Construct: ORF TRCN0000473398
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010755.1_s317c1
- Derived from:
- ccsbBroadEn_04527
- DNA Barcode:
- TTAAAAGTCTATCAGTCGCAGGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SEC11C (90701)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473398
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 90701 | SEC11C | SEC11 homolog C, signal pep... | NM_033280.4 | 100% | 100% | |
| 2 | human | 90701 | SEC11C | SEC11 homolog C, signal pep... | XM_011526260.3 | 87.6% | 84.7% | (many diffs) |
| 3 | human | 90701 | SEC11C | SEC11 homolog C, signal pep... | NM_001307941.2 | 83.8% | 81.7% | 466_467ins58;483_484ins35 |
| 4 | human | 90701 | SEC11C | SEC11 homolog C, signal pep... | XM_017026073.1 | 80.2% | 80.2% | 0_1ins114 |
| 5 | mouse | 66286 | Sec11c | SEC11 homolog C, signal pep... | NM_025468.2 | 92.1% | 97.9% | (many diffs) |
| 6 | mouse | 66286 | Sec11c | SEC11 homolog C, signal pep... | XM_006526150.1 | 74.6% | 80.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 642
- ORF length:
- 576
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt gcgtgcgggc gccgtggggg ctcatctccc cgcgtccggc ttggatatct 121 tcggggacct gaagaagatg aacaagcgcc agctctatta ccaggtttta aacttcgcca 181 tgatcgtgtc ttctgcactc atgatatgga aaggcttgat cgtgctcaca ggcagtgaga 241 gccccatcgt ggtggtgctg agtggcagta tggagccggc ctttcacaga ggagacctcc 301 tgttcctcac aaatttccgg gaagacccaa tcagagctgg tgaaatagtt gtttttaaag 361 ttgaaggacg agacattcca atagttcaca gagtaatcaa agttcatgaa aaagataatg 421 gagacatcaa atttctgact aaaggagata ataatgaagt tGATGATAGA GGCTTGTACA 481 AAGAAGGCCA GAACTGGCTG GAAAAGAAGG ACGTGGTGGG AAGAGCAAGA GGGTTTTTAC 541 CATATGTTGG TATGGTCACC ATAATAATGA ATGACTATCC AAAATTCAAG TATGCTCTTT 601 TGGCTGTAAT GGGTGCATAT GTGTTACTAA AACGTGAATC CTACCCAACT TTCTTGTACA 661 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 721 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 781 AGGACGATTA AAAGTCTATC AGTCGCAGGC AACGCGTTAA GTCgacaatc aacctctgga 841 ttacaaaatt tgtgaaagat t