Transcript: Human NM_003057.3

Homo sapiens solute carrier family 22 member 1 (SLC22A1), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-09-27
Taxon:
Homo sapiens (human)
Gene:
SLC22A1 (6580)
Length:
1922
CDS:
122..1786

Additional Resources:

NCBI RefSeq record:
NM_003057.3
NBCI Gene record:
SLC22A1 (6580)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_003057.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000043210 CGATTTACCTTAAGGTCCAAA pLKO.1 1743 CDS 100% 4.950 6.930 N SLC22A1 n/a
2 TRCN0000043212 CCTGCACTGGTTAAACATCAT pLKO.1 1399 CDS 100% 4.950 3.465 N SLC22A1 n/a
3 TRCN0000043209 GCTACACCCTAATCACAGAAT pLKO.1 780 CDS 100% 4.950 3.465 N SLC22A1 n/a
4 TRCN0000043208 CCTCTTTCAGTCCTGTTTGAA pLKO.1 568 CDS 100% 5.625 3.375 N SLC22A1 n/a
5 TRCN0000043211 CCTGCTGATTTAAAGATGCTT pLKO.1 1061 CDS 100% 3.000 1.800 N SLC22A1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_003057.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06973 pDONR223 100% 99.9% 99.8% None 480G>C n/a
2 ccsbBroad304_06973 pLX_304 0% 99.9% 99.8% V5 480G>C n/a
3 TRCN0000473416 AATTTCCCGCCATCCAGTCTCCGG pLX_317 18.5% 99.9% 99.8% V5 480G>C n/a
4 TRCN0000488994 GTGGTTCAGACGCCCATGGGTATT pLX_317 21.6% 99.8% 99.6% V5 (not translated due to prior stop codon) 480G>C;1222A>G n/a
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