Construct: ORF TRCN0000473621
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009955.1_s317c1
- Derived from:
- ccsbBroadEn_06007
- DNA Barcode:
- CTACGGTGTAACCGCTGGTACTCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CISH (1154)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473621
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1154 | CISH | cytokine inducible SH2 cont... | NM_145071.3 | 99.7% | 100% | 402C>T;657T>C |
| 2 | human | 1154 | CISH | cytokine inducible SH2 cont... | XM_011533329.2 | 96.7% | 93.6% | (many diffs) |
| 3 | human | 1154 | CISH | cytokine inducible SH2 cont... | NM_013324.6 | 92.2% | 91.6% | (many diffs) |
| 4 | mouse | 12700 | Cish | cytokine inducible SH2-cont... | NM_009895.4 | 86% | 90.6% | (many diffs) |
| 5 | mouse | 12700 | Cish | cytokine inducible SH2-cont... | XM_006511635.1 | 67.7% | 69.4% | (many diffs) |
| 6 | mouse | 12700 | Cish | cytokine inducible SH2-cont... | NM_001317354.1 | 53.4% | 58.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 840
- ORF length:
- 774
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt cctctgcgtt cagggacctc gtcctttgct ggctgtggag cggactgggc 121 agcggcccct gtgggccccg tccctggaac tgcccaagcc agtcatgcag cccttgcctg 181 ctggggcctt cctcgaggag gtggcagagg gtaccccagc ccagacagag agtgagccaa 241 aggtgctgga cccagaggag gatctgctgt gcatagccaa gaccttctcc taccttcggg 301 aatctggctg gtattGGGGT TCCATTACGG CCAGCGAGGC CCGACAACAC CTGCAGAAGA 361 TGCCAGAAGG CACGTTCTTA GTACGTGACA GCACGCACCC CAGCTACCTG TTCACGCTGT 421 CAGTGAAAAC CACTCGTGGC CCCACCAATG TACGCATTGA GTATGCTGAC TCCAGCTTCC 481 GTCTGGACTC CAACTGCTTG TCCAGGCCAC GCATCCTGGC CTTTCCGGAT GTGGTCAGCC 541 TTGTGCAGCA CTATGTGGCC TCCTGCACTG CTGATACCCG AAGCGACAGC CCCGATCCTG 601 CTCCCACCCC GGCCCTGCCT ATGCCTAAGG AGGATGCGCC TAGTGACCCA GCACTGCCTG 661 CTCCTCCACC AGCCACTGCT GTACACCTAA AACTGGTGCA GCCCTTTGTA CGCAGAAGCA 721 GCGCCCGCAG CCTGCAACAC CTGTGCCGCC TTGTCATCAA CCGTCTGGTG GCCGACGTGG 781 ACTGCCTGCC ACTGCCCCGG CGCATGGCCG ACTACCTCCG ACAGTACCCC TTCCAGCTCT 841 GCCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 901 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 961 TTTATATATC TTGTGGAAAG GACGACTACG GTGTAACCGC TGGTACTCGA CGCGTTAAGT 1021 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt