Construct: ORF TRCN0000473657
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008898.1_s317c1
- Derived from:
- ccsbBroadEn_00534
- DNA Barcode:
- TTACTCTATCCGTTGGAAATCTAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FABP1 (2168)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473657
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2168 | FABP1 | fatty acid binding protein 1 | NM_001443.3 | 100% | 100% | |
| 2 | mouse | 14080 | Fabp1 | fatty acid binding protein ... | NM_017399.4 | 85.9% | 84.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 120
- ORF end:
- 501
- ORF length:
- 381
- Sequence:
-
1 GAGTTTGGAT CTTGGTTCAT TCTCAAGCCT CAGACAGTGG TTCAAAGTTT TTTTTCTTCC 61 ATTTCAGGTG TCGTGAGGCT AGCATCGATT GATCAACAAG TTTGTACAAA AAAGTTGGCA 121 TGAGTTTCTC CGGCAAGTAC CAACTGCAGA GCCAGGAAAA CTTTGAAGCC TTCATGAAGG 181 CAATCGGTCT GCCGGAAGAG CTCATCCAGA AGGGGAAGGA TATCAAGGGG GTGTCGGAAA 241 TCGTGCAGAA TGGGAAGCAC TTCAAGTTCA CCATCACCGC TGGGTCCAAA GTGATCCAAA 301 ACGAATTCAC GGTGGGGGAG GAATGTGAGC TGGAGACAAT GACAGGGGAG AAAGTCAAGA 361 CAGTGGTTCA GTTGGAAGGT GACAATAAAC TGGTGACAAC TTTCAAAAAC ATCAAGTCTG 421 TGACCGAACT CAACGGCGAC ATAATCACCA ATACCATGAC ATTGGGTGAC ATTGTCTTCA 481 AGAGAATCAG CAAGAGAATT TACTCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 541 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 601 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATTAC TCTATCCGTT 661 GGAAATCTAG ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt