Construct: ORF TRCN0000473448

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000988.1_s317c1
Derived from:
ccsbBroadEn_00314
DNA Barcode:
TAGCCCCGACCAGACGGTTAACAC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CKMT1B (1159)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000473448
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1159 CKMT1B creatine kinase, mitochondr... NM_020990.4 100% 100%
2 human 1159 CKMT1B creatine kinase, mitochondr... XM_011521197.2 100% 100%
3 human 548596 CKMT1A creatine kinase, mitochondr... NM_001015001.2 99.8% 100% 1056G>A;1086C>T
4 human 548596 CKMT1A creatine kinase, mitochondr... NM_001321926.1 99.8% 100% 1056G>A;1086C>T
5 human 1159 CKMT1B creatine kinase, mitochondr... XM_011521194.1 93% 93% 148_240del
6 human 1159 CKMT1B creatine kinase, mitochondr... XM_011521195.2 93% 93% 148_240del
7 human 1159 CKMT1B creatine kinase, mitochondr... XM_011521196.1 93% 93% 148_240del
8 human 548596 CKMT1A creatine kinase, mitochondr... NM_001321927.1 92.9% 93% 148_240del;1149G>A;1179C>T
9 human 548596 CKMT1A creatine kinase, mitochondr... NM_001321928.1 92.9% 93% 148_240del;1149G>A;1179C>T
10 human 548596 CKMT1A creatine kinase, mitochondr... XM_017022369.1 92.9% 93% 148_240del;1149G>A;1179C>T
11 human 548596 CKMT1A creatine kinase, mitochondr... XM_017022370.1 92.9% 93% 148_240del;1149G>A;1179C>T
12 human 1159 CKMT1B creatine kinase, mitochondr... XM_011521198.1 88.9% 88.7% (many diffs)
13 human 1159 CKMT1B creatine kinase, mitochondr... XM_005254150.4 61.8% 60.9% 0_1ins433;11_12ins44
14 human 1159 CKMT1B creatine kinase, mitochondr... XM_011521199.2 61.8% 60.9% 0_1ins433;11_12ins44
15 human 1159 CKMT1B creatine kinase, mitochondr... XM_017021902.1 61.8% 60.9% 0_1ins433;11_12ins44
16 human 548596 CKMT1A creatine kinase, mitochondr... NM_001321929.1 61.7% 60.9% (many diffs)
17 human 548596 CKMT1A creatine kinase, mitochondr... XM_005254498.4 61.7% 60.9% (many diffs)
18 human 548596 CKMT1A creatine kinase, mitochondr... NR_135856.1 59.4% (many diffs)
19 human 1159 CKMT1B creatine kinase, mitochondr... NR_135751.1 46.3% (many diffs)
20 human 1159 CKMT1B creatine kinase, mitochondr... NR_135750.1 44.5% 1_589del;1464_2280del;2658_2809del
21 human 1159 CKMT1B creatine kinase, mitochondr... NR_135748.1 43.7% (many diffs)
22 human 1159 CKMT1B creatine kinase, mitochondr... NR_135749.1 41.8% (many diffs)
23 human 548596 CKMT1A creatine kinase, mitochondr... XM_017022371.1 41.2% 34.5% 148_240del;537_538ins44;648_649ins652
24 mouse 12716 Ckmt1 creatine kinase, mitochondr... NM_001355069.1 92.1% 96.6% (many diffs)
25 mouse 12716 Ckmt1 creatine kinase, mitochondr... NM_009897.3 92.1% 96.6% (many diffs)
26 mouse 12716 Ckmt1 creatine kinase, mitochondr... XM_006498656.3 92.1% 96.6% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1320
ORF length:
1251
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggctggtccc ttctcccgtc tgctgtccgc ccgcccggga ctcaggctcc
121tggctttggc cggagcgggg tctctagccg ctgggtttct gctccgaccg gaacctgtac
181gagctgccag tgaacgacgg aggctgtatc ccccgagcgc tgagtaccca gacctccgaa
241agcacaacaa ctgcatggcc agtcacctga ccccagcagt ctatgcacgg ctctgcgaca
301agaccacacc cactggttgg acgctagatc agtgtatcca gactggcgtg gacaaccctg
361gccacccctt catcaagact gtgggcatgg tggctggaga tgaggagacc tatgaggtat
421ttgctgacct gtttgaccct gtgatccaag agcgacacaa tggatatgac ccccggacaa
481tgaagcacac cacggatcta gatgccagta aaatccgttc tggctacttt gatgagaggt
541atgtattgtc ctctagagtc agaactggcc gaagcatccg aggactcagt ctgcctccag
601cttgcactcg agcagagcga cgagaggtgg aacgtgttgt ggtggatgca ctgagtggcc
661tgaagggtga cctggctgga cgttactata ggctcagtga gatgacagag gctgaacagc
721agcagcttat tgatgaccac tttctgtttg ataagcctgt gtccccgttg ctgactgcag
781caggaatggc tcgagactgg ccagatgctc gtggaatttg gcacaacaat gagaagagct
841tccTGATCTG GGTGAATGAG GAGGATCATA CACGGGTGAT CTCCATGGAG AAGGGTGGTA
901ACATGAAGAG AGTGTTTGAA AGATTCTGCC GAGGCCTCAA AGAGGTGGAG AGACTTATCC
961AAGAACGTGG CTGGGAGTTC ATGTGGAATG AGCGTTTGGG ATACATCTTG ACCTGTCCAT
1021CTAACCTGGG CACTGGACTT CGGGCAGGAG TGCACATCAA ACTGCCCCTG CTAAGCAAAG
1081ATAGCCGCTT CCCAAAGATC CTGGAGAACC TAAGACTCCA AAAACGTGGT ACTGGAGGAG
1141TGGACACTGC TGCTACAGGC GGTGTCTTTG ATATTTCTAA TTTGGACCGA CTAGGCAAAT
1201CAGAGGTGGA GCTGGTGCAA CTGGTCATCG ATGGAGTAAA CTATTTGATT GATTGTGAAC
1261GGCGTCTGGA GAGAGGCCAG GATATCCGCA TCCCCACACC TGTCATCCAC ACCAAGCATT
1321TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG
1381GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC
1441TTTATATATC TTGTGGAAAG GACGATAGCC CCGACCAGAC GGTTAACACA CGCGTTAAGT
1501Cgacaatcaa cctctggatt acaaaatttg tgaaagatt

Download FASTA (ORF) (Full)