Construct: ORF TRCN0000473503

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF007769.1_s317c1
Derived from:
ccsbBroadEn_14312
DNA Barcode:
AGCACACTTCGTAACCTGTACTCC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CAPS2 (84698)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000473503
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 84698 CAPS2 calcyphosine 2 XM_017020039.1 58.1% 58% 1_438del;454G>A
2 human 84698 CAPS2 calcyphosine 2 NM_001286548.3 53.3% 53.1% 1_534del;550G>A
3 human 84698 CAPS2 calcyphosine 2 NM_001355030.2 53.3% 53.1% 1_534del;550G>A
4 human 84698 CAPS2 calcyphosine 2 NM_001355031.2 53.3% 53.1% 1_534del;550G>A
5 human 84698 CAPS2 calcyphosine 2 NM_001355032.2 53.3% 53.1% 1_534del;550G>A
6 human 84698 CAPS2 calcyphosine 2 NM_001355033.2 53.3% 53.1% 1_534del;550G>A
7 human 84698 CAPS2 calcyphosine 2 XM_017020036.1 53.3% 53.1% 1_534del;550G>A
8 human 84698 CAPS2 calcyphosine 2 NM_001355026.2 42.6% 42.5% 1_819del;835G>A
9 human 84698 CAPS2 calcyphosine 2 XM_005269194.3 41.8% 41.7% 1_438del;454G>A;879_879delTins172
10 human 84698 CAPS2 calcyphosine 2 XM_005269192.1 40% 39.9% 1_912del;928G>A
11 human 84698 CAPS2 calcyphosine 2 XM_011538888.1 40% 39.8% 1_915del;931G>A
12 human 84698 CAPS2 calcyphosine 2 NM_001355025.2 38.5% 38.4% 1_972del;988G>A
13 human 84698 CAPS2 calcyphosine 2 XM_011538887.2 37.4% 37.3% 1_1020del;1036G>A
14 human 84698 CAPS2 calcyphosine 2 NM_001355024.2 36.1% 35.9% 1_1080del;1096G>A
15 human 84698 CAPS2 calcyphosine 2 XM_024449226.1 35.9% 35.8% 1_1086del;1102G>A
16 human 84698 CAPS2 calcyphosine 2 NM_001355023.2 35.7% 35.6% 1_1098del;1114G>A
17 human 84698 CAPS2 calcyphosine 2 XM_011538886.2 35.3% 35.2% 1_1116del;1132G>A
18 human 84698 CAPS2 calcyphosine 2 XM_006719649.2 34.9% 34.8% 1_1134del;1150G>A
19 human 84698 CAPS2 calcyphosine 2 XM_011538878.1 33.8% 33.7% 1_1194del;1210G>A
20 human 84698 CAPS2 calcyphosine 2 XM_011538889.3 33.8% 33.7% 1_1194del;1210G>A
21 human 84698 CAPS2 calcyphosine 2 XM_006719648.2 33.1% 33% 1_1230del;1246G>A
22 human 84698 CAPS2 calcyphosine 2 NM_001355027.2 27.7% 27.6% 1_972del;988G>A;1413_1413delTins172
23 human 84698 CAPS2 calcyphosine 2 NM_001286547.3 27.5% 27.4% 1_984del;1000G>A;1425_1425delTins172
24 human 84698 CAPS2 calcyphosine 2 NM_032606.5 23.8% 23.7% 1_1230del;1246G>A;1671_1671delTins172
25 human 84698 CAPS2 calcyphosine 2 XR_944789.1 17% 1_1427del;1443G>A;2040_3585del
26 human 84698 CAPS2 calcyphosine 2 NR_149156.2 14.5% 1_681del;697G>A;1294_4207del
27 human 84698 CAPS2 calcyphosine 2 NR_149155.2 14.4% 1_709del;725G>A;1322_4235del
28 human 84698 CAPS2 calcyphosine 2 NR_149161.2 14.2% 1_755del;771G>A;1368_4281del
29 human 84698 CAPS2 calcyphosine 2 NR_149159.2 13.2% 1_1085del;1101G>A;1698_4611del
30 human 84698 CAPS2 calcyphosine 2 NR_149158.2 13.2% (many diffs)
31 human 84698 CAPS2 calcyphosine 2 NR_149157.2 13.1% 1_1113del;1129G>A;1726_4639del
32 human 84698 CAPS2 calcyphosine 2 NR_149160.2 13% 1_1145del;1161G>A;1758_4671del
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
681
ORF length:
612
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggaacaggag gataatataa tcattcaaga aaccaatgat aggctggtct
121tcaaagcaat tcaagatgtg ctaaaagaaa aactacataa aagaggtgtt cgtattttga
181ctggattggg aaaatatttt caacagttGG ACAAGGAAGG AAATGGACTT TTAGATAAGG
241CAGATTTTAA GCAAGCTCTA AAAGTGTTTC ACTTAGAAGT GTCTGAAAAG GATTTTGAGT
301CTGCATGGCT AATTCTGAAT GACAATGGCA ATGGCAAGGT TGATTATGGA GAATTCAAAC
361GTGGTATTAT TGGTGAAATG AATGAATACA GGAAATCATA TGTTCGAAAG GCCTTTATGA
421AACTGGATTT CAACAAAAGT GGCAGTGTGC CTATTATAAA CATAAGAAAA TGTTACTGTG
481CAAAGAAGCA TTCTCAAGTA ATTTCAGGCC ATTCAACAGA GGAAGAAATC AAATCATCCT
541TTCTAGAAAC ATTAAAAGTT GCCTGCAGCA AGTCTGATGA AGTGTCATAT GGTGAATTTG
601AAGATTACTA TGAAGGTTTA AGTATAGGAA TAGTAGATGA TGAAGACTTT GTTAACATCT
661TACGTACTCC ATGGGGGATT TTGCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC
721CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG
781AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAAGCA CACTTCGTAA
841CCTGTACTCC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)