Construct: ORF TRCN0000473633
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004566.1_s317c1
- Derived from:
- ccsbBroadEn_15153
- DNA Barcode:
- TCATAAGATTACTGACTTCACCCA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- LONRF3 (79836)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473633
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79836 | LONRF3 | LON peptidase N-terminal do... | NM_001031855.3 | 99.6% | 23.4% | (many diffs) |
| 2 | human | 79836 | LONRF3 | LON peptidase N-terminal do... | NM_024778.5 | 94.3% | 24.7% | (many diffs) |
| 3 | human | 79836 | LONRF3 | LON peptidase N-terminal do... | XM_005262476.2 | 93.3% | 21.9% | (many diffs) |
| 4 | human | 79836 | LONRF3 | LON peptidase N-terminal do... | XM_011531398.2 | 74.7% | 26.8% | (many diffs) |
| 5 | human | 79836 | LONRF3 | LON peptidase N-terminal do... | XM_017029845.1 | 74.5% | 31.2% | (many diffs) |
| 6 | human | 79836 | LONRF3 | LON peptidase N-terminal do... | XR_001755724.2 | 74.3% | (many diffs) | |
| 7 | human | 79836 | LONRF3 | LON peptidase N-terminal do... | NR_110311.1 | 69.3% | (many diffs) | |
| 8 | human | 79836 | LONRF3 | LON peptidase N-terminal do... | NM_001289109.1 | 55.5% | 7% | 0_1ins923;15_16delACinsNA;136_291del |
| 9 | human | 79836 | LONRF3 | LON peptidase N-terminal do... | XR_938554.2 | 26.8% | (many diffs) | |
| 10 | human | 79836 | LONRF3 | LON peptidase N-terminal do... | XR_001755725.1 | 20.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 672
- ORF length:
- 603
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggagtcagta cggatcgaac agatgctgag cttgcccgcc gaggtcagca 121 gcgacaactt ggagtcggcg gagcgagggg catcagcggc ccaagtagac atgggccccc 181 acccaaaggt ggctgcagag ggccccgcac ctctaccgac gcgggagcca gagcaagagc 241 agtctccggg gacctcaacg ccggagagca aagtcctgct cacgcaggca gacgccttgg 301 cgtcccgggg gcgaatccgt gaagccctcg aggtgtatag acagctctcc gagcggcagc 361 agctggtggc tgagcagctg gagcagctgg tgcgctgcct ggcggagaaa gtcccgcaag 421 gcgaggcgct ggcgccggcg cccccggacg agggtagcac tgcaagcggc accgtggcgg 481 cggaagagac ggggggcgcc gcggcggcgg cggccaccga ggtgtgggac ggctttaagt 541 gccggaaatg tcatgggttt ctatcagacc ccgttccttg tcgtgtggcc acaccttttg 601 taaactgtgc ctggaacgtg ggcgggccgc cgaccggcgc tgtgcgctgt gcggggtcaa 661 gctctccgcc ttgatggtgg ccactgggcg ggcgcgtgga gcccggcggg ctgggcagca 721 gccgccgccg ccgctgcgag tcaacgtggt gctcagcggc ctcctcggca agttgtttcc 781 aggcccagcg cgagcgtcgc aactccggca cgagggcaac cgactgtacc gcgagcgcca 841 ggtggaggcg gcactgctca agtacaacga ggcagttaag ttggctccaa atgaccactt 901 gctttatagc aaccggtctc agatttattt caccttggag tctcatgaga atgcactgca 961 tgatgcagaa atagcatgta agctccgccc gatgggtttt aaggcnaatt tcagaaaagc 1021 ccaagcctta gccaccctag gcaaggtgga ggaggcacta agggagtttc tctactgtgt 1081 atcccttgat ggaaagaaca agagagcaag atgtgaagcc caaagagaca atctggagct 1141 cccacattgt tctagtcagg aggaagcagc agccagggga gatggcagca gtctgatgga 1201 cccagctaaa gtgaaggggg atggtcagca gcaccacatg aaagaccagg aagaagagga 1261 ggagaagtgg gatgctacct ctccaaaagc tgcttccagc aagactggaa aatgccagga 1321 aaagaaaagg aaacattgcc agattgaatc ccaagaagaa acggggatgc ctaataaagc 1381 ctccaagcaa gatcctccca ctgatcaggg ggacaaacct gctctcagtt taccacttgc 1441 atctttcgac gcatctgacc ttgaatgcgc tctatgtatg agattattct atgagccagt 1501 cacaacacct tgcgggcata ctttttgctt aaaatgccta gaaagatgcc tagatcacaa 1561 cgcaaagtgt ccattgtgca aagacggtct ttcacagtgc ttggcatcaa gaaaatacag 1621 caaaaatgta ataatggagg agctcatagc taaattcctt ccagaagaac tgaaggaacg 1681 aaggaagctt tatgaagagg aaatggaaga actttctaac cttaataaga atgtgcctat 1741 tttcgtgtgt actatggcct atcccaccgt tccttgtccc ctgcacatct ttgagccttg 1801 ttaccgcctg atgattcGTA GATGCATTGA GACAGGCACG AGACAGTTTG GCATGTGCCT 1861 TGGAGATCCT GTCAAAGGGT TTGCAGAATA TGGCTGCATC CTAGAGATCA GAAATGTTCA 1921 ATTCTTTGCT GATGGCCGCT CAGTGGTTGA CAGCATAGGC AAGAGGCGCT TCAGGGTGCT 1981 CCATCAGAGC CAGCGGGATG GCTACAACAC AGCCGACATT GAATACATTG AAGACCAAAA 2041 GGTTCAGGGA GAGGATTGTG CTGAGCTCAT GGGATTACAT AACTGTGTCT ATCAGCAAGC 2101 ATCATTGTGG TTTCATTCGC TCAAATTATC CCTAAAGAAT CGGATACTCA ATCACTTTGG 2161 TCCCATGCCG GAGAAAGACG CCGATCCTCA GATGAACCCG AATGGCCCAG CCTGGTGCTG 2221 GTGGATGTTA GCAGTTCTTC CCTTGGAAAG CCGAGCTCAG CTCCCCTTCC TAGCAATGAG 2281 GTCCTTAAAG GACAGACTGA ATGGTATTCG ACGAGTCCTG GCCTTCATAT CCCGAAACCA 2341 AAACTTGCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT 2401 CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC 2461 TTGGCTTTAT ATATCTTGTG GAAAGGACGA TCATAAGATT ACTGACTTCA CCCAACGCGT 2521 TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt