Construct: ORF TRCN0000473581
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000516.1_s317c1
- Derived from:
- ccsbBroadEn_01372
- DNA Barcode:
- TTGATCGTTGCTACCCGTAAGCGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RALB (5899)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473581
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5899 | RALB | RAS like proto-oncogene B | NM_001369400.1 | 100% | 100% | |
| 2 | human | 5899 | RALB | RAS like proto-oncogene B | NM_002881.3 | 100% | 100% | |
| 3 | human | 5899 | RALB | RAS like proto-oncogene B | XM_005263728.1 | 100% | 100% | |
| 4 | human | 5899 | RALB | RAS like proto-oncogene B | XM_011511574.1 | 84.3% | 86.8% | (many diffs) |
| 5 | mouse | 64143 | Ralb | v-ral simian leukemia viral... | NM_022327.5 | 85.2% | 95.1% | (many diffs) |
| 6 | mouse | 64143 | Ralb | v-ral simian leukemia viral... | XM_006529782.1 | 85.2% | 95.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 684
- ORF length:
- 618
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tgccaacaag agtaagggcc agagctcctt ggccctccac aaggtgatca 121 tggttggcag cggaggcgtt ggcaagtcag ccctGACGCT TCAGTTCATG TATGACGAGT 181 TTGTAGAAGA CTATGAACCT ACCAAAGCTG ACAGTTATAG AAAGAAAGTG GTTCTTGATG 241 GGGAAGAAGT TCAGATAGAT ATTCTGGACA CCGCTGGGCA AGAGGACTAC GCAGCCATTC 301 GAGATAACTA CTTTCGGAGT GGGGAAGGGT TTCTTCTTGT GTTCTCAATC ACAGAACATG 361 AATCCTTTAC AGCAACTGCC GAATTCAGGG AACAGATTCT CCGTGTGAAG GCTGAAGAAG 421 ATAAAATTCC ACTGCTCGTC GTGGGAAACA AGTCTGACCT AGAGGAGCGG AGGCAGGTGC 481 CTGTGGAGGA GGCCAGGAGT AAAGCCGAAG AGTGGGGCGT GCAGTACGTG GAGACGTCAG 541 CGAAGACCCG GGCCAACGTG GACAAGGTGT TCTTTGACCT AATGAGAGAA ATCAGAACAA 601 AGAAGATGTC AGAAAACAAA GACAAGAATG GCAAGAAAAG CAGCAAGAAC AAGAAAAGTT 661 TTAAAGAAAG ATGTTGCTTA CTATGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 721 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 781 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT TGATCGTTGC 841 TACCCGTAAG CGAACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 901 att