Construct: ORF TRCN0000473539

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017112.3_s317c1
Derived from:
ccsbBroadEn_14538
DNA Barcode:
CAATATTCCATCCCCTGATACTAT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
AKT1 (207)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000473539
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 207 AKT1 AKT serine/threonine kinase 1 NM_001014431.2 100% 100%
2 human 207 AKT1 AKT serine/threonine kinase 1 NM_001014432.1 100% 100%
3 human 207 AKT1 AKT serine/threonine kinase 1 NM_005163.2 100% 100%
4 human 207 AKT1 AKT serine/threonine kinase 1 XR_002957536.1 33% (many diffs)
5 mouse 11651 Akt1 thymoma viral proto-oncogene 1 NM_001331107.1 90.9% 98.3% (many diffs)
6 mouse 11651 Akt1 thymoma viral proto-oncogene 1 NM_009652.3 90.9% 98.3% (many diffs)
7 mouse 11651 Akt1 thymoma viral proto-oncogene 1 XM_006515415.1 90.9% 98.3% (many diffs)
8 mouse 11651 Akt1 thymoma viral proto-oncogene 1 NM_001165894.1 83.3% 90.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1506
ORF length:
1440
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgag cgacgtggct attgtgaagg agggttggct gcacaaacga ggggagtaca
121tcaagacctg gcggccacgc tacttcctcc tcaagaatga tggcaccttc attggctaca
181aggagcggcc gcaggatgtg gaccaacgtg aggctcccct caacaacttc tctgtggcgc
241agtgccagct gatgaagacg gagcggcccc ggcccaacac cttcatcatc cgctgcctgc
301agtggaccac tgtcatcgaa cgcaccttcc atgtggagac tcctgaggag cgggaggagt
361ggacaaccgc catccagact gtggctgacg gcctcaagaa gcaggaggag gaggagatgg
421acttccggtc gggctcaccc agtgacaact caggggctga agagatggag gtgtccctgg
481ccaagcccaa gcaccgcgtg accatgaacg agtttgagta cctgaagctg ctgggcaagg
541gcactttcgg caaggtgatc ctggtgaagg agaaggccac aggccgctac tacgccatga
601agatcctcaa gaaggaagtc atcgtggcca aggacgaggt ggcccacaca ctcaccgaga
661accgcgtcct gcagaactcc aggcacccct tcctcacagc cctgaagtac tctttccaga
721cccacgaccg cctctgcttt gtcatggagt acgccaacgg gggcgagctg ttcttccacc
781tgtcccggga gcgtgtgttc tccgaggacc gggcccgctt ctatggcgct gagattgtgt
841cagccctgga ctacctgcac tcggagaaga acgtggtgta ccgggacctc aagctggaga
901acctcatgct ggacaaggac gggcacatta agatcacaga cttcgggctg tgcaaggagg
961ggatcaagga cggtgccacc atgaagacct tttgcggcac acctgagtac ctggcccccg
1021aggtgctgga ggacaatgac tacggccgtg cagtggactg gtgggggctg ggcgtggtca
1081tgtacgagat gatgtgcggt cgcctgccct tctacaacca ggaccatgag aagctttttg
1141agctcatcct catggaggag atccgcttcc cgcgcacgct tggtcccgag gccaagtcct
1201tgctttcagg gctgctcaag aaggacccca agcagaggct tggcgggggc tccgaggacg
1261ccaaggagat catgcagcat cgcttcTTTG CCGGTATCGT GTGGCAGCAC GTGTACGAGA
1321AGAAGCTCAG CCCACCCTTC AAGCCCCAGG TCACGTCGGA GACTGACACC AGGTATTTTG
1381ATGAGGAGTT CACGGCCCAG ATGATCACCA TCACACCACC TGACCAAGAT GACAGCATGG
1441AGTGTGTGGA CAGCGAGCGC AGGCCCCACT TCCCCCAGTT CTCCTACTCG GCCAGCGGCA
1501CGGCCTGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC
1561TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT
1621CTTGGCTTTA TATATCTTGT GGAAAGGACG ACAATATTCC ATCCCCTGAT ACTATACGCG
1681TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt

Download FASTA (ORF) (Full)

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