Construct: ORF TRCN0000473568
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006195.1_s317c1
- Derived from:
- ccsbBroadEn_15499
- DNA Barcode:
- CTTTACAAGAATATATTATAATTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CD99 (4267)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473568
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4267 | CD99 | CD99 molecule (Xg blood group) | NM_002414.5 | 99.8% | 100% | 87C>T |
| 2 | human | 4267 | CD99 | CD99 molecule (Xg blood group) | NM_001321368.2 | 99.2% | 99.4% | 87C>T;474_475insGCA |
| 3 | human | 4267 | CD99 | CD99 molecule (Xg blood group) | NM_001122898.3 | 91.1% | 91.3% | 87C>T;97_98ins48 |
| 4 | human | 4267 | CD99 | CD99 molecule (Xg blood group) | NM_001321369.2 | 90.6% | 90.8% | 87C>T;97_98ins48;426_427insGCA |
| 5 | human | 4267 | CD99 | CD99 molecule (Xg blood group) | NM_001321370.2 | 90.6% | 90.8% | 87C>T;309_310ins51 |
| 6 | human | 4267 | CD99 | CD99 molecule (Xg blood group) | NM_001321367.2 | 86.1% | 85.4% | 87C>T;475_476insCAGAACAAGGGGAGGTGGAC;480_480delCins56 |
| 7 | human | 4267 | CD99 | CD99 molecule (Xg blood group) | NR_135623.2 | 48.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 621
- ORF length:
- 555
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ccgcggggct gcgctggcgc tgctgctctt cggcctgctg ggtgttctgg 121 tcgccgcccc ggatggtggt ttcgatttat ctgatgccct tcctgacaat gaaaacaaga 181 aacccactgc aatcccCAAG AAACCCAGTG CTGGGGATGA CTTTGACTTA GGAGATGCTG 241 TTGTTGATGG AGAAAATGAC GACCCACGAC CACCGAACCC ACCCAAACCG ATGCCAAATC 301 CAAACCCCAA CCACCCTAGT TCCTCCGGTA GCTTTTCAGA TGCTGACCTT GCGGATGGCG 361 TTTCAGGTGG AGAAGGAAAA GGAGGCAGTG ATGGTGGAGG CAGCCACAGG AAAGAAGGGG 421 AAGAGGCCGA CGCCCCAGGC GTGATCCCCG GGATTGTGGG GGCTGTCGTG GTCGCCGTGG 481 CTGGAGCCAT CTCTAGCTTC ATTGCTTACC AGAAAAAGAA GCTATGCTTC AAAGAAAATG 541 CAGAACAAGG GGAGGTGGAC ATGGAGAGCC ACCGGAATGC CAACGCAGAG CCAGCTGTTC 601 AGCGTACTCT TTTAGAGAAA TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 661 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 721 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGACTTT ACAAGAATAT 781 ATTATAATTG ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt