Construct: ORF TRCN0000473635
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000443.1_s317c1
- Derived from:
- ccsbBroadEn_14273
- DNA Barcode:
- CTGCGACGCGGTACTGACGAATAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- JHY (79864)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473635
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79864 | JHY | junctional cadherin complex... | NM_199124.2 | 82.3% | 82% | 1_162del;910T>G |
| 2 | human | 79864 | JHY | junctional cadherin complex... | NM_001363088.1 | 33.4% | 31.5% | (many diffs) |
| 3 | human | 79864 | JHY | junctional cadherin complex... | NM_001363087.1 | 32.7% | 32% | (many diffs) |
| 4 | human | 79864 | JHY | junctional cadherin complex... | NM_001363089.1 | 31.6% | 30.9% | (many diffs) |
| 5 | human | 79864 | JHY | junctional cadherin complex... | NM_024806.4 | 31.6% | 30.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 825
- ORF length:
- 759
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtg ccattctgag tttgatgatc gaatccgggg caacggtatg gagcccgaca 121 gcttagacga ggaggaaagc cctcgatggg gaagcctgca cgagatggaa gaggaagcaa 181 gtggaaaagc agctcagatg gctcgcgagc aaaaccacca tacctgggac cagggcgcca 241 ataacaggca acaaccaata gaagacaaat attcagacct ccgcTATGAC CCGAACTGGA 301 AGAGTAAGAA GGAGGAAGGG CAGCTGCTGT CTGTGGAAGC GTTGCCGGAG TCCACGGACA 361 GCTCTTTAGA AAATCTGCCT TTGGCTCCCC TCTACCCTTC CCAGGAGACG TCAATGGAAC 421 TCTCCGGGGG AAAAGGCGAG CAGAAAGAGA GTCCACAGAG TGCAGCTTCT TTACTTGGTA 481 GTGAATTTTT AAGCCCAAAC TATGAGCATG GTGCCCGTCG CAGCAAGCCG TTTTCAGAGC 541 TGAGCGACAG TGACCTGGAG GAGAAGTCGA GCAGCCTTTC TCCGTACGTG AAGAGCTCAA 601 GTTCACATAA CGAGGTTTTC CTGCCGGGAT CACGTGGCCC TCGGCGAAGG AAATCCAAAC 661 AACATTTTGT GGAAAAAAAC AAGCTCACTT TGGGATTACC CACCCCGAAA ACGGACTCTT 721 ATCTTCAACT TCACAATAAA AAAAGAGGGG AATCTCATCC AGAACAGGTC ATTTTGAGAG 781 TAAATTCTCT CCCACGTGAT GGATTCAAAA CCGTTTGGAG CCAGTACCCA ACTTTCTTGT 841 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 901 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 961 GAAAGGACGA CTGCGACGCG GTACTGACGA ATATACGCGT TAAGTCgaca atcaacctct 1021 ggattacaaa atttgtgaaa gatt