Construct: ORF TRCN0000473516
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011028.1_s317c1
- Derived from:
- ccsbBroadEn_03898
- DNA Barcode:
- CGTTTAATGGTTTGATCGATGTCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SMAP1 (60682)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473516
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 60682 | SMAP1 | small ArfGAP 1 | NM_021940.5 | 100% | 100% | |
| 2 | human | 60682 | SMAP1 | small ArfGAP 1 | NM_001044305.3 | 94.2% | 94.2% | 415_495del |
| 3 | human | 60682 | SMAP1 | small ArfGAP 1 | XM_011536047.3 | 93.4% | 86.1% | (many diffs) |
| 4 | human | 60682 | SMAP1 | small ArfGAP 1 | NM_001281439.2 | 92.9% | 90.3% | (many diffs) |
| 5 | human | 60682 | SMAP1 | small ArfGAP 1 | NM_001281440.1 | 88.4% | 85.4% | (many diffs) |
| 6 | human | 60682 | SMAP1 | small ArfGAP 1 | XM_011536045.3 | 88.1% | 81.4% | (many diffs) |
| 7 | human | 60682 | SMAP1 | small ArfGAP 1 | XM_011536046.3 | 88% | 86.2% | (many diffs) |
| 8 | human | 60682 | SMAP1 | small ArfGAP 1 | XM_011536048.2 | 76% | 76% | 0_1ins255;160_240del |
| 9 | human | 60682 | SMAP1 | small ArfGAP 1 | XM_017011170.1 | 76% | 76% | 0_1ins255;160_240del |
| 10 | human | 60682 | SMAP1 | small ArfGAP 1 | XM_005248760.5 | 44.1% | 41.2% | (many diffs) |
| 11 | mouse | 98366 | Smap1 | small ArfGAP 1 | NM_028534.3 | 90.3% | 92% | (many diffs) |
| 12 | mouse | 98366 | Smap1 | small ArfGAP 1 | XM_006496362.1 | 85.1% | 86.7% | (many diffs) |
| 13 | mouse | 98366 | Smap1 | small ArfGAP 1 | NM_001290683.1 | 71.7% | 73% | (many diffs) |
| 14 | mouse | 98366 | Smap1 | small ArfGAP 1 | NR_110972.1 | 57.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1386
- ORF length:
- 1320
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttagcatggc gacgcgctcc tgtcgggaga aggctcagaa gctgaacgag cagcaccagc 121 tcatcctatc caagcttctg agggaggagg acaacaagta ctgcgccgac tgcgaggcca 181 aaggtcctcg atgggcttcc tggaatattg gtgtgtttat ttgcatcaga tgtgctggaa 241 ttcatagaaa tcttggggtt catatatcca gggtcaaatc agtcaaccta gaccaatgga 301 cagcagaaca gatacagtgc atgcaagata tgggaaatac taaagcaaga ctactctatg 361 aagccaatct tccagagaac tttcgaagac cacagacaga tcaagcagtg gaatttttca 421 tcagagataa atatgaaaag aagaaatact acgataaaaa tgccatagct attacaaata 481 aagaaaagga aaaaaaaaag gaagagaaaa agagagaaaa ggagccagaa aagccggcaa 541 aaccacttac agctgaaaag ctgcagaaga aagatcagca actggagcct aaaaaaagta 601 ccagccctaa aaaagctgcg gagcccactg tggatctttt aggacttgat ggccctgctg 661 tggcaccagt gaccaacggg aacacaacgg tgccacccct gaacgatgat ctggacatct 721 ttggaccgat gatttctaat cccttacctg caactgtcat gcccccagct caggggacac 781 cctctgcacc agcagctgca accctgtcta cagtaacatc tggggatcta gatttattca 841 ctgagcaaac tacaaaatca gaagaagtgg caaagaaaca actttccaaa gactccaTCT 901 TATCTCTGTA TGGCACAGGA ACCATTCAAC AGCAAAGTAC TCCTGGTGTA TTTATGGGAC 961 CCACAAATAT ACCATTTACC TCACAAGCAC CAGCTGCATT TCAGGGCTTT CCATCGATGG 1021 GCGTGCCTGT GCCTGCAGCT CCTGGCCTTA TAGGAAATGT GATGGGACAG AGTCCAAGCA 1081 TGATGGTGGG CATGCCCATG CCCAATGGGT TTATGGGAAA TGCACAAACT GGTGTGATGC 1141 CACTTCCTCA GAACGTTGTT GGCCCCCAAG GAGGAATGGT GGGACAAATG GGTGCACCCC 1201 AGAGTAAGTT TGGCCTGCCG CAAGCTCAGC AGCCCCAGTG GAGCCTCTCA CAGATGAATC 1261 AGCAGATGGC TGGCATGAGT ATCAGTAGTG CAACCCCTAC TGCAGGTTTT GGCCAGCCCT 1321 CCAGCACAAC AGCAGGATGG TCTGGAAGCT CATCAGGTCA GACTCTCAGC ACACAACTGT 1381 GGAAATGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1441 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGCCCGTA ACTTGAAAGT ATTTCGATTT 1501 CTTGGCTTTA TATATCTTGT GGAAAGGACG ACGTTTAATG GTTTGATCGA TGTCCACGCG 1561 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt