Construct: ORF TRCN0000473493
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010723.1_s317c1
- Derived from:
- ccsbBroadEn_15811
- DNA Barcode:
- GCCACTGTCGCGGTGAAATGTGAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- WRAP73 (49856)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473493
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 49856 | WRAP73 | WD repeat containing, antis... | NM_017818.4 | 92.8% | 89.9% | (many diffs) |
| 2 | human | 49856 | WRAP73 | WD repeat containing, antis... | XM_017001387.2 | 91.3% | 88.2% | (many diffs) |
| 3 | human | 49856 | WRAP73 | WD repeat containing, antis... | XM_005244754.2 | 83.1% | 80.3% | (many diffs) |
| 4 | human | 49856 | WRAP73 | WD repeat containing, antis... | XR_946661.3 | 72% | (many diffs) | |
| 5 | mouse | 59002 | Wrap73 | WD repeat containing, antis... | NM_021499.2 | 79.1% | 80.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1371
- ORF length:
- 1305
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa cttctccgag gtattcaagc tctccagctt actctgcaag ttctccccgg 121 acggcaagta cctggcttcc tgtgtccagt accggttagt ggtccgggat gtgaacaccc 181 ttcagatcct tcagctgtac acgtgcctag accagatcca gcacatcgag tggtcggcag 241 actcgctctt catcctgtgc gccatgtaca agcgagggct ggtgcaggtc tggtctttag 301 agcagcccga atggcactgc aaaatagacg agggctcagc cgggctggtg gcctcgtgct 361 ggagcccgga cgggcgccac attctcaaca ccacggaatt ccatctgcgg ataaccgtct 421 ggtccttgtg cacaaaatcc gtgtcttaca tcaaataccc gaaagcttgt ctgcagggaa 481 tcaccttcac cagggacggc cgctacatgg cgctggcaga acggcgcgac tgcaaagatt 541 acgtgagcat cttcgtctgc agtgattggc agctcctgcg gcattttgat acggacaccc 601 aggatctcac agggattgag tgggccccaa acggctgtgt gctggcagtg tgggacacct 661 gcttggagta caagattctg ctgtactcat tggatggccg gttgttgtcc acgtacagcg 721 cttacgagtg gtccctgggc atcaagtctg tggcctggag ccccagcagt cagttcctgg 781 cagttgggag ctatgatgga aaggtgcgca tccttaatca cgtgacttgg aaaatgatca 841 cggagtttgg gcatcctgca gccattaatg atcccaagat agtggtgtat aaggaggccg 901 agaagagccc acagctggga ctgggctgcc tctccttccc gccgccccgg gccggggccg 961 gccctctccc gagctcagag agtaaatatg agatcgcctc tgtcccagtc tccttacaga 1021 cactgaaacc tgttaccgac agagcaaacc cgaaaatcgg cataggaatg ctggcattta 1081 gtcctgacag ctacttcctg gcgacaagga acgacaacat tcccaatGCC GTCTGGGTCT 1141 GGGACATTCA GAAGCTGAGG CTGTTCGCGG TGCTCGAGCA GCTGTCCCCA GTGCGCGCAT 1201 TTCAGTGGGA CCCGCAGCAG CCGCGGCTGG CCATCTGCAC GGGAGGCAGC AGGCTCTACC 1261 TGTGGTCCCC AGCGGGCTGC ATGTCGGTGC AGGTGCCTGG GGAAGGTAAG CACATCCCCG 1321 AGGCCACGGA CGTGGCAGCC TTGCTGTGTG CAGTCTGGCA TCACACGGGC TGCCCAACTT 1381 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1441 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1501 CTTGTGGAAA GGACGAGCCA CTGTCGCGGT GAAATGTGAT ACGCGTTAAG TCgacaatca 1561 acctctggat tacaaaattt gtgaaagatt