Construct: ORF TRCN0000473670
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014995.1_s317c1
- Derived from:
- ccsbBroadEn_01763
- DNA Barcode:
- CCAGACTCTCATTCTTATGATCGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- VCAM1 (7412)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473670
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7412 | VCAM1 | vascular cell adhesion mole... | NM_001078.4 | 99.9% | 100% | 2208G>A |
| 2 | human | 7412 | VCAM1 | vascular cell adhesion mole... | NM_001199834.1 | 91.5% | 91.6% | 152_153ins186;2022G>A |
| 3 | human | 7412 | VCAM1 | vascular cell adhesion mole... | NM_080682.2 | 87.5% | 87.4% | 928_929ins276;1932G>A |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2283
- ORF length:
- 2217
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc tgggaagatg gtcgtgatcc ttggagcctc aaatatactt tggataatgt 121 ttgcagcttc tcaagctttt aaaatcgaga ccaccccaga atctagatat cttgctcaga 181 ttggtgactc cgtctcattg acttgcagca ccacaggctg tgagtcccca tttttctctt 241 ggagaaccca gatagatagt ccactgaatg ggaaggtgac gaatgagggg accacatcta 301 cgctgacaat gaatcctgtt agttttggga acgaacactc ttacctgtgc acagcaactt 361 gtgaatctag gaaattggaa aaaggaatcc aggtggagat ctactctttt cctaaggatc 421 cagagattca tttgagtggc cctctggagg ctgggaagcc gatcacagtc aagtgttcag 481 ttgctgatgt atacccattt gacaggctgg agatagactt actgaaagga gatcatctca 541 tgaagagtca ggaatttctg gaggatgcag acaggaagtc cctggaaacc aagagtttgg 601 aagtaacctt tactcctgtc attgaggata ttggaaaagt tcttgtttgc cgagctaaat 661 tacacattga tgaaatggat tctgtgccca cagtaaggca ggctgtaaaa gaattgcaag 721 tctacatatc acccaagaat acagttattt ctgtgaatcc atccacaaag ctgcaagaag 781 gtggctctgt gaccatgacc tgttccagcg agggtctacc agctccagag attttctgga 841 gtaagaaatt agataatggg aatctacagc acctttctgg aaatgcaact ctcaccttaa 901 ttgctatgag gatggaagat tctggaattt atgtgtgtga aggagttaat ttgattggga 961 aaaacagaaa agaggtggaa ttaattgttc aagagaaacc atttactgtt gagatctccc 1021 ctggaccccg gattgctgct cagattggag actcagtcat gttgacatgt agtgtcatgg 1081 gctgtgaatc cccatctttc tcctggagaa cccagataga cagccctctg agcgggaagg 1141 tgaggagtga ggggaccaat tccacgctga ccctgagccc tgtgagtttt gagaacgaac 1201 actcttatct gtgcacagtg acttgtggac ataagaaact ggaaaaggga atccaggtgg 1261 agctctactc attccctaga gatccagaaa tcgagatgag tggtggcctc gtgaatggga 1321 gctctgtcac tgtaagctgc aaggttccta gcgtgtaccc ccttgaccgg ctggagattg 1381 aattacttaa gggggagact attctggaga atatagagtt tttggaggat acggatatga 1441 aatctctaga gaacaaaagt ttggaaatga ccttcatccc taccattgaa gatactggaa 1501 aagctcttgt ttgtcaggct aagttacata ttgatgacat ggaattcgaa cccaaacaaa 1561 ggcagagtac gcaaacactt tatgtcaatg ttgcccccag agatacaacc gtcttggtca 1621 gcccttcctc catcctggag gaaggcagtt ctgtgaatat gacatgcttg agccagggct 1681 ttcctgctcc gaaaatcctg tggagcaggc agctccctaa cggggagcta cagcctcttt 1741 ctgagaatgc aactctcacc ttaatttcta caaaaatgga agattctggg gtttatttat 1801 gtgaaggaat taaccaggct ggaagaagca gaaaggaagt ggaattaatt atccaagtta 1861 ctccaaaaga cataaaactt acagcttttc cttctgagag tgtcaaagaa ggagacactg 1921 tcatcatctc ttGTACATGT GGAAATGTTC CAGAAACATG GATAATCCTG AAGAAAAAAG 1981 CGGAGACAGG AGACACAGTA CTAAAATCTA TAGATGGCGC CTATACCATC CGAAAGGCCC 2041 AGTTGAAGGA TGCGGGAGTA TATGAATGTG AATCTAAAAA CAAAGTTGGC TCACAATTAA 2101 GAAGTTTAAC ACTTGATGTT CAAGGAAGAG AAAACAACAA AGACTATTTT TCTCCTGAGC 2161 TTCTCGTGCT CTATTTTGCA TCCTCCTTAA TAATACCTGC CATTGGAATG ATAATTTACT 2221 TTGCAAGAAA AGCCAACATG AAGGGGTCAT ATAGTCTTGT AGAAGCACAG AAATCAAAAG 2281 TGTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 2341 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 2401 GGCTTTATAT ATCTTGTGGA AAGGACGACC AGACTCTCAT TCTTATGATC GAACGCGTTA 2461 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt