Construct: ORF TRCN0000473416
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014370.1_s317c1
- Derived from:
- ccsbBroadEn_06973
- DNA Barcode:
- AATTTCCCGCCATCCAGTCTCCGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC22A1 (6580)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473416
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6580 | SLC22A1 | solute carrier family 22 me... | NM_003057.3 | 99.9% | 99.8% | 480G>C |
| 2 | human | 6580 | SLC22A1 | solute carrier family 22 me... | XM_005267103.2 | 93.1% | 84.6% | 480G>C;1498_1585del;1719_1720ins31 |
| 3 | human | 6580 | SLC22A1 | solute carrier family 22 me... | NM_153187.1 | 91.2% | 83.2% | 480G>C;1384_1385ins113;1518_1519ins31 |
| 4 | human | 6580 | SLC22A1 | solute carrier family 22 me... | XM_006715552.2 | 87.1% | 87% | 480G>C;1383_1384ins213 |
| 5 | human | 6580 | SLC22A1 | solute carrier family 22 me... | XM_005267102.5 | 80.8% | 78.2% | 480G>C;1497_1498ins100;1563_1830del |
| 6 | human | 6580 | SLC22A1 | solute carrier family 22 me... | XM_005267104.5 | 51% | 48.5% | 0_1ins576;921_922ins100;987_1254del |
| 7 | human | 6580 | SLC22A1 | solute carrier family 22 me... | XM_005267105.5 | 51% | 48.5% | 0_1ins576;921_922ins100;987_1254del |
| 8 | human | 6580 | SLC22A1 | solute carrier family 22 me... | XM_011536074.3 | 51% | 48.5% | 0_1ins576;921_922ins100;987_1254del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1731
- ORF length:
- 1662
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcccaccgtg gatgacattc tggagcaggt tggggagtct ggctggttcc 121 agaagcaagc cttcctcatc ttatgcctgc tgtcggctgc ctttgcgccc atctgtgtgg 181 gcatcgtctt cctgggtttc acacctgacc accactgcca gagtcctggg gtggctgagc 241 tgagccagcg ctgtggctgg agccctgcgg aggagctgaa ctatacagtg ccaggcctgg 301 ggcccgcggg cgaggccttc cttggccagt gcaggcgcta tgaagtggac tggaaccaga 361 gcgccctcag ctgtgtagac cccctggcta gcctggccac caacaggagc cacctgccgc 421 tgggtccctg ccaggatggc tgggtgtatg acacgcccgg ctcttccatc gtcactgagt 481 tcaacctggt gtgtgctgac tcctggaagc tggacctctt tcagtcctgt ttgaatgcgg 541 gcttcttctt tggctctctc ggtgttggct actttgcaga caggtttggc cgtaagctgt 601 gtctcctggg aactgtgctg gtcaacgcgg tgtcgggcgt gctcatggcc ttctcgccca 661 actacatgtc catgctgctc ttccgcctgc tgcagggcct ggtcagcaag ggcaactgga 721 tggctggcta caccctaatc acagaatttg ttggctcggg ctccagaaga acggtggcga 781 tcatgtacca gatggccttc acggtggggc tggtggcgct taccgggctg gcctacgccc 841 tgcctcactg gcgctggctg cagctggcag tctccctgcc caccttcctc ttcctgctct 901 actactggtg tgtgccggag tcccctcggt ggctgttatc acaaaaaaga aacactgaag 961 caataaagat aatggaccac atcgctcaaa agaatgggaa gttgcctcct gctgatttaa 1021 agatgctttc cctcgaagag gatgtcaccg aaaagctgag cccttcattt gcagacctgt 1081 tccgcacgcc gcgcctgagg aagcgcacct tcatcctgat gtacctgtgg ttcacggact 1141 ctgtgctcta tcaggggctc atcctgcaca tgggcgccac cagcgggaac ctctacctgg 1201 atttccttta ctccgctctg gtcgaaatcc cgggggcctt catagccctc atcaccattg 1261 accgcgtggg ccgcatctac cccatggcca tgtcaaattt gttggcgggg gcagcctgcc 1321 tcgtcatgat ttttatctca cctgacctgc actggttaaa catcataatc atgtgtgttg 1381 gccgaatggg aatcaccatt gcaatacaaa tgatctgcct ggtgaatgcT GAGCTGTACC 1441 CCACATTCGT CAGGAACCTC GGAGTGATGG TGTGTTCCTC CCTGTGTGAC ATAGGTGGGA 1501 TAATCACCCC CTTCATAGTC TTCAGGCTGA GGGAGGTCTG GCAAGCCTTG CCCCTCATTT 1561 TGTTTGCGGT GTTGGGCCTG CTTGCCGCGG GAGTGACGCT ACTTCTTCCA GAGACCAAGG 1621 GGGTCGCTTT GCCAGAGACC ATGAAGGACG CCGAGAACCT TGGGAGAAAA GCAAAGCCCA 1681 AAGAAAACAC GATTTACCTT AAGGTCCAAA CCTCAGAACC CTCGGGCACC TTGCCAACTT 1741 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1801 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1861 CTTGTGGAAA GGACGAAATT TCCCGCCATC CAGTCTCCGG ACGCGTTAAG TCgacaatca 1921 acctctggat tacaaaattt gtgaaagatt