Construct: ORF TRCN0000473624
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000247.1_s317c1
- Derived from:
- ccsbBroadEn_11136
- DNA Barcode:
- ATTCTTAGGCATGTTGATGCACAT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC6A11 (6538)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473624
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6538 | SLC6A11 | solute carrier family 6 mem... | NM_001317406.2 | 100% | 100% | |
| 2 | human | 6538 | SLC6A11 | solute carrier family 6 mem... | XM_011534033.2 | 54.5% | 54.5% | 625_1143del |
| 3 | human | 6538 | SLC6A11 | solute carrier family 6 mem... | NM_014229.3 | 32.9% | 32.9% | 625_1896del |
| 4 | mouse | 243616 | Slc6a11 | solute carrier family 6 (ne... | NM_172890.3 | 28.4% | 30.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 690
- ORF length:
- 624
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac ggcggagaag gcgctgcccc tgggcaatgg gaaggctgct gaggaggcgc 121 gggagtccga ggcgccgggt ggcggctgca gcagcggggg cgcggcgccc gcgcgccacc 181 cgcgcgtcaa gcgcgacaag gcggtccacg agcgcggcca ctggaacaac aaggtggagt 241 tcgtgctgag cgtggccggg gagatcattg ggctgggcaa cgtgtggcgc ttcccctacc 301 tgtgctacaa gaacggagga ggggcattcc tgattcccta cgtggtgttt tttatttgct 361 gtggaattcc tgtttttttc ctggagacag ctctggggca gttcacaagt gaaggtggca 421 ttacgtgttg gaggaaagtt tgccctttat ttgaaggcat tggctatgca acacaggtga 481 ttgaggccca tctgaatgtg tactacatca tcatcctggc atgggccatt ttttacctga 541 gcaactgctt cactacTGAG CTACCCTGGG CTACCTGTGG GCATGAGTGG AACACAGAGA 601 ATTGTGTGGA GTTCCAGAAA CTGAATGTGA GCAACTACAG CCATGTGTCT CTGCAGAATG 661 CCACCTCCCC TGTCATGGAG TTTTGGGAGC CCAACTTTCT TGTACAAAGT GGTTGATATC 721 GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG 781 TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAATTCTTA 841 GGCATGTTGA TGCACATACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg 901 aaagatt