Construct: ORF TRCN0000473644
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012545.1_s317c1
- Derived from:
- ccsbBroadEn_01062
- DNA Barcode:
- TTCCAACATACCTGCAGATCGAAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NCK1 (4690)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473644
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4690 | NCK1 | NCK adaptor protein 1 | NM_001291999.2 | 100% | 100% | |
| 2 | human | 4690 | NCK1 | NCK adaptor protein 1 | NM_006153.6 | 100% | 100% | |
| 3 | human | 4690 | NCK1 | NCK adaptor protein 1 | XM_024453538.1 | 100% | 100% | |
| 4 | human | 4690 | NCK1 | NCK adaptor protein 1 | NM_001190796.3 | 81.4% | 80.3% | (many diffs) |
| 5 | mouse | 17973 | Nck1 | non-catalytic region of tyr... | NM_010878.3 | 92.8% | 98.9% | (many diffs) |
| 6 | mouse | 17973 | Nck1 | non-catalytic region of tyr... | XM_006510840.2 | 92.8% | 98.9% | (many diffs) |
| 7 | mouse | 17973 | Nck1 | non-catalytic region of tyr... | NM_001324530.1 | 76% | 79.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1197
- ORF length:
- 1131
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc agaagaagtg gtggtagtag ccaaatttga ttatgtggcc caacaagaac 121 aagagttgga catcaagaag aatgagagat tatggcttct ggatgattct aagtcctggt 181 ggcgagttcg aaattccatg aataaaacag gttttgtgcc ttctaactat gtggaaagga 241 aaaacagtgc tcggaaagca tctattgtga aaaacctaaa ggatacctta ggcattggaa 301 aagtgaaaag aaaacctagt gtgccagatt ctgcatctcc tgctgatgat agttttgttg 361 acccagggga acgtctctat gacctcaaca tgcccgctta tgtgaaattt aactacatgg 421 ctgagagaga ggatgaatta tcattgataa aggggacaaa ggtgatcgtc atggagaaat 481 gcagtgatgg gtggtggcgt ggtagctaca atggacaagt tggatggttc ccttcaaact 541 atgtaactga agaaggtgac agtcctttgg gtgaccatgt gggttctctg tcagagaaat 601 tagcagcagt cgtcaataac ctaaatactg ggcaagtgtt gcatgtggta caggctcttt 661 acccattcag ctcatctaat gatgaagaac ttaatttcga gaaaggagat gtaatggatg 721 ttattgaaaa acctgaaaat gacccagagt ggtggaaatg caggaagatc aatggtatgg 781 ttggtctagt accaaaaaac tatgttaccg ttatgcagaa taatccatta acttcaggtt 841 tggaaccatc acctccacag tgtgattaca ttaggccttc actcactgga aagtttgctg 901 gcaatccttg gtattatggc aaagtcacca ggcatcaagc agaaatggca ttaaatgaaa 961 gaggacatga aggggatttc ctcattcgtg atagtgaatc ttcgccaaat gatttctcag 1021 tatcactaaa agcacaaggg aaaaacaagc attttaaagt ccaactaaaa gagactgtct 1081 actgcattgg gcagcgtaaa ttcagcacca tggaagaact tgtagaacat taCAAAAAGG 1141 CACCAATTTT TACAAGTGAA CAAGGAGAAA AATTATATCT TGTCAAGCAT TTATCATGCC 1201 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1261 TCGATTCTAC GTAGTAATGG ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1321 ATATATCTTG TGGAAAGGAC GATTCCAACA TACCTGCAGA TCGAAAACGC GTTAAGTCga 1381 caatcaacct ctggattaca aaatttgtga aagatt