Construct: ORF TRCN0000473609
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017532.1_s317c1
- Derived from:
- ccsbBroadEn_02783
- DNA Barcode:
- CGAGGATTCCATCGCAGCACAGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- POFUT1 (23509)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473609
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23509 | POFUT1 | protein O-fucosyltransferase 1 | NM_172236.2 | 100% | 100% | |
| 2 | human | 23509 | POFUT1 | protein O-fucosyltransferase 1 | NM_015352.2 | 48.2% | 47.1% | (many diffs) |
| 3 | human | 23509 | POFUT1 | protein O-fucosyltransferase 1 | XR_001754218.2 | 10.5% | (many diffs) | |
| 4 | mouse | 140484 | Pofut1 | protein O-fucosyltransferase 1 | NM_080463.3 | 43.7% | 42.4% | (many diffs) |
| 5 | mouse | 140484 | Pofut1 | protein O-fucosyltransferase 1 | XM_011239283.2 | 19.2% | 17.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 648
- ORF length:
- 582
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg cgccgccgcg tgggcacggc cgctgagcgt gtctttcctg ctgctgcttc 121 tgccgctccc ggggatgcct gcgggctcct gggacccggc cggttaccTG CTCTACTGCC 181 CCTGCATGGG GCGCTTTGGG AACCAGGCCG ATCACTTCTT GGGCTCTCTG GCATTTGCAA 241 AGCTGCTAAA CCGTACCTTG GCTGTCCCTC CTTGGATTGA GTACCAGCAT CACAAGCCTC 301 CTTTCACCAA CCTCCATGTG TCCTACCAGA AGTACTTCAA GCTGGAGCCC CTCCAGGCTT 361 ACCATCGGGT CATCAGCTTG GAGGATTTCA TGGAGAAGCT GGCACCCACC CACTGGCCCC 421 CTGAGAAGCG GGTGGCATAC TGCTTTGAGG TGGCAGCCCA GCGAAGCCCA GATAAGAAGA 481 CGTGCCCCAT GAAGGAAGGA AACCCCTTTG GCCCATTCTG GGATCAGTTT CATGTGAGTT 541 TCAACAAGTC GGAGCTTTTT ACAGGCATTT CCTTCAGTGC TTCCTACAGA GAACAATGGA 601 GCCAGAGGCG TGAGAATCAC TCCTGTGTTA CCTTACTCTT CCCAAGGTGC CCAACTTTCT 661 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 721 CGTAGTAATG AACTAGTCCG TAACTTGAAG TATTTCGATT TCTTGGCTTT ATATATCTTG 781 TGGAAAGGAC GACGAGGATT CCATCGCAGC ACAGCAACGC GTTAAGTCga caatcaacct 841 ctggattaca aaatttgtga aagatt