAAGCTGAT GATCAAGCAG GGCCTTCTGG 1261 ACAAGCATGG GAAGCCCACA GACAGCACAC CTGCCACCTG GAAGCAGGAG TATGTTGACT 1321 ACAGTGAGTC TGCCAAAAAA GAGGTGGTTG CTGAAGTGGT AAAAGCCCCG CAGGTAGTTG 1381 CCGAAGCAGC AAAAACTGCG AAGCGGAAGC GAGAGAGTGA GAGTGAAAGT GACGAGACTC 1441 CTCCAGCAGC TCCTCAGTTG ATCAAGAAGG AAAAGAAGAA GAGTAAGAAG GACAAGAAGG 1501 CCAAAGCTGG TCTGGAGAGC GGGGCCGAGC CTGGAGATGG GGACAGTGAT ACCACCAAGA 1561 AGAAGAAGAA GAAGAAGAAA GCAAAAGAGG TAGAATTGGT TTCTGAGTAC CCAACTTTCT 1621 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1681 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1741 GTGGAAAGGA CGATAAGATG AATGGCACCA CTCGTTCACG CGTTAAGTCg acaatcaacc 1801 tctggattac aaaatttgtg aaagatt