Construct: ORF TRCN0000473405
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018999.1_s317c1
- Derived from:
- ccsbBroadEn_14859
- DNA Barcode:
- CGGCTAAAATTGACTCAAGCCGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TEC (7006)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473405
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7006 | TEC | tec protein tyrosine kinase | NM_003215.3 | 100% | 100% | |
| 2 | human | 7006 | TEC | tec protein tyrosine kinase | XM_024454193.1 | 100% | 100% | |
| 3 | human | 7006 | TEC | tec protein tyrosine kinase | XM_011513737.2 | 94.1% | 92.8% | (many diffs) |
| 4 | human | 7006 | TEC | tec protein tyrosine kinase | XM_017008569.1 | 81.6% | 81.6% | 0_1ins348 |
| 5 | human | 7006 | TEC | tec protein tyrosine kinase | XM_011513741.1 | 73.8% | 73% | 1374_1375ins95;1398_1399ins400 |
| 6 | human | 7006 | TEC | tec protein tyrosine kinase | XR_001741318.1 | 51.1% | 1_45del;1514_1515ins65;1874_3509del | |
| 7 | human | 7006 | TEC | tec protein tyrosine kinase | XR_925160.2 | 48.4% | 1_45del;1418_1419ins160;1779_3414del | |
| 8 | human | 7006 | TEC | tec protein tyrosine kinase | XR_001741319.1 | 45.4% | 1_158del;1411_1412ins217;1835_3470del | |
| 9 | mouse | 21682 | Tec | tec protein tyrosine kinase | NM_001113460.2 | 88.9% | 95% | (many diffs) |
| 10 | mouse | 21682 | Tec | tec protein tyrosine kinase | NM_001113464.2 | 88.9% | 95% | (many diffs) |
| 11 | mouse | 21682 | Tec | tec protein tyrosine kinase | XM_006503845.3 | 88.9% | 95% | (many diffs) |
| 12 | mouse | 21682 | Tec | tec protein tyrosine kinase | XM_006503846.3 | 88.9% | 95% | (many diffs) |
| 13 | mouse | 21682 | Tec | tec protein tyrosine kinase | XM_011240718.2 | 88.9% | 95% | (many diffs) |
| 14 | mouse | 21682 | Tec | tec protein tyrosine kinase | XM_011240719.2 | 88.9% | 95% | (many diffs) |
| 15 | mouse | 21682 | Tec | tec protein tyrosine kinase | NM_001113461.2 | 85.7% | 91.7% | (many diffs) |
| 16 | mouse | 21682 | Tec | tec protein tyrosine kinase | XM_006503847.2 | 84% | 88.2% | (many diffs) |
| 17 | mouse | 21682 | Tec | tec protein tyrosine kinase | XM_006503848.3 | 77.2% | 81.2% | (many diffs) |
| 18 | mouse | 21682 | Tec | tec protein tyrosine kinase | NM_013689.5 | 74% | 77.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1962
- ORF length:
- 1893
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaattttaac actattttgg aggagattct tattaaaagg tcacagcaga 121 aaaagaagac atcgccctta aactacaaag agagactttt tgtacttaca aagtccatgc 181 taacctacta tgagggtcga gcagagaaga aatacagaaa ggggtttatt gatgtttcaa 241 aaatcaagtg tgtggaaata gtgaagaatg atgatggtgt cattccctgt caaaataagt 301 atccatttca ggttgttcat gatgctaaca cactttacat ttttgcacct agtccacaaa 361 gcagggacct gtgggtgaag aagttaaaag aagaaataaa gaacaacaat aatattatga 421 ttaaatatca tcctaaattc tggacagatg gaagttatca gtgttgtaga caaactgaaa 481 aattagcacc cggatgtgaa aaatacaatc tttttgagag cagtataaga aaagcactac 541 ctccagcacc agaaacaaag aagcgaaggc ctcccccacc aattccacta gaagaagaag 601 ataatagtga agaaatcgtt gtagccatgt atgatttcca agcagcagaa ggacatgatc 661 tcagattaga gagaggccaa gagtatctca ttttagaaaa gaatgatgtt cattggtgga 721 gagcaagaga taaatatggg aatgaaggat atatcccaag taattacgta acgggaaaga 781 aatcaaacaa cttagatcaa tatgaatggt attgcagaaa tatgaataga agcaaggcag 841 agcaactcct ccgcagtgaa gataaagaag gtggttttat ggtaagggat tccagtcaac 901 caggcttgta cacagtctcc ctttatacca agtttggagg agaaggttca tcgggtttta 961 ggcattatca tataaaggaa acaacaacat ctccaaagaa gtattaccta gctgaaaaac 1021 atgcttttgg ctccattcct gagattattg aatatcataa gcacaatgca gcaggacttg 1081 tcaccaggct tcggtaccca gttagtgtga aagggaagaa tgcacccacc actgcaggat 1141 tcagctatga gaaatgggag attaaccctt cagaactgac ctttatgagg gaattgggaa 1201 gtggactgtt tggagtggtg aggcttggca aatggcgagc ccagtacaaa gtcgcaatca 1261 aagctattcg ggaaggtgca atgtgcgagg aggactttat agaagaagct aaagtgatga 1321 tgaaactgac acacccgaag ttagtgcagc tttatggtgt gtgcacccag cagaaaccaa 1381 tatacattgt tactgagttc atggaaaggg gctgccttct gaatttcctc cgacagagac 1441 aaggtcattt cagtagagac gtactgctga gcatgtgtca ggatgtgtgt gaagggatgg 1501 agtatcTGGA GAGAAACAGC TTCATCCACA GAGATCTGGC TGCCAGAAAT TGTCTAGTAA 1561 GTGAGGCGGG AGTTGTAAAA GTATCTGATT TTGGAATGGC CAGGTATGTT CTGGATGATC 1621 AGTACACAAG TTCTTCTGGT GCTAAGTTTC CTGTGAAGTG GTGTCCACCT GAAGTGTTTA 1681 ATTACAGCCG CTTCAGCAGC AAATCAGATG TCTGGTCATT TGGTGTTTTA ATGTGGGAAG 1741 TATTCACGGA AGGCAGAATG CCTTTTGAAA AATACACCAA TTATGAAGTG GTAACCATGG 1801 TTACTCGAGG CCACCGACTC TACCAGCCGA AGTTGGCGTC CAACTATGTG TATGAGGTGA 1861 TGCTGAGATG TTGGCAGGAG AAACCAGAGG GAAGGCCTTC TTTCGAAGAT CTGCTGCGCA 1921 CAATAGATGA ACTAGTTGAA TGTGAAGAAA CTTTTGGAAG ACCAACTTTC TTGTACAAAG 1981 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 2041 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 2101 ACGACGGCTA AAATTGACTC AAGCCGTCAC GCGTTAAGTC gacaatcaac ctctggatta 2161 caaaatttgt gaaagatt