Construct: ORF TRCN0000473536
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004889.1_s317c1
- Derived from:
- ccsbBroadEn_07636
- DNA Barcode:
- CGACTAAACCGTAATGTCCTCCCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SPTLC1 (10558)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473536
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10558 | SPTLC1 | serine palmitoyltransferase... | NM_178324.3 | 99.7% | 99.3% | 418G>C |
| 2 | human | 10558 | SPTLC1 | serine palmitoyltransferase... | XM_011518138.2 | 38.9% | 38.6% | 418G>C;429_1095delinsA |
| 3 | human | 10558 | SPTLC1 | serine palmitoyltransferase... | NM_006415.4 | 30% | 29.8% | 418G>C;429_1419delinsA |
| 4 | human | 10558 | SPTLC1 | serine palmitoyltransferase... | NM_001281303.2 | 27.7% | 27.4% | 418G>C;429_1539delinsA |
| 5 | mouse | 268656 | Sptlc1 | serine palmitoyltransferase... | XM_006516927.1 | 44.5% | 47.2% | (many diffs) |
| 6 | mouse | 268656 | Sptlc1 | serine palmitoyltransferase... | NM_009269.2 | 25.3% | 26.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 519
- ORF length:
- 429
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGGCGACCGC CACGGAGCAG TGGGTTCTGG 121 TGGAGATGGT ACAGGCGCTT TACGAGGCTC CTGCTTACCA TCTTATTTTG GAAGGGATTC 181 TGATCCTCTG GATAATCAGA CTTCTTTTCT CTAAGACTTA CAAATTACAA GAACGATCTG 241 ATCTTACAGT CAAGGAAAAA GAAGAACTGA TTGAAGAGTG GCAACCAGAA CCTCTTGTTC 301 CTCCTGTCCC AAAAGACCAT CCTGCTCTCA ACTACAACAT CGTTTCAGGC CCTCCAAGCC 361 ACAAAACTGT GGTGAATGGA AAAGAATGTA TAAACTTCGC CTCATTTAAT TTTCTTGGAT 421 TGTTGGATAA CCCTAGGGTT AAGGCAGCAG CTTTAGCATC TCTAAAGAAG TATGGCGTGG 481 GGACTTGTGG ACCCAGAGGA TTTTATCGCA CATTTGAATG CCCAACTTTC TTGTACAAAG 541 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 601 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 661 ACGACGACTA AACCGTAATG TCCTCCCGAC GCGTTAAGTC gacaatcaac ctctggatta 721 caaaatttgt gaaagatt