Construct: ORF TRCN0000473528
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001428.1_s317c1
- Derived from:
- ccsbBroadEn_08790
- DNA Barcode:
- GTCTCTAGAACAGGACTCGGACGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GPBP1L1 (60313)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473528
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 60313 | GPBP1L1 | GC-rich promoter binding pr... | NM_021639.5 | 99.8% | 100% | 1248A>G;1326T>C |
| 2 | human | 60313 | GPBP1L1 | GC-rich promoter binding pr... | XM_024448806.1 | 99.8% | 100% | 1248A>G;1326T>C |
| 3 | human | 60313 | GPBP1L1 | GC-rich promoter binding pr... | XM_024448808.1 | 99.8% | 100% | 1248A>G;1326T>C |
| 4 | human | 60313 | GPBP1L1 | GC-rich promoter binding pr... | XM_024448809.1 | 99.8% | 100% | 1248A>G;1326T>C |
| 5 | human | 60313 | GPBP1L1 | GC-rich promoter binding pr... | XM_024448810.1 | 99.8% | 100% | 1248A>G;1326T>C |
| 6 | human | 60313 | GPBP1L1 | GC-rich promoter binding pr... | XM_017002019.2 | 88.6% | 88.8% | 885_886ins159;1089A>G;1167T>C |
| 7 | human | 60313 | GPBP1L1 | GC-rich promoter binding pr... | XR_002957285.1 | 39.1% | (many diffs) | |
| 8 | human | 60313 | GPBP1L1 | GC-rich promoter binding pr... | XR_001737358.2 | 38.7% | (many diffs) | |
| 9 | mouse | 77110 | Gpbp1l1 | GC-rich promoter binding pr... | NM_029868.2 | 87.2% | 88.4% | (many diffs) |
| 10 | mouse | 77110 | Gpbp1l1 | GC-rich promoter binding pr... | XM_006503485.3 | 87.1% | 88.4% | (many diffs) |
| 11 | mouse | 77110 | Gpbp1l1 | GC-rich promoter binding pr... | XM_017320452.1 | 87.1% | 88.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1488
- ORF length:
- 1422
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gcagcatgat tttgttcctg cttggctaaa tttctcaaca ccacagtcag 121 ctaagtcacc tactgccacc ttcgaaaaac acggagagca cctacccaga ggagaaggta 181 gatttggagt aagccgccgt cgacataatt cctctgatgg tttttttaac aatggtcccc 241 tacgaactgc aggagattct tggcaccagc cctccctgtt ccgccatgat tctgtggact 301 ctggtgtctc taagggagca tatgctggaa tcacagggaa cccatctggt tggcatagct 361 cttcccgagg tcatgatggc atgagccaac gtagtggagg tggcacaggg aaccatcgcc 421 attggaatgg cagcttccac tcccggaaag ggtgtgcttt tcaggaaaag ccacctatgg 481 agattaggga agaaaagaaa gaagacaagg tggaaaagtt gcagtttgaa gaggaggact 541 ttccttcctt gaatccagaa gctggcaaac agcatcagcc atgcagacct attgggacac 601 cttctggagt atgggaaaac ccgcctagtg ccaagcaacc ctccaagatg ctagttatca 661 aaaaagtttc caaagaggat cctgctgctg ccttctctgc tgcattcacc tcaccaggat 721 ctcaccatgc aaatgggaac aaattgtcat ccgtggttcc aagtgtctat aagaacctgg 781 ttcctaagcc tgtaccacct ccttccaagc ctaatgcatg gaaagctaac aggatggagc 841 acaagtcagg atccctttcc tctagccggg agtctgcttt taccagtcca atctctgtta 901 ccaaaccagt ggtactggct agtggtgcag ctcTGAGTTC TCCCAAAGAG AGTCCCTCCA 961 GCACCACCCC TCCAATTGAG ATCAGCTCCT CTCGTCTGAC CAAGTTGACC CGCCGAACCA 1021 CCGACAGGAA GAGTGAGTTC CTGAAAACTC TGAAGGATGA CCGGAATGGA GACTTCTCAG 1081 AGAATAGAGA CTGTGACAAG CTGGAAGATT TGGAGGACAA CAGCACACCT GAACCAAAGG 1141 AAAATGGGGA GGAAGGCTGT CATCAAAATG GTCTTGCCCT CCCTGTAGTG GAAGAAGGGG 1201 AGGTTCTCTC ACACTCTCTA GAAGCAGAGC ACAGGTTATT GAAAGCTATG GGTTGGCAGG 1261 AATATCCTGA AAATGATGAG AATTGCCTTC CCCTCACAGA GGATGAGCTC AAGGAGTTCC 1321 ACATGAAGAC AGAGCAGCTG AGAAGAAATG GCTTTGGAAA GAATGGCTTC TTGCAGAGCC 1381 GCAGTTCCAG CCTGTTCTCC CCTTGGAGAA GCACTTGCAA AGCAGAGTTT GAGGACTCAG 1441 ACACCGAAAC CAGTAGCAGT GAAACATCAG ATGACGATGC CTGGAAGTAC CCAACTTTCT 1501 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1561 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1621 GTGGAAAGGA CGAGTCTCTA GAACAGGACT CGGACGGACG CGTTAAGTCg acaatcaacc 1681 tctggattac aaaatttgtg aaagatt