Construct: ORF TRCN0000473561
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007438.1_s317c1
- Derived from:
- ccsbBroadEn_12569
- DNA Barcode:
- CGAAATTGACGTATAGCATAATCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CDC73 (79577)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473561
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79577 | CDC73 | cell division cycle 73 | NM_024529.4 | 66.8% | 66.8% | 1_528del |
| 2 | human | 79577 | CDC73 | cell division cycle 73 | XM_006711537.4 | 30.8% | 28.4% | (many diffs) |
| 3 | mouse | 214498 | Cdc73 | cell division cycle 73, Paf... | NM_145991.2 | 61.7% | 66.8% | (many diffs) |
| 4 | mouse | 214498 | Cdc73 | cell division cycle 73, Paf... | XM_006529365.1 | 28% | 26.8% | (many diffs) |
| 5 | mouse | 214498 | Cdc73 | cell division cycle 73, Paf... | XM_006529366.3 | 25.9% | 27.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1131
- ORF length:
- 1065
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc agtggaaaaa attgctgcaa tcaaagccaa aattatggct aagaaaagat 121 ctactatcaa gactgatcta gatgatgaca taactgccct taaacagagg agttttgtgg 181 atgctgaggt agatgtgacc cgagatattg tcagcagaga gagagtatgg aggacacgaa 241 caactatctt acaaagcaca ggaaagaatt tttccaagaa catttttgca attcttcaat 301 ctgtaaaagc cagagaagaa gggcgtgcac ctgaacagcg acctgcccca aatgcagcac 361 ctgtggatcc cactttgcgc accaaacagc ctatcccagc tgcctataac agatacgatc 421 aggaaagatt caaaggaaaa gaagaaacgg aaggcttcaa aattgacact atgggaacct 481 accatggtat gacactgaaa tctgtaacgg agggtgcatc tgcccggaag actcagactc 541 ctgcagccca gccagtacca agaccagttt ctcaagcaag acctccccca aatcagaaga 601 aaggatctcg aacacccatt atcataattc ctgcagctac cacctcttta ataaccatgc 661 ttaatgcaaa agaccttcta caggacctga aatttgtccc atcagatgaa aagaagaaac 721 aaggttgtca acgagaaaat gaaactctaa tacaaagaag aaaagaccag atgcaaccag 781 ggggcactgc aattagtgtt acagtacctt atagagtagt agaccagccc cttaaactta 841 tgcctcaaga ctgggaccgc gttgtagccg tttttgtgca gggtcctgca tggcagttca 901 aaggttggcc atggcttttg ccTGATGGAT CACCAGTTGA TATATTTGCT AAAATTAAAG 961 CCTTCCATCT GAAGTATGAT GAAGTTCGTC TGGATCCAAA TGTTCAGAAA TGGGATGTAA 1021 CAGTATTAGA ACTCAGCTAT CACAAACGTC ATTTGGATAG ACCAGTGTTC TTACGGTTTT 1081 GGGAAACATT GGACAGGTAC ATGGTAAAGC ATAAATCGCA CTTGAGATTC TGCCCAACTT 1141 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1201 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1261 CTTGTGGAAA GGACGACGAA ATTGACGTAT AGCATAATCT ACGCGTTAAG TCgacaatca 1321 acctctggat tacaaaattt gtgaaagatt