Construct: ORF TRCN0000473574
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015838.1_s317c1
- Derived from:
- ccsbBroadEn_02276
- DNA Barcode:
- ACCCATGACGATAAGAGAGACGCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MVP (9961)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473574
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9961 | MVP | major vault protein | NM_005115.5 | 100% | 100% | |
| 2 | human | 9961 | MVP | major vault protein | NM_017458.3 | 100% | 100% | |
| 3 | human | 9961 | MVP | major vault protein | NM_001293204.1 | 93.2% | 93.1% | 2234_2235ins180 |
| 4 | human | 9961 | MVP | major vault protein | NM_001293205.1 | 92.6% | 92.6% | 1435_1436ins198 |
| 5 | mouse | 78388 | Mvp | major vault protein | XM_011241952.2 | 83.4% | 88.1% | (many diffs) |
| 6 | mouse | 78388 | Mvp | major vault protein | NM_080638.3 | 82.5% | 87.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2745
- ORF length:
- 2679
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc aactgaagag ttcatcatcc gcatcccccc ataccactat atccatgtgc 121 tggaccagaa cagcaacgtg tcccgtgtgg aggtcgggcc aaagacctac atccggcagg 181 acaatgagag ggtactgttt gcccccatgc gcatggtgac cgtcccccca cgtcactact 241 gcacagtggc caaccctgtg tctcgggatg cccagggctt ggtgctgttt gatgtcacag 301 ggcaagttcg gcttcgccac gctgacctcg agatccggct ggcccaggac cccttccccc 361 tgtacccagg ggaggtgctg gaaaaggaca tcacacccct gcaggtggtt ctgcccaaca 421 ctgccctcca tctaaaggcg ctgcttgatt ttgaggataa agatggagac aaggtggtgg 481 caggagatga gtggcttttc gagggacctg gcacgtacat cccccggaag gaagtggagg 541 tcgtggagat cattcaggcc accatcatca ggcagaacca ggctctgcgg ctcagggccc 601 gcaaggagtg ctgggaccgg gacggcaagg agagggtgac aggggaagaa tggctggtca 661 ccacagtagg ggcgtacctc ccagcggtgt ttgaggaggt tctggatttg gtggacgccg 721 tcatccttac ggaaaagaca gccctgcacc tccgggctcg gcggaacttc cgggacttca 781 ggggagtgtc ccgccgcact ggggaggagt ggctggtaac agtgcaggac acagaggccc 841 acgtgccaga tgtccacgag gaggtgctgg gggttgtgcc catcaccacc ctgggccccc 901 acaactactg cgtgattctc gaccctgtcg gaccggatgg caagaatcag ctggggcaga 961 agcgcgtggt caagggagag aagtcttttt tcctccagcc aggagagcag ctggaacaag 1021 gcatccagga tgtgtatgtg ctgtcggagc agcaggggct gctgctgagg gccctgcagc 1081 ccctggagga gggggaggat gaggagaagg tctcacacca ggctggggac cactggctca 1141 tccgcggacc cctggagtat gtgccatctg ccaaagtgga ggtggtggag gagcgccagg 1201 ccatccctct agacgagaac gagggcatct atgtgcagga tgtcaagacc ggaaaggtgc 1261 gcgctgtgat tggaagcacc tacatgctga cccaggacga agtcctgtgg gagaaagagc 1321 tgcctcccgg ggtggaggag ctgctgaaca aggggcagga ccctctggca gacaggggtg 1381 agaaggacac agctaagagc ctccagccct tggcgccccg gaacaagacc cgtgtggtca 1441 gctaccgcgt gccccacaac gctgcggtgc aggtgtacga ctaccgagag aagcgagccc 1501 gcgtggtctt cgggcctgag ctggtgtcgc tgggtcctga ggagcagttc acagtgttgt 1561 ccctctcagc tgggcggccc aagcgtcccc atgcccgccg tgcgctctgc ctgctgctgg 1621 ggcctgactt cttcacagac gtcatcacca tcgaaacggc ggatcatgcc aggctgcaac 1681 tgcagctggc ctacaactgg cactttgagg tgaatgaccg gaaggacccc caagagacgg 1741 ccaagctctt ttcagtgcca gactttgtag gtgatgcctg caaagccatc gcatcccggg 1801 tgcggggggc cgtggcctct gtcactttcg atgacttcca taagaactca gcccgcatca 1861 ttcgcactgc tgtctttggc tttgagacct cggaagcgaa gggccccgat ggcatggccc 1921 tgcccaggcc ccgggaccag gctgtcttcc cccaaaacgg gctggtggtc agcagtgtgg 1981 acgtgcagtc agtggagcct gtggatcaga ggacccggga cgccctgcaa cgcagcgtcc 2041 agctggccat cgagatcacc accaactccc aggaagcggc ggccaagcat gaggctcaga 2101 gactggagca ggaagcccgc ggccggcttg agcggcagaa gatcctggac cagtcagaag 2161 ccgagaaagc tcgcaaggaa cttttggagc tggaggctct gagcatggcc gtggagagca 2221 ccgggactgc caaggcggag gccgagtccc gtgcggAGGC AGCCCGGATT GAGGGAGAAG 2281 GGTCCGTGCT GCAGGCCAAG CTAAAAGCAC AGGCCTTGGC CATTGAAACG GAGGCTGAGC 2341 TCCAGAGGGT CCAGAAGGTC CGAGAGCTGG AACTGGTCTA TGCCCGGGCC CAGCTGGAGC 2401 TGGAGGTGAG CAAGGCTCAG CAGCTGGCTG AGGTGGAGGT GAAGAAGTTC AAGCAGATGA 2461 CAGAGGCCAT AGGCCCCAGC ACCATCAGGG ACCTTGCTGT GGCTGGGCCT GAGATGCAGG 2521 TAAAACTGCT CCAGTCCCTG GGCCTGAAAT CAACCCTCAT CACCGATGGC TCCACTCCCA 2581 TCAACCTCTT CAACACAGCC TTTGGGCTGC TGGGGATGGG GCCCGAGGGT CAGCCCCTGG 2641 GCAGAAGGGT GGCCAGTGGG CCCAGCCCTG GGGAGGGGAT ATCCCCCCAG TCTGCTCAGG 2701 CCCCTCAAGC TCCTGGAGAC AACCACGTGG TGCCTGTACT GCGCTACCCA ACTTTCTTGT 2761 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 2821 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 2881 GAAAGGACGA ACCCATGACG ATAAGAGAGA CGCGACGCGT TAAGTCgaca atcaacctct 2941 ggattacaaa atttgtgaaa gatt