Construct: ORF TRCN0000473643
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003389.1_s317c1
- Derived from:
- ccsbBroadEn_01657
- DNA Barcode:
- TGTCCCACAGGAGCATGGAAGCCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TDO2 (6999)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473643
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6999 | TDO2 | tryptophan 2,3-dioxygenase | NM_005651.4 | 100% | 100% | |
| 2 | mouse | 56720 | Tdo2 | tryptophan 2,3-dioxygenase | NM_019911.2 | 83.2% | 88.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1284
- ORF length:
- 1218
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag tgggtgccca tttttaggaa acaactttgg atatactttt aaaaaactcc 121 ccgtagaagg cagcgaagaa gacaaatcac aaactggtgt gaatagagcc agcaaaggag 181 gtcttatcta tgggaactac ctgcatttgg aaaaagtttt gaatgcacaa gaactgcaaa 241 gtgaaacaaa aggaaataaa atccatgatg aacatctttt tatcataact catcaagctt 301 atgaactctg gtttaagcaa atcctctggg agttggattc tgttcgagag atctttcaga 361 atggccatgt cagagatgaa aggaacatgc ttaaggttgt ttctcggatg caccgagtgt 421 cagtgatcct gaaactgctg gtgcagcagt tttccattct ggagacgatg acagccttgg 481 acttcaatga cttcagagag tacttatctc cagcatcagg cttccagagt ttgcaattcc 541 gactattaga aaacaagata ggtgttcttc agaacatgag agtcccttat aacagaagac 601 attatcgtga taacttcaaa ggagaagaaa atgaactgct acttaaatct gagcaggaaa 661 agacacttct ggaattagtg gaggcatggc tggaaagaac tccaggttta gagccacatg 721 gatttaactt cTGGGGAAAG CTTGAAAAAA ATATCACCAG AGGCCTGGAA GAGGAATTCA 781 TAAGGATTCA GGCTAAAGAA GAGTCTGAAG AAAAAGAGGA ACAGGTGGCT GAATTTCAGA 841 AGCAAAAAGA GGTGCTACTG TCCTTATTTG ATGAGAAACG TCATGAACAT CTCCTTAGTA 901 AAGGTGAAAG ACGGCTGTCA TACAGAGCAC TTCAGGGAGC ATTGATGATA TATTTTTACA 961 GGGAAGAGCC TAGGTTCCAG GTGCCTTTTC AGTTGCTGAC TTCTCTTATG GACATAGATT 1021 CACTGATGAC CAAATGGAGA TATAACCATG TGTGCATGGT GCACAGAATG CTGGGCAGCA 1081 AAGCTGGCAC CGGTGGTTCC TCAGGCTATC ACTACCTGCG ATCAACTGTG AGTGATAGGT 1141 ACAAGGTATT TGTAGATTTA TTTAATCTTT CAACATACCT GATTCCCCGA CACTGGATAC 1201 CGAAGATGAA CCCAACCATT CACAAATTTC TATATACAGC AGAATACTGT GATAGCTCCT 1261 ACTTCAGCAG TGATGAATCA GATTACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1321 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1381 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT GTCCCACAGG 1441 AGCATGGAAG CCCACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1501 att