Construct: ORF TRCN0000473522
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001603.1_s317c1
- Derived from:
- ccsbBroadEn_13181
- DNA Barcode:
- ACCTGGATCCAGCGCTCGGTCCGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ADAT2 (134637)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473522
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 134637 | ADAT2 | adenosine deaminase tRNA sp... | NM_001286259.1 | 90% | 90% | 0_1ins48 |
| 2 | human | 134637 | ADAT2 | adenosine deaminase tRNA sp... | XM_011535444.2 | 90% | 90% | 0_1ins48 |
| 3 | human | 134637 | ADAT2 | adenosine deaminase tRNA sp... | XM_017010260.2 | 90% | 90% | 0_1ins48 |
| 4 | human | 134637 | ADAT2 | adenosine deaminase tRNA sp... | XM_017010262.2 | 90% | 90% | 0_1ins48 |
| 5 | human | 134637 | ADAT2 | adenosine deaminase tRNA sp... | XM_024446329.1 | 90% | 90% | 0_1ins48 |
| 6 | human | 134637 | ADAT2 | adenosine deaminase tRNA sp... | NM_182503.3 | 83.7% | 83.7% | 1_93del |
| 7 | human | 134637 | ADAT2 | adenosine deaminase tRNA sp... | XM_017010263.1 | 81.6% | 77.3% | 0_1ins85;22_25delATGT |
| 8 | human | 134637 | ADAT2 | adenosine deaminase tRNA sp... | XM_011535439.2 | 69.2% | 69.1% | 1_210del;212_213delCTinsTG |
| 9 | human | 134637 | ADAT2 | adenosine deaminase tRNA sp... | XM_011535443.1 | 25.9% | 16.5% | (many diffs) |
| 10 | mouse | 66757 | Adat2 | adenosine deaminase, tRNA-s... | NM_025748.4 | 71.4% | 80.1% | (many diffs) |
| 11 | mouse | 66757 | Adat2 | adenosine deaminase, tRNA-s... | XM_011243200.2 | 49.5% | 44.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 546
- ORF length:
- 480
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc caaagaagcc ctcGAAAATA CTGAAGTTCC TGTTGGCTGT CTTATGGTCT 121 ACAACAATGA AGTTGTAGGG AAGGGGAGAA ATGAAGTTAA CCAAACCAAA AATGCTACTC 181 GACATGCAGA AATGGTGGCC ATCGATCAGG TCCTCGATTG GTGTCGTCAA AGTGGCAAGA 241 GTCCCTCTGA AGTATTTGAA CACACTGTGT TGTATGTCAC TGTGGAGCCG TGCATTATGT 301 GTGCAGCTGC TCTCCGCCTG ATGAAAATCC CGCTGGTTGT ATATGGCTGT CAGAATGAAC 361 GATTTGGTGG TTGTGGCTCT GTTCTAAATA TTGCCTCTGC TGACCTACCA AACACTGGGA 421 GACCATTTCA GTGTATCCCT GGATATCGGG CTGAGGAAGC AGTGGAAATG TTAAAGACCT 481 TCTACAAACA AGAAAATCCA AATGCACCAA AATCGAAAGT TCGGAAAAAG GAATGTCAGA 541 AATCTTGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 601 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 661 CTTGGCTTTA TATATCTTGT GGAAAGGACG AACCTGGATC CAGCGCTCGG TCCGCACGCG 721 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt