Construct: ORF TRCN0000473332

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009427.1_s317c1
Derived from:
ccsbBroadEn_01050
DNA Barcode:
TGACTATTTCTACATTCCTGGACA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MUC1 (4582)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000473332
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 4582 MUC1 mucin 1, cell surface assoc... NM_001018016.3 100% 100%
2 human 4582 MUC1 mucin 1, cell surface assoc... NM_001018017.3 96.5% 96.5% 54_55ins27
3 human 4582 MUC1 mucin 1, cell surface assoc... NM_001204287.2 93.6% 93.6% 187_240del
4 human 4582 MUC1 mucin 1, cell surface assoc... NM_001204291.1 91.2% 91.2% 186_187ins69
5 human 4582 MUC1 mucin 1, cell surface assoc... NM_001204292.1 90.5% 85.9% 186_187insTTTAATTCCTCTCTGGAAG;235_236ins56
6 human 4582 MUC1 mucin 1, cell surface assoc... NM_002456.6 90.4% 90.4% 54_55ins27;160_213del
7 human 4582 MUC1 mucin 1, cell surface assoc... NM_001204289.2 90.1% 90.1% 186_187ins78
8 human 4582 MUC1 mucin 1, cell surface assoc... NM_001204294.2 87.1% 82.5% 54_55ins27;159_160insTTTAATTCCTCTCTGGAAG;208_209ins56
9 human 4582 MUC1 mucin 1, cell surface assoc... NM_001204288.2 82.9% 59.9% 447_448ins32;657_658ins103
10 human 4582 MUC1 mucin 1, cell surface assoc... NM_001204290.2 82.1% 82.1% 55_56ins63;123_124ins78
11 human 4582 MUC1 mucin 1, cell surface assoc... NM_001204296.2 80.3% 69.3% 186_187insTTTAATTCCTCTCTGGAAG;291_292ins137
12 human 4582 MUC1 mucin 1, cell surface assoc... NM_001044390.3 76.8% 65.9% 54_55ins27;159_160insTTTAATTCCTCTCTGGAAG;264_265ins137
13 human 4582 MUC1 mucin 1, cell surface assoc... NM_001204295.1 71.5% 71.5% 254_255ins225
14 human 4582 MUC1 mucin 1, cell surface assoc... NM_001204293.2 63.8% 48.9% (many diffs)
15 human 4582 MUC1 mucin 1, cell surface assoc... NM_001044392.3 60.2% 60.2% 253_254ins315
16 human 4582 MUC1 mucin 1, cell surface assoc... NM_001044393.3 59.8% 25.8% (many diffs)
17 human 4582 MUC1 mucin 1, cell surface assoc... NM_001044391.3 56.8% 56.8% 54_55ins27;226_227ins315
18 human 4582 MUC1 mucin 1, cell surface assoc... NM_001204297.2 56.3% 56.3% 187_240del;307_308ins315
19 human 4582 MUC1 mucin 1, cell surface assoc... NM_001204286.1 54.5% 54.5% 187_846del
20 human 4582 MUC1 mucin 1, cell surface assoc... NM_001204285.2 52.6% 52.6% 54_55ins27;160_819del
21 human 4582 MUC1 mucin 1, cell surface assoc... NM_001371720.1 19.8% 17.7% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
861
ORF length:
792
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gacaccgggc acccagtctc ctttcttcct gctgctgctc ctcacagtgc
121ttacagctac cacagcccct aaacccgcaa cagttgttac gggttctggt catgcaagct
181ctaccccagg tggagaaaag gagacttcgg ctacccagag aagttcagtg cccagctcta
241ctgagaagaa tgcttttaat tcctctctgg aagatcccag caccgactac taccaagagc
301tgcagagaga catttctgaa atgtttttgc agatttataa acaagggggt tttctgggcc
361tctccaatat taagttcagg ccaggatctg tggtggtaca attgactctg gccttccgag
421aaggtaccat caatgtccac gacgtggaga cacagttcaa tcagtataaa acggaagcag
481cctctcgata taaCCTGACG ATCTCAGACG TCAGCGTGAG TGATGTGCCA TTTCCTTTCT
541CTGCCCAGTC TGGGGCTGGG GTGCCAGGCT GGGGCATCGC GCTGCTGGTG CTGGTCTGTG
601TTCTGGTTGC GCTGGCCATT GTCTATCTCA TTGCCTTGGC TGTCTGTCAG TGCCGCCGAA
661AGAACTACGG GCAGCTGGAC ATCTTTCCAG CCCGGGATAC CTACCATCCT ATGAGCGAGT
721ACCCCACCTA CCACACCCAT GGGCGCTATG TGCCCCCTAG CAGTACCGAT CGTAGCCCCT
781ATGAGAAGGT TTCTGCAGGT AATGGTGGCA GCAGCCTCTC TTACACAAAC CCAGCAGTGG
841CAGCCACTTC TGCCAACTTG TTGCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC
901CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG
961AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATGAC TATTTCTACA
1021TTCCTGGACA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)