Transcript: Human XM_017010990.2

PREDICTED: Homo sapiens pleckstrin homology domain interacting protein (PHIP), transcript variant X4, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
PHIP (55023)
Length:
5572
CDS:
1632..5432

Additional Resources:

NCBI RefSeq record:
XM_017010990.2
NBCI Gene record:
PHIP (55023)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017010990.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000322839 TCGAACTGGCAGACGGATATT pLKO_005 478 5UTR 100% 13.200 18.480 N PHIP n/a
2 TRCN0000322908 TCGATATATAGAGCATAATAC pLKO_005 3650 CDS 100% 13.200 18.480 N PHIP n/a
3 TRCN0000147009 CGGATATTTACTGGTTCTGAT pLKO.1 491 5UTR 100% 4.950 6.930 N PHIP n/a
4 TRCN0000130419 GTTAAGGAGAAGTAACCGAAA pLKO.1 5177 CDS 100% 4.050 3.240 N PHIP n/a
5 TRCN0000350702 AGCACGTATTTGGCAATTTAA pLKO_005 1066 5UTR 100% 15.000 10.500 N PHIP n/a
6 TRCN0000359510 ATGGAGCTTATACCTAATAAT pLKO_005 3324 CDS 100% 15.000 10.500 N PHIP n/a
7 TRCN0000359508 CATGATATGCCTGACGTTATA pLKO_005 3093 CDS 100% 13.200 9.240 N PHIP n/a
8 TRCN0000147136 CCAGGAACCATTCAAGTAAAT pLKO.1 4821 CDS 100% 13.200 9.240 N PHIP n/a
9 TRCN0000322838 GATCATATTATTCGGGTTTAT pLKO_005 926 5UTR 100% 13.200 9.240 N PHIP n/a
10 TRCN0000130643 CCATGATATGCCTGACGTTAT pLKO.1 3092 CDS 100% 10.800 7.560 N PHIP n/a
11 TRCN0000149161 GCAGTGTATCAGCACATGAAA pLKO.1 404 5UTR 100% 5.625 3.938 N PHIP n/a
12 TRCN0000127738 GCTGGAAGACAGTCTTTACTA pLKO.1 209 5UTR 100% 5.625 3.938 N PHIP n/a
13 TRCN0000149327 GACAGTCTTTACTACGCACAA pLKO.1 216 5UTR 100% 4.050 2.835 N PHIP n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017010990.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14184 pDONR223 100% 66.6% 66.2% None (many diffs) n/a
2 ccsbBroad304_14184 pLX_304 0% 66.6% 66.2% V5 (not translated due to prior stop codon) (many diffs) n/a
3 TRCN0000473480 TAACTAACCTCAAGATTCCCCTTC pLX_317 16.8% 66.6% 66.2% V5 (not translated due to prior stop codon) (many diffs) n/a
4 ccsbBroadEn_12139 pDONR223 100% 55.7% 55.8% None 1_1677del;2563C>T;2571T>A n/a
5 ccsbBroad304_12139 pLX_304 0% 55.7% 55.8% V5 1_1677del;2563C>T;2571T>A n/a
6 TRCN0000470046 AGTGATCTGCATAGGTAATGGCGC pLX_317 20.3% 55.7% 55.8% V5 1_1677del;2563C>T;2571T>A n/a
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