Construct: ORF TRCN0000473561

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF007438.1_s317c1
Derived from:
ccsbBroadEn_12569
DNA Barcode:
CGAAATTGACGTATAGCATAATCT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CDC73 (79577)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000473561
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 79577 CDC73 cell division cycle 73 NM_024529.4 66.8% 66.8% 1_528del
2 human 79577 CDC73 cell division cycle 73 XM_006711537.4 30.8% 28.4% (many diffs)
3 mouse 214498 Cdc73 cell division cycle 73, Paf... NM_145991.2 61.7% 66.8% (many diffs)
4 mouse 214498 Cdc73 cell division cycle 73, Paf... XM_006529365.1 28% 26.8% (many diffs)
5 mouse 214498 Cdc73 cell division cycle 73, Paf... XM_006529366.3 25.9% 27.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1131
ORF length:
1065
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgtc agtggaaaaa attgctgcaa tcaaagccaa aattatggct aagaaaagat
121ctactatcaa gactgatcta gatgatgaca taactgccct taaacagagg agttttgtgg
181atgctgaggt agatgtgacc cgagatattg tcagcagaga gagagtatgg aggacacgaa
241caactatctt acaaagcaca ggaaagaatt tttccaagaa catttttgca attcttcaat
301ctgtaaaagc cagagaagaa gggcgtgcac ctgaacagcg acctgcccca aatgcagcac
361ctgtggatcc cactttgcgc accaaacagc ctatcccagc tgcctataac agatacgatc
421aggaaagatt caaaggaaaa gaagaaacgg aaggcttcaa aattgacact atgggaacct
481accatggtat gacactgaaa tctgtaacgg agggtgcatc tgcccggaag actcagactc
541ctgcagccca gccagtacca agaccagttt ctcaagcaag acctccccca aatcagaaga
601aaggatctcg aacacccatt atcataattc ctgcagctac cacctcttta ataaccatgc
661ttaatgcaaa agaccttcta caggacctga aatttgtccc atcagatgaa aagaagaaac
721aaggttgtca acgagaaaat gaaactctaa tacaaagaag aaaagaccag atgcaaccag
781ggggcactgc aattagtgtt acagtacctt atagagtagt agaccagccc cttaaactta
841tgcctcaaga ctgggaccgc gttgtagccg tttttgtgca gggtcctgca tggcagttca
901aaggttggcc atggcttttg ccTGATGGAT CACCAGTTGA TATATTTGCT AAAATTAAAG
961CCTTCCATCT GAAGTATGAT GAAGTTCGTC TGGATCCAAA TGTTCAGAAA TGGGATGTAA
1021CAGTATTAGA ACTCAGCTAT CACAAACGTC ATTTGGATAG ACCAGTGTTC TTACGGTTTT
1081GGGAAACATT GGACAGGTAC ATGGTAAAGC ATAAATCGCA CTTGAGATTC TGCCCAACTT
1141TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT
1201CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT
1261CTTGTGGAAA GGACGACGAA ATTGACGTAT AGCATAATCT ACGCGTTAAG TCgacaatca
1321acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)