Construct: ORF TRCN0000473415
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006616.1_s317c1
- Derived from:
- ccsbBroadEn_05283
- DNA Barcode:
- TGCGATAGCCCATGGCGGTGGTCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC17A8 (246213)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473415
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 246213 | SLC17A8 | solute carrier family 17 me... | NM_001145288.2 | 100% | 100% | |
| 2 | human | 246213 | SLC17A8 | solute carrier family 17 me... | NM_139319.3 | 91.5% | 91.5% | 904_1053del |
| 3 | mouse | 216227 | Slc17a8 | solute carrier family 17 (s... | NM_182959.3 | 78.8% | 82.5% | (many diffs) |
| 4 | mouse | 216227 | Slc17a8 | solute carrier family 17 (s... | NM_001310710.1 | 54.4% | 57.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1686
- ORF length:
- 1617
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gccttttaaa gcatttgata ccttcaaaga aaaaattctg aaacctggga 121 aggaaggagt gaagaacgcc gtgggagatt ctttgggaat tttacaaaga aaaatcgatg 181 ggacaactga ggaagaagat aacattgagc tgaatgaaga aggaaggccg gtgcagacgt 241 ccaggccaag ccccccactc tgcgactgcc actgctgcgg cctccccaag cgttacatca 301 ttgctatcat gagtgggctg ggattctgca tttcctttgg gatccggtgc aatcttggag 361 ttgccattgt ggaaatggtc aacaatagca ccgtatatgt tgatggaaaa ccggaaattc 421 agacagcaca gtttaactgg gatccagaaa cagtgggcct tatccatgga tcttttttct 481 ggggctatat tatgacacaa attccaggtg gtttcatttc aaacaagttt gctgctaaca 541 gggtctttgg agctgccatc ttcttaacat cgactctgaa catgtttatt ccctctgcag 601 ccagagtgca ttacggatgc gtcatgtgtg tcagaattct gcaaggttta gtggagggtg 661 tgacctaccc agcctgccat gggatgtgga gtaagtgggc accacctttg gagagaagcc 721 gactggccac aacctctttt tgtggttcct atgcaggggc agtggttgcc atgcccctgg 781 ctggggtgtt ggtgcagtac attggatggt cctctgtctt ttatatttat ggcatgtttg 841 ggattatttg gtacatgttt tggctgttgc aggcctatga gtgcccagca gctcatccaa 901 caatatccaa tgaggagaag acctatatag agacaagcat aggagagggg gccaacgtgg 961 ttagtctaag tgtgggtctc ttgtcagcag tcccacacat ggttatgaca atcgttgtac 1021 ctattggagg acaattggct gattatttaa gaagcagaca aattttaacc acaactgctg 1081 tcagaaaaat catgaactgt ggaggttttg gcatggaggc aaccttactc ctggtggttg 1141 gcttttcgca taccaaaggg gtggctatct cctttctggt acttgctgta ggatttagtg 1201 gcttcgctat ttcaggtttt aatgtcaacc acctggacat tgccccacgc tatgccagca 1261 ttctcatggg gatctcaaac ggagtgggaa ccctctctgg aatggtctgt cccctcattg 1321 tcggtgcaat gaccaggcac aagacccgtg aagaatggca gaatgtgttc ctcatagctg 1381 cccTGGTGCA TTACAGTGGT GTGATCTTCT ATGGGGTCTT TGCTTCTGGG GAGAAACAGG 1441 AGTGGGCTGA CCCAGAGAAT CTCTCTGAGG AGAAATGTGG AATCATTGAC CAGGACGAAT 1501 TAGCTGAGGA GATAGAACTC AACCATGAGA GTTTTGCGAG TCCCAAAAAG AAGATGTCTT 1561 ATGGAGCCAC CTCCCAGAAT TGTGAAGTCC AGAAGAAGGA ATGGAAAGGA CAGAGAGGAG 1621 CGACCCTTGA TGAGGAAGAG CTGACATCCT ACCAGAATGA AGAGAGAAAC TTCTCAACTA 1681 TATCCTTGCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1741 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAGTA TTTCGATTTC 1801 TTGGCTTTAT ATATCTTGTG GAAAGGACGA TGCGATAGCC CATGGCGGTG GTCGACGCGT 1861 TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt