Construct: ORF TRCN0000473678
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012917.1_s317c1
- Derived from:
- ccsbBroadEn_01301
- DNA Barcode:
- TGTAACCGCAAACGGCCGGTCAAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PRRG1 (5638)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473678
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5638 | PRRG1 | proline rich and Gla domain 1 | NM_000950.3 | 100% | 100% | |
| 2 | human | 5638 | PRRG1 | proline rich and Gla domain 1 | NM_001142395.2 | 100% | 100% | |
| 3 | human | 5638 | PRRG1 | proline rich and Gla domain 1 | NM_001173489.1 | 100% | 100% | |
| 4 | human | 5638 | PRRG1 | proline rich and Gla domain 1 | NM_001173490.1 | 100% | 100% | |
| 5 | human | 5638 | PRRG1 | proline rich and Gla domain 1 | NM_001173486.2 | 33.2% | 23.9% | (many diffs) |
| 6 | mouse | 546336 | Prrg1 | proline rich Gla (G-carboxy... | NM_001164275.2 | 90.5% | 92.6% | (many diffs) |
| 7 | mouse | 546336 | Prrg1 | proline rich Gla (G-carboxy... | NM_001289683.1 | 90.5% | 92.6% | (many diffs) |
| 8 | mouse | 546336 | Prrg1 | proline rich Gla (G-carboxy... | NM_027322.2 | 90.5% | 92.6% | (many diffs) |
| 9 | mouse | 546336 | Prrg1 | proline rich Gla (G-carboxy... | XM_011247631.2 | 90.5% | 92.6% | (many diffs) |
| 10 | mouse | 546336 | Prrg1 | proline rich Gla (G-carboxy... | XM_011247637.1 | 90.5% | 92.6% | (many diffs) |
| 11 | mouse | 546336 | Prrg1 | proline rich Gla (G-carboxy... | XM_011247638.1 | 90.5% | 92.6% | (many diffs) |
| 12 | mouse | 546336 | Prrg1 | proline rich Gla (G-carboxy... | XM_017318551.1 | 90.5% | 92.6% | (many diffs) |
| 13 | mouse | 546336 | Prrg1 | proline rich Gla (G-carboxy... | XM_006528142.3 | 81.8% | 82.9% | (many diffs) |
| 14 | mouse | 546336 | Prrg1 | proline rich Gla (G-carboxy... | XM_006528143.2 | 81.8% | 82.9% | (many diffs) |
| 15 | mouse | 546336 | Prrg1 | proline rich Gla (G-carboxy... | XM_006528144.2 | 81.8% | 82.9% | (many diffs) |
| 16 | mouse | 546336 | Prrg1 | proline rich Gla (G-carboxy... | XM_011247632.1 | 81.8% | 82.9% | (many diffs) |
| 17 | mouse | 546336 | Prrg1 | proline rich Gla (G-carboxy... | XM_011247633.2 | 81.8% | 82.9% | (many diffs) |
| 18 | mouse | 546336 | Prrg1 | proline rich Gla (G-carboxy... | XM_011247634.1 | 81.8% | 82.9% | (many diffs) |
| 19 | mouse | 546336 | Prrg1 | proline rich Gla (G-carboxy... | XM_011247635.1 | 81.8% | 82.9% | (many diffs) |
| 20 | mouse | 546336 | Prrg1 | proline rich Gla (G-carboxy... | XM_006528145.3 | 77.4% | 79.2% | (many diffs) |
| 21 | mouse | 546336 | Prrg1 | proline rich Gla (G-carboxy... | XM_017318550.1 | 70.9% | 71.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 720
- ORF length:
- 654
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg gagggttttc ctcacgggag aaaaagccaa ttccatatta aaacgctacc 121 caagagctaa tgggtttttt gaagaaataa gacagggcaa cattgagcgt gagtgcaaag 181 aagaattctg tacatttgaa gaagcaagag aagcttttga aaataatgaa aaaactaagg 241 agttttggag cacctacaca aaagcgcaac aaggggagag taaccgagga agtgactggt 301 ttcagtttta ccttaccttt ccgttaatct ttggcctctt cattatcctc cttgtcattt 361 tcctaatctg gagatgcttc ctaagaaaca aaactcgtag acagacagtg actgaaggcc 421 acattccttt ccctcagcac cttaatatta tcaccccacc ccccccacca gatgaagtgt 481 ttgacagcag tggattgtct ccaggcttTC TGGGATATGT AGTTGGGCGC TCAGATTCCG 541 TCTCTACTCG CCTGTCCAAT TGTGATCCCC CGCCAACCTA TGAGGAAGCC ACTGGCCAAG 601 TGAACCTGCA GAGGAGTGAA ACAGAACCTC ATTTAGACCC ACCCCCAGAG TATGAGGACA 661 TAGTCAACTC CAACTCAGCC AGTGCCATTC CTATGGTGCC TGTGGTCACC ACCATCAAAT 721 GCCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 781 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 841 TTTATATATC TTGTGGAAAG GACGATGTAA CCGCAAACGG CCGGTCAAAA CGCGTTAAGT 901 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt