Construct: ORF TRCN0000473319
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000963.1_s317c1
- Derived from:
- ccsbBroadEn_03278
- DNA Barcode:
- GGGCTCAACACTTCGCTGTGCTGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FAM53C (51307)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473319
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51307 | FAM53C | family with sequence simila... | NM_001135647.2 | 100% | 100% | |
| 2 | human | 51307 | FAM53C | family with sequence simila... | NM_016605.3 | 100% | 100% | |
| 3 | human | 51307 | FAM53C | family with sequence simila... | NM_001350195.2 | 95.7% | 93.9% | (many diffs) |
| 4 | human | 51307 | FAM53C | family with sequence simila... | XM_024446108.1 | 89.1% | 85.8% | (many diffs) |
| 5 | human | 51307 | FAM53C | family with sequence simila... | NM_001350194.2 | 46.1% | 46.1% | 135_136ins633 |
| 6 | mouse | 66306 | Fam53c | family with sequence simila... | NM_175104.4 | 92.8% | 94.4% | (many diffs) |
| 7 | mouse | 66306 | Fam53c | family with sequence simila... | XM_006526151.3 | 92.8% | 94.4% | (many diffs) |
| 8 | mouse | 66306 | Fam53c | family with sequence simila... | XM_006526152.2 | 92.8% | 94.4% | (many diffs) |
| 9 | mouse | 66306 | Fam53c | family with sequence simila... | XM_006526153.3 | 84.1% | 84.4% | (many diffs) |
| 10 | mouse | 66306 | Fam53c | family with sequence simila... | XM_006526154.3 | 84.1% | 84.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1242
- ORF length:
- 1176
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat aaccctgatc actgagcagc tacagaagca gactctggat gagctgaaat 121 gcacacgctt cagcatcagt ctgcctttgc ctgatcatgc agacatctcc aactgtggga 181 actctttcca gcttgtgtct gaaggtgctt cctggagggg cctgccccac tgttcctgtg 241 ctgagttcca ggacagcctc aacttcagct accatccctc aggcctgagc ctgcacctca 301 gaccacccag tcggggaaac tcccccaagg agcagccctt ctcccaagtc ctaagacctg 361 agcccccaga tccagagaag cttcctgtgc cccctgcccc tccatccaag aggcactgcc 421 gctcactctc agtgcccgtg gacctgtctc gctggcagcc ggtgtggcgg cccgccccct 481 ccaagctgtg gactcccata aagcaccggg gcagtggtgg agggggtggg ccgcaggtgc 541 ctcaccagag ccccccaaag cgggtctcca gcctcaggtt cctccaagct cccagtgcct 601 cttctcaatg tgccccagct cacagaccct acagccctcc tttcttcagc ctggccctgg 661 cccaagattc ctctcgaccc tgcgccgcct cccctcaaag tggctcctgg gagagtgatg 721 ctgagtcctt gtcaccttgc ccacctcagc gccgcttctc cctgtcaccc agtctgggcc 781 cgcaggcaag ccgcttcttg cccTCTGCCC GGAGCTCTCC CGCATCCTCC CCAGAGCTGC 841 CCTGGCGACC TCGAGGTCTC CGCAACCTTC CCCGAAGCCG CTCACAGCCT TGTGATCTGG 901 ATGCCCGCAA AACTGGGGTC AAGCGGCGCC ACGAGGAAGA CCCCCGGCGT CTGCGGCCTT 961 CGTTGGACTT TGACAAGATG AATCAGAAAC CATACTCAGG AGGTCTTTGT CTCCAAGAAA 1021 CAGCCCGGGA AGGCAGCAGC ATCTCTCCAC CATGGTTCAT GGCCTGTAGC CCCCCACCCC 1081 TCTCTGCTTC CTGCAGCCCC ACTGGGGGTT CCTCCCAGGT GCTGAGTGAA AGCGAAGAGG 1141 AGGAGGAGGG GGCTGTGCGG TGGGGTCGGC AGGCGCTGAG CAAGCGGACA CTGTGCCAGC 1201 GGGACTTTGG GGACCTGGAC TTGAATTTGA TTGAGGAAAA CTACCCAACT TTCTTGTACA 1261 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1321 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1381 AGGACGAGGG CTCAACACTT CGCTGTGCTG TACGCGTTAA GTCgacaatc aacctctgga 1441 ttacaaaatt tgtgaaagat t