Construct: ORF TRCN0000473411

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018973.2_s317c1
Derived from:
ccsbBroadEn_14832
DNA Barcode:
AAGCCAAGCGATTACCGCTTACGG
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
RPS6KA3 (6197)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000473411
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 6197 RPS6KA3 ribosomal protein S6 kinase A3 NM_004586.3 99.5% 30.5% (many diffs)
2 human 6197 RPS6KA3 ribosomal protein S6 kinase A3 XM_005274573.2 99.4% 30.5% (many diffs)
3 human 6197 RPS6KA3 ribosomal protein S6 kinase A3 XM_011545556.1 98.6% 30.3% (many diffs)
4 human 6197 RPS6KA3 ribosomal protein S6 kinase A3 XM_011545555.1 97.6% 30.2% (many diffs)
5 human 6197 RPS6KA3 ribosomal protein S6 kinase A3 XM_011545563.3 95.8% 26.7% (many diffs)
6 human 6197 RPS6KA3 ribosomal protein S6 kinase A3 XM_017029713.1 95.8% 26.7% (many diffs)
7 human 6197 RPS6KA3 ribosomal protein S6 kinase A3 XM_017029714.2 95.8% 26.7% (many diffs)
8 human 6197 RPS6KA3 ribosomal protein S6 kinase A3 XM_017029715.2 95.8% 26.7% (many diffs)
9 human 6197 RPS6KA3 ribosomal protein S6 kinase A3 XM_017029716.1 95.8% 26.7% (many diffs)
10 human 6197 RPS6KA3 ribosomal protein S6 kinase A3 XM_017029717.2 95.8% 26.7% (many diffs)
11 human 6197 RPS6KA3 ribosomal protein S6 kinase A3 XM_006724507.3 95.6% 26.7% (many diffs)
12 human 6197 RPS6KA3 ribosomal protein S6 kinase A3 XM_017029719.2 95.6% 26.7% (many diffs)
13 human 6197 RPS6KA3 ribosomal protein S6 kinase A3 XM_011545557.2 95% 26.5% (many diffs)
14 human 6197 RPS6KA3 ribosomal protein S6 kinase A3 XM_011545558.2 95% 26.5% (many diffs)
15 human 6197 RPS6KA3 ribosomal protein S6 kinase A3 XM_011545560.1 95% 26.5% (many diffs)
16 human 6197 RPS6KA3 ribosomal protein S6 kinase A3 XM_011545561.2 95% 26.5% (many diffs)
17 human 6197 RPS6KA3 ribosomal protein S6 kinase A3 XM_017029718.2 94.3% 24.8% (many diffs)
18 human 6197 RPS6KA3 ribosomal protein S6 kinase A3 XM_005274577.3 94.2% 24.9% (many diffs)
19 human 6197 RPS6KA3 ribosomal protein S6 kinase A3 XM_011545562.2 93.6% 24.6% (many diffs)
20 mouse 110651 Rps6ka3 ribosomal protein S6 kinase... NM_148945.2 94.1% 30.4% (many diffs)
21 mouse 110651 Rps6ka3 ribosomal protein S6 kinase... XM_017318353.1 91.2% 27.2% (many diffs)
22 mouse 110651 Rps6ka3 ribosomal protein S6 kinase... NM_001346675.1 90.4% 26.6% (many diffs)
23 mouse 110651 Rps6ka3 ribosomal protein S6 kinase... XM_006528687.1 90.4% 26.6% (many diffs)
24 mouse 110651 Rps6ka3 ribosomal protein S6 kinase... XM_017318354.1 90.4% 26.6% (many diffs)
25 mouse 110651 Rps6ka3 ribosomal protein S6 kinase... XM_017318355.1 90.4% 26.6% (many diffs)
26 mouse 110651 Rps6ka3 ribosomal protein S6 kinase... XM_006528688.2 89.1% 24.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
780
ORF length:
714
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaagg
61ttggcatgcc gctggcgcag ctggcggacc cgtggcagaa gatggctgtg gagagcccgt
121ccgacagcgc tgagaatgga cagcaaatta tggatgaacc tatgggagag gaggagatta
181acccacaaac tgaagaagtc agtatcaaag aaattgcaat cacacatcat gtaaaggaag
241gacatgaaaa ggcagatcct tcccagtttg aacttttaaa agtattaggg cagggatcat
301ttggaaaggt tttcttagtt aaaaaaatct caggctctga tgctaggcag ctttatgcca
361tgaaggtatt gaagaaggcc acactgaaag ttcgagaccg agttcggaca aaaatggaac
421gtgatatctt ggtagaggtt aatcatcctt ttattgtcaa gttgcattat gcttttcaaa
481ctgaagggaa gttgtatctt attttggatt ttctcagggg aggagatttg tttacacgct
541tatccaaaga ggtgatgttc acagaagaag atgtcaaatt ctacttggct gaacttgcac
601ttgctttaga ccatctacat agcctgggaa taatttatag agacttaaaa ccagaaaata
661tacttcttga tgaagaaagt cacatcaagt taacagattt cggcctaagt aaagagtcta
721ttgaccatga aaagaagcat attctttttg tggaactgtg gagtatatgg ctccagaagt
781agttaatcgt cgaggtcata ctcagagtgc tgactggtgg tcttttggtg tgttaatgtt
841tgaaatgctt actggtacac tccctttcca aggaaaagat cgaaaagaaa caatgactat
901gattcttaaa gccaaacttg gaatgccaca gtttttgagt cctgaagcgc agagtctttt
961acgaatgctt ttcaagcgaa atcctgcaaa cagattaggt gcaggaccag atggagttga
1021agaaattaaa agacattcat ttttctcaac gatagactgg aataaactgt atagaagaga
1081aattcatccg ccatttaaac ctgcaacggg caggcctgaa gatacattct attttgatcc
1141tgagtttact gcaaaaactc ccaaagattc acctggcatt ccacctagtg ctaatgcaca
1201tcagcttttt cgggggttta gttttgttgc tattacctca gatgatgaaa gccaagctat
1261gcagacagtt ggtgtacatt caattgttca gcagttacac aggaacagta ttcagtttac
1321tgatggatat gaagtaaaag aagatattgg agttggctcc tactctgttt gcaagagatg
1381tatacataaa gctacaaaca tggagtttgc agtgaagatt attgataaaa gcaagagaga
1441cccaacagaa gaaattgaaa ttcttcttcg ttatggacag catccaaaca tatcacctct
1501aaaggaatgt atatgatgat ggaaagtatg tgtatgtagt aacagaactt atgaaaggag
1561tgaatggctg gataaaattt tagacaaaat ttttctctga acgagaggcc agtgctgtcc
1621tgttcactat aactaaaacc gttgaatatc ttcacgcaca aggggtggtt catagagact
1681tgaaacctag caacattctt tatgtggatg aatctggtaa tccggaatct attcgaattt
1741gtgattttgg ctttgcaaaa cagctgagag cggaaaatgg tcttctcatg actccttgtt
1801acactgcaaa ttttgttgca ccagaggttt taaaaagaca aggctatgat gctgcttgtg
1861atatatggag tcttggtgtc ctactctata caatgcttac cggttacact ccatttgcaa
1921atggtcctga tgatacacca gaggaaatat tGGCACGAAT AGGTAGCGGA AAATTCTCAC
1981TCAGTGGTGG TTACTGGAAT TCTGTTTCAG ACACAGCAAA GGACCTGGTG TCAAAGATGC
2041TTCATGTAGA CCCTCATCAG AGACTGACTG CTGCTCTTGT GCTCAGACAT CCTTGGATCG
2101TCCACTGGGA CCAACTGCCA CAATACCAAC TAAACAGACA GGATGCACCA CATCTAGTAA
2161AGGGTGCCAT GGCAGCTACA TATTCTGCTT TGAACCGTAA TCAGTCACCA GTTTTGGAAC
2221CAGTAGGCCG CTCTACTCTT GCTCAGCGGA GAGGTATTAA AAAAATCACC TCAACAGCCC
2281TGTTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC
2341TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT
2401GGCTTTATAT ATCTTGTGGA AAGGACGAAA GCCAAGCGAT TACCGCTTAC GGACGCGTTA
2461AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt

Download FASTA (ORF) (Full)