Construct: ORF TRCN0000473656
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014953.1_s317c1
- Derived from:
- ccsbBroadEn_06160
- DNA Barcode:
- GCTCTCATAGGGCATGCCCCCTCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ENO1 (2023)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473656
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2023 | ENO1 | enolase 1 | NM_001428.5 | 99.9% | 99.7% | 1290C>A |
| 2 | human | 2023 | ENO1 | enolase 1 | NM_001353346.3 | 97.6% | 97.2% | (many diffs) |
| 3 | human | 2023 | ENO1 | enolase 1 | NM_001201483.4 | 78.4% | 78.3% | 0_1ins279;1011C>A |
| 4 | mouse | 433182 | Eno1b | enolase 1B, retrotransposed | NM_001025388.1 | 88.1% | 94.4% | (many diffs) |
| 5 | mouse | 13806 | Eno1 | enolase 1, alpha non-neuron | NM_023119.2 | 88% | 94.4% | (many diffs) |
| 6 | mouse | 13806 | Eno1 | enolase 1, alpha non-neuron | XM_006538525.2 | 88% | 94.4% | (many diffs) |
| 7 | mouse | 13806 | Eno1 | enolase 1, alpha non-neuron | XM_017319953.1 | 85.8% | 92.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1368
- ORF length:
- 1302
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc tattctcaag atccatgcca gggagatctt tgactctcgc gggaatccca 121 ctgttgaggt tgatctcttc acctcaaaag gtctcttcag agctgctgtg cccagtggtg 181 cttcaactgg tatctatgag gccctagagc tccgggacaa tgataagact cgctatatgg 241 ggaagggtgt ctcaaaggct gttgagcaca tcaataaaac tattgcgcct gccctggtta 301 gcaagaaact gaacgtcaca gaacaagaga agattgacaa actgatgatc gagatggatg 361 gaacagaaaa taaatctaag tttggtgcga acgccattct gggggtgtcc cttgccgtct 421 gcaaagctgg tgccgttgag aagggggtcc ccctgtaccg ccacatcgct gacttggctg 481 gcaactctga agtcatcctg ccagtcccgg cgttcaatgt catcaatggc ggttctcatg 541 ctggcaacaa gctggccatg caggagttca tgatcctccc agtcggtgca gcaaacttca 601 gggaagccat gcgcattgga gcagaggttt accacaacct gaagaatgtc atcaaggaga 661 aatatgggaa agatgccacc aatgtggggg atgaaggcgg gtttgctccc aacatcctgg 721 agaataaaga aggcctggag ctgctgaaga ctgctattgg gaaagctggc tacactgata 781 aggtggtcat cggcatggac gtagcggcct ccgagttctt caggTCTGGG AAGTATGACC 841 TGGACTTCAA GTCTCCCGAT GACCCCAGCA GGTACATCTC GCCTGACCAG CTGGCTGACC 901 TGTACAAGTC CTTCATCAAG GACTACCCAG TGGTGTCTAT CGAAGATCCC TTTGACCAGG 961 ATGACTGGGG AGCTTGGCAG AAGTTCACAG CCAGTGCAGG AATCCAGGTA GTGGGGGATG 1021 ATCTCACAGT GACCAACCCA AAGAGGATCG CCAAGGCCGT GAACGAGAAG TCCTGCAACT 1081 GCCTCCTGCT CAAAGTCAAC CAGATTGGCT CCGTGACCGA GTCTCTTCAG GCGTGCAAGC 1141 TGGCCCAGGC CAATGGTTGG GGCGTCATGG TGTCTCATCG TTCGGGGGAG ACTGAAGATA 1201 CCTTCATCGC TGACCTGGTT GTGGGGCTGT GCACTGGGCA GATCAAGACT GGTGCCCCTT 1261 GCCGATCTGA GCGCTTGGCC AAGTACAACC AGCTCCTCAG AATTGAAGAG GAGCTGGGCA 1321 GCAAGGCTAA GTTTGCCGGC AGGAACTTCA GAAAACCCTT GGCCAAGTAC CCAACTTTCT 1381 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1441 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1501 GTGGAAAGGA CGAGCTCTCA TAGGGCATGC CCCCTCGACG CGTTAAGTCg acaatcaacc 1561 tctggattac aaaatttgtg aaagatt