Construct: ORF TRCN0000473480
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015675.1_s317c1
- Derived from:
- ccsbBroadEn_14184
- DNA Barcode:
- TAACTAACCTCAAGATTCCCCTTC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- PHIP (55023)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473480
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55023 | PHIP | pleckstrin homology domain ... | XM_017010989.2 | 66.6% | 66.2% | (many diffs) |
| 2 | human | 55023 | PHIP | pleckstrin homology domain ... | XM_017010990.2 | 66.6% | 66.2% | (many diffs) |
| 3 | human | 55023 | PHIP | pleckstrin homology domain ... | XM_011535918.3 | 51.1% | 50.8% | (many diffs) |
| 4 | human | 55023 | PHIP | pleckstrin homology domain ... | XM_005248729.5 | 46.3% | 46% | (many diffs) |
| 5 | human | 55023 | PHIP | pleckstrin homology domain ... | NM_017934.7 | 46.3% | 46% | (many diffs) |
| 6 | mouse | 83946 | Phip | pleckstrin homology domain ... | XM_011242840.2 | 59.3% | 61.3% | (many diffs) |
| 7 | mouse | 83946 | Phip | pleckstrin homology domain ... | XM_006511565.3 | 45.5% | 47% | (many diffs) |
| 8 | mouse | 83946 | Phip | pleckstrin homology domain ... | XM_006511564.3 | 41.2% | 42.6% | (many diffs) |
| 9 | mouse | 83946 | Phip | pleckstrin homology domain ... | NM_001081216.1 | 41.2% | 42.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2586
- ORF length:
- 2520
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ccggaaaaat aaaatatata gtatcaatcc caaaaaacaa ccatggcata 121 aaatggagct acgggaacaa gaacttatga aaatagttgg cataaagtat gaagtgggat 181 tacctaccct ttgctgcctt aaacttgctt ttctagatcc tgatactggt aaactgactg 241 gcggatcatt taccatgaaa taccatgata tgcctgacgt tatagatttc ctagtcttga 301 gacaacaatt tgatgatgca aaatacaggc gatggaatat aggtgaccgc ttcaggtctg 361 tcatagatga tgcctggtgg tttggaacaa tcgaaagcca ggaacctctt caacctgagt 421 accctgatag tctgtttcaa tgctacaatg tttgctggga caatggagat acagaaaaga 481 tgagtccttg ggatatggag cttataccta ataatgctgt atttcctgaa gaactaggta 541 ccagtgttcc tttaactgat ggtgagtgca gatcactaat ctataaacct cttgatggag 601 aatggggtac caatcccagg gatgaagaat gtgaaagaat tgtggcagga ataaaccagt 661 tgatgacact agatattgcc tcagcatttg tggcccccgt ggatctgcaa gcctatccca 721 tgtattgcac agtagtggca tatccaacgg atctaagtac aattaaacaa agactggaaa 781 acaggtttta caggcgggtt tcttccctaa tgtgggaagt tcgatatata gagcataata 841 cacgaacatt taatgagcct ggaagcccta ttgtgaaatc tgctaaattc gtgactgatc 901 ttcttctaca ttttataaag gatcagactt gttataacat aattccactt tataattcaa 961 tgaagaagaa agttttgtct gattctgagg atgaagagaa ggatgctgat gtgccaggaa 1021 cttctactcg aaaaaggaag gaccatcagc ctagaagaag attacgtaat agagcccagt 1081 cttacgatat tcaagcatgg aagaaacagt gtgaagaatt gttaaatctc atatttcaat 1141 gtgaagattc agagcctttc cgtcagccgg tagatctcct tgaatatcca gactacagag 1201 acatcattga cactccaatg gattttgcta ccgttagaga aactttagag gctgggaatt 1261 atgagtcacc aatggagtta tgtaaagatg tcagacttat tttcagtaat tccaaagcat 1321 atacaccaag caaaagatca aggatttaca gcatgagttt gcgcttgtct gcattctttg 1381 aagaacacat tagttcagtt ttatcagatt ataaatctgc tcttcgtttt cataaaagaa 1441 ataccataac caaaaggagg aagaaaagaa acagaagcag ctctgtttcc agtagtgctg 1501 catcaagccc tgaaaggaaa aaaaggatct taaaacccca gctaaaatca gaaagctcta 1561 cctctgcatt ctctacacct acacgatcaa taccgccaag acacaatgct gctcagataa 1621 acggtaaaac agaatctagt tctgtggttc gaaccagaag caaccgagtg gttgtagatc 1681 cagttgtcac tgagcaacca tctacttctt cagctgcaaa gacttttatt acaaaagcta 1741 atgcatctgc aataccaggg aaaacaatac tagagaattc tgtgaaacat tccaaagctt 1801 tgaatactct ttccagtcct ggtcaatcca gttttagtca tggcactagg aataattctg 1861 caaaagaaaa catggaaaag gaaaagccag tcaaacgtaa aatgaagtca tctgtactcc 1921 caaaggcgtc cactctttca aagtcatcag ctgtcattga gcaaggagat tgtaagaaca 1981 acgctcttgt accaggaacc attcaagtaa atggccatgg aggacagcca tcaaaacttg 2041 tgaagagggg acctggaagg aaacctaaag tagaagttaa taccaatagt ggtgaaatta 2101 tacacaagaa aaggggtaga aagcccaaaa agctacagta tgcaaagcca gaagatttag 2161 agcaaaataa tgtgcatccc aTCAGAGATG AAGTACTTCC TTCTTCAACA TGCAATTTTC 2221 TTTCTGAAAC TAATAATGTA AAGGAAGATT TGTTACAGAA AAAGAATCGT GGAGGTAGGA 2281 AGCCCAAAAG GAAGATGAAG ACACAAAAAT TAGATGCAGA TCTCCTAGTC CCTGCAAGTG 2341 TCAAAGTGTT AAGGAGAAGT AACCGAAAAA AGATAGATGA TCCTATAGAT GAGGAAGAAG 2401 AGTTTGAAGA ACTCAAAGGC TCTGAACCCC ACATGAGAAC TAGAAATCAA GGTCGAAGGA 2461 CAGCTTTCTA TAATGAGGAT GACTCTGAAG AGGAGCAAAG GCAGCTGTTG TTCGAAGACA 2521 CCTCTTTAAC TTTTGGAACT TCTAGTAGAG GACGAGTCCG AAAGTTGACT GAAAAAGCAA 2581 AAGCTTAATT TAATTGGTTG GTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG 2641 CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT 2701 GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGATAA CTAACCTCAA 2761 GATTCCCCTT CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat 2821 t