Construct: ORF TRCN0000473518
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005235.1_s317c1
- Derived from:
- ccsbBroadEn_06993
- DNA Barcode:
- TCCTACTTATTAACTTCATAGTAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SRD5A1 (6715)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473518
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6715 | SRD5A1 | steroid 5 alpha-reductase 1 | NM_001047.4 | 99.8% | 100% | 90C>G |
| 2 | human | 6715 | SRD5A1 | steroid 5 alpha-reductase 1 | NM_001324322.2 | 75.8% | 48.4% | 1_26del;116C>G;318_319ins167 |
| 3 | human | 6715 | SRD5A1 | steroid 5 alpha-reductase 1 | NM_001324323.2 | 67.6% | 63.7% | (many diffs) |
| 4 | human | 6719 | SRD5A1P1 | steroid 5 alpha-reductase 1... | NR_028597.1 | 34.3% | (many diffs) | |
| 5 | human | 6715 | SRD5A1 | steroid 5 alpha-reductase 1 | NR_136739.2 | 10.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 843
- ORF length:
- 777
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc aacggcgacg ggggtggcgg aggagcgcct gctggccgcg ctcgcctacc 121 tgcagtgcgc cgtgggctgc gcggtcttcg cgcggaatcg tcagacgaac tcagtgtacg 181 gccgccacgc gctgcccagc cacaggctcc gagtgccggc gcgggccgcc tgggtggtgc 241 aggagctgcc ctcgctggcc ctgccgctct accagtacgc cagcgagtcc gccccgcgtc 301 tccgcagcgc gcccaactgc atcctcctgg ccatgttcct cgtccactac gggcatcggt 361 gcttaattta cccatttctg atgcgaggag gaaagcctat gccactgttg gcgtgtacaa 421 tgGCGATTAT GTTCTGTACC TGTAACGGCT ATTTGCAAAG CAGATACTTG AGCCATTGTG 481 CAGTGTATGC TGATGACTGG GTAACAGATC CCCGTTTTCT AATAGGTTTT GGCTTGTGGT 541 TAACGGGCAT GTTGATAAAC ATCCATTCAG ATCATATCCT AAGGAATCTC AGAAAACCAG 601 GAGATACTGG ATACAAAATA CCAAGGGGAG GCTTATTTGA ATACGTAACT GCAGCCAACT 661 ATTTTGGAGA AATCATGGAG TGGTGTGGCT ATGCCCTGGC CAGCTGGTCT GTCCAAGGCG 721 CGGCTTTTGC TTTCTTCACG TTTTGTTTTT TATCTGGTAG AGCAAAAGAG CATCATGAGT 781 GGTACCTCCG GAAATTTGAA GAGTATCCAA AGTTCAGAAA AATTATAATT CCATTTTTGT 841 TTTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 901 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 961 GGCTTTATAT ATCTTGTGGA AAGGACGATC CTACTTATTA ACTTCATAGT AAACGCGTTA 1021 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt