Construct: ORF TRCN0000473578
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001105.1_s317c1
- Derived from:
- ccsbBroadEn_05182
- DNA Barcode:
- AGACAATCCTCTACTTCCCTGTGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FAM76A (199870)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473578
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 199870 | FAM76A | family with sequence simila... | NM_152660.3 | 100% | 100% | |
| 2 | human | 199870 | FAM76A | family with sequence simila... | NM_001143914.2 | 90.5% | 90.5% | 508_509ins87 |
| 3 | human | 199870 | FAM76A | family with sequence simila... | NM_001143912.2 | 90% | 89.7% | 147_248del |
| 4 | human | 199870 | FAM76A | family with sequence simila... | NM_001143913.2 | 81.5% | 81.2% | 147_248del;610_611ins87 |
| 5 | human | 199870 | FAM76A | family with sequence simila... | NM_001143915.2 | 73.9% | 73.9% | 200_201ins153;355_356ins87 |
| 6 | human | 199870 | FAM76A | family with sequence simila... | XM_017000543.2 | 72.4% | 59.8% | (many diffs) |
| 7 | mouse | 230789 | Fam76a | family with sequence simila... | NM_145553.2 | 91.6% | 98.3% | (many diffs) |
| 8 | mouse | 230789 | Fam76a | family with sequence simila... | NM_001163792.1 | 82.4% | 88.9% | (many diffs) |
| 9 | mouse | 230789 | Fam76a | family with sequence simila... | XM_006538733.3 | 80.5% | 86.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 987
- ORF length:
- 921
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcgctctac gcctgcacca agtgccacca gcgcttcccc ttcgaggcgc 121 tgtctcaggg gcagcagctg tgcaaggaat gtcggattgc acaccctgtt gtgaagtgca 181 cctactgcag gactgagtac cagcaggaga gtaaaaccaa tacaatatgc aagaaatgtg 241 ctcagaacgt gcagttgtat ggaacgccca aaccttgtca gtattgcaac ataattgcag 301 catttattgg gaataaatgc cagcgctgca caaattcaga aaagaagtat ggaccaccct 361 attcttgtga acagtgcaag cagcagtgtg catttgacag gaaagatgat agaaagaagg 421 tagatgggaa attgctgtgc tggctgtgca cactttcata caaacgggtc cttcagaaga 481 ccaaagagca gaggaaacac ctgagtagct cttctcgtgc tggCCACCAG GAGAAGGAGC 541 AGTATAGTCG CCTGAGTGGT GGTGGCCATT ATAACAGCCA GAAAACACTT TCTACATCTT 601 CAATTCAAAA TGAAATCCCA AAGAAAAAGT CCAAGTTTGA GTCAATCACA ACTAATGGAG 661 ACAGCTTCTC CCCAGACCTG GCTCTGGACT CACCAGGCAC TGACCACTTT GTCATCATTG 721 CCCAACTGAA GGAAGAAGTG GCTACCCTGA AGAAGATGTT GCATCAAAAG GATCAAATGA 781 TTTTAGAGAA AGAGAAGAAG ATTACAGAGT TGAAGGCTGA TTTTCAGTAC CAGGAATCGC 841 AGATGAGAGC CAAAATGAAC CAGATGGAGA AAACCCACAA AGAAGTCACA GAACAACTGC 901 AGGCCAAAAA CCGAGAGCTC CTGAAGCAGG CAGCTGCTTT GTCCAAGAGC AAGAAGTCAG 961 AGAAGTCAGG AGCTATAACC TCTCCATGCC CAACTTTCTT GTACAAAGTG GTTGATATCG 1021 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1081 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAAGACAATC 1141 CTCTACTTCC CTGTGCACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1201 aagatt