Construct: ORF TRCN0000473597
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005574.1_s317c1
- Derived from:
- ccsbBroadEn_02051
- DNA Barcode:
- GCAGCGTCAATGTAGAGTAAAGAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- WASF1 (8936)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473597
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8936 | WASF1 | WASP family member 1 | NM_001024934.1 | 100% | 100% | |
| 2 | human | 8936 | WASF1 | WASP family member 1 | NM_001024935.1 | 100% | 100% | |
| 3 | human | 8936 | WASF1 | WASP family member 1 | NM_001024936.1 | 100% | 100% | |
| 4 | human | 8936 | WASF1 | WASP family member 1 | NM_003931.3 | 100% | 100% | |
| 5 | mouse | 83767 | Wasf1 | WAS protein family, member 1 | NM_031877.3 | 90.9% | 98.3% | (many diffs) |
| 6 | mouse | 83767 | Wasf1 | WAS protein family, member 1 | XM_006512932.2 | 90.9% | 98.3% | (many diffs) |
| 7 | mouse | 83767 | Wasf1 | WAS protein family, member 1 | XM_006512933.2 | 90.8% | 98.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1743
- ORF length:
- 1677
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc gctagtgaaa agaaacatcg atcctaggca cttgtgccac acagcactgc 121 ctagaggcat taagaatgaa ctggaatgtg taaccaatat ttccttggca aatataatta 181 gacaactaag tagcctaagt aaatatgctg aagatatatt tggagaatta ttcaatgaag 241 cacatagttt ttccttcaga gtcaactcat tgcaagaacg tgtggaccgt ttatctgtta 301 gtgttacaca gcttgatcca aaggaagaag aattgtcttt gcaagatata acaatgagga 361 aagctttccg aagttctaca attcaagacc agcagctttt cgatcgcaag actttgccta 421 ttccattaca ggagacgtac gatgtttgtg aacagcctcc acctctcaat atactcactc 481 cttatagaga tgatggtaaa gaaggtctga agttttatac caatccttcg tatttctttg 541 atctatggaa agaaaaaatg ttgcaagata cagaggataa gaggaaggaa aagaggaagc 601 agaagcagaa aaatctagat cgtcctcatg aaccagaaaa agtgccaaga gcacctcatg 661 acaggcggcg agaatggcag aagctggccc aaggtccaga gctggctgaa gatgatgcta 721 atctcttaca taagcatatt gaagttgcta atggcccagc ctctcatttt gaaacaagac 781 ctcagacata cgtggatcat atggatggat cttactcact ttctgccttg ccatttagtc 841 agatgagtga gcttctgact agagctgagg aaagggtatt agtcagacca catgaaccac 901 ctccacctcc accaatgcat ggagcaggag atgcaaaacc gatacccacc tgtatcagtt 961 ctgctacagg tttgatagaa aatcgccctc agtcaccagc tacaggcaga acacctgtgt 1021 ttgtgagccc cactccccca cctcctccac cacctcttcc atctgccttg tcaacttcct 1081 cattaagagc ttcaatgact tcaactcctc cccctccagt acctccccca cctccacctc 1141 cagccactgc tttgcaagct ccagcagtac caccacctcc agctcctctt cagattgccc 1201 ctggagttct tcacccagct cctcctccaa ttgcacctcc tctagtacag ccctctccac 1261 cagtagctag agctgcccca gtatgtgaga ctgtaccagt tcatccactc ccacaaggtg 1321 aagttcaggg gctgcctcca cccccaccac cgcctcctct gcctccacct ggcattcgac 1381 catcatcacc tgtcacagtt acagctcttg ctcaTCCTCC CTCTGGGCTA CATCCAACTC 1441 CATCTACTGC CCCAGGTCCC CATGTTCCAT TAATGCCTCC ATCTCCTCCA TCACAAGTTA 1501 TACCTGCTTC TGAGCCAAAG CGCCATCCAT CAACCCTACC TGTAATCAGT GATGCCAGGA 1561 GTGTGCTACT GGAAGCAATA CGAAAAGGTA TTCAGCTACG CAAAGTAGAA GAGCAGCGTG 1621 AACAGGAAGC TAAGCATGAA CGCATTGAAA ACGATGTTGC CACCATCCTG TCTCGCCGTA 1681 TTGCTGTTGA ATATAGTGAT TCGGAAGATG ATTCAGAATT TGATGAAGTA GATTGGTTGG 1741 AGTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1801 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1861 GGCTTTATAT ATCTTGTGGA AAGGACGAGC AGCGTCAATG TAGAGTAAAG AAACGCGTTA 1921 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt